Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Cdc2 p34 Antibody (17) - Western Blotting - Image 126885
Cdc2 p34 抗体 (17): sc-54. 未转染 293: sc-110760 (A), 转染人 Cdc2 p34 的 293: sc-128282 (B) 和 HeLa (C) 全细胞裂解液中 Cdc2 p34 表达的 Western 印迹分析.
Cdc2 p34 抗体 (17): sc-54. 未转染 293: sc-110760 (A), 转染人 Cdc2 p34 的 293: sc-128282 (B) 和 HeLa (C) 全细胞裂解液中 Cdc2 p34 表达的 Western 印迹分析.

Cdc2 p34 抗体 (17): sc-54

4.7(46)
寫評論提問

说明书
  • Cdc2 p34 抗体 17 是小鼠单克隆 IgG2a κ，Cdc2 p34抗体, 在746篇文献中引用，规格为200 µg/ml
  • 针对氨基酸224-230，在Cdc2 p34的中央区域进行定位，human起源
  • Cdc2 p34 抗体 (17) 推荐用于WB, IP, IF 和 IHC(P) ，检测 mouse, rat, human 和 Xenopus来源的Cdc2 p34
  • 抗Cdc2 p34抗体（17）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdc2 p34 (17): sc-54的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Cdc2 p34 抗体 (17) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc2 p34 抗体 (17) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Cdc 抗体 包括 Cdc11, Cdc123, Cdc13, Cdc14 Phosphatase, Cdc16, Cdc2, Cdc20B, Cdc25, Cdc26和Cdc27

Cdc2 p34 抗体 (17) 是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光和免疫组织化学检测小鼠、大鼠、人类和非洲爪蟾来源的 Cdc2 p34 蛋白。Cdc2 p34 (17) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdc2 p34，也称为细胞周期蛋白依赖性激酶1（CDK1），在调节细胞周期中起着至关重要的作用，特别是在从G2期到有丝分裂的过渡中。这种调节对于细胞正常分裂至关重要，可确保细胞在细胞分裂前准确复制DNA，从而防止基因组不稳定和潜在的肿瘤形成。在脊椎动物中，Cdc2 p34与各种细胞周期蛋白相互作用，这些蛋白对于激活和功能至关重要，突出了细胞周期控制的重要性。此外，其他细胞周期蛋白依赖性激酶（如Cdk2）的存在也突出了脊椎动物细胞周期调节的复杂性。Cdc2 p34 单克隆抗体（17）的检测能力使其成为研究细胞周期动态和相关过程的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc2 p34 抗体 (17) 参考文献:

  1. 人类细胞周期的两个阶段都需要 Cyclin A。  |  Pagano, M., et al. 1992. EMBO J. 11: 961-71. PMID: 1312467
  2. CDK2 编码一种 33 kDa 的细胞周期蛋白 A 相关蛋白激酶，在细胞周期中比 CDC2 表达得更早。  |  Elledge, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 2907-11. PMID: 1372993
  3. 新型单克隆抗体鉴定出人类 CDC2 的不同形式。  |  Lukás, J., et al. 1992. Eur J Biochem. 207: 169-76. PMID: 1628647
  4. 人类 D 型细胞周期蛋白  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
  5. 爪蟾卵母细胞成熟过程中的环蛋白及其伙伴  |  Kobayashi, H., et al. 1991. Cold Spring Harb Symp Quant Biol. 56: 437-47. PMID: 1840257
  6. cdc2 的可逆酪氨酸磷酸化：进入有丝分裂过程中伴随着去磷酸化激活。  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
  7. cdc2 激酶是一种核蛋白，对哺乳动物细胞的有丝分裂至关重要。  |  Riabowol, K., et al. 1989. Cell. 57: 393-401. PMID: 2541912
  8. 人细胞周期蛋白 cDNA 的分离：细胞周期蛋白 mRNA 和蛋白质在细胞周期中的调控以及与 p34cdc2 相互作用的证据。  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
  9. Cip1 通过抑制依赖细胞周期蛋白的激酶，阻止了爪蟾提取物中 DNA 复制的启动。  |  Strausfeld, UP., et al. 1994. Curr Biol. 4: 876-83. PMID: 7850420
  10. 氮芥诱导的人体细胞G2延迟对细胞周期蛋白A/cdk2和细胞周期蛋白B1/cdc2-激酶复合物的影响不同。  |  O'Connor, PM., et al. 1993. J Biol Chem. 268: 8298-308. PMID: 8463339
  11. CDK2和cdc2激酶及其共同伙伴细胞周期蛋白A在甲状腺上皮细胞中的表达和亚细胞定位：依赖环磷酸腺苷和不依赖环磷酸腺苷的细胞周期的比较。  |  Baptist, M., et al. 1996. J Cell Physiol. 166: 256-73. PMID: 8591985
  12. 在爪蟾卵提取物中，细胞周期蛋白 A 和细胞周期蛋白 E 都具有促进 S 期（SPF）的活性。  |  Strausfeld, UP., et al. 1996. J Cell Sci. 109 (Pt 6): 1555-63. PMID: 8799842

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc2 p34 抗体 (17)

sc-54
200 µg/ml
RMB2377.00

Cdc2 p34 (17): m-IgG Fc BP-HRP 套装

sc-528092
200 µg Ab; 10 µg BP
RMB2662.00

Cdc2 p34 (17): m-IgGκ BP-HRP 套装

sc-520390
200 µg Ab, 40 µg BP
RMB2662.00

Cdc2 p34 抗体 (17) AC

sc-54 AC
500 µg/ml, 25% agarose
RMB3129.00

Cdc2 p34 抗体 (17) HRP

sc-54 HRP
200 µg/ml
RMB2377.00

Cdc2 p34 抗体 (17) FITC

sc-54 FITC
200 µg/ml
RMB2482.00

Cdc2 p34 抗体 (17) PE

sc-54 PE
200 µg/ml
RMB2580.00

Cdc2 p34 抗体 (17) Alexa Fluor® 488

sc-54 AF488
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) Alexa Fluor® 546

sc-54 AF546
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) Alexa Fluor® 594

sc-54 AF594
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) Alexa Fluor® 647

sc-54 AF647
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) Alexa Fluor® 680

sc-54 AF680
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) Alexa Fluor® 790

sc-54 AF790
200 µg/ml
RMB2685.00

Cdc2 p34 抗体 (17) TRITC

sc-54 TRITC
200 µg/ml
RMB2407.00

Does this antibody cross react with CDK2?

Asked by: Gianmarco
Thank you for your question. We have no data showing cross-reactivity with CDK2.
Answered by: Tech Support Europe
Date published: 2024-10-08

Any information if this antibody will react bovine protein? Thank you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in Madin-Darby bovine kidney (MDBK) cells. Please refer to this reference on NCBI's PubMed, PMID: 9733854.
Answered by: Technical Support
Date published: 2017-08-02

Can this antibody be used to detect Cdc2 in yeast?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions about reactivity with unlisted species.
Answered by: Technical Service
Date published: 2016-12-22

Which version of this antibody should be used for IP, sc-54 or sc-54 AC?

Asked by: AbPolly
Thank you for your inquiry. If the only application you intend to use this antibody for is IP, then the agarose conjugated version sc-54 AC is a great choice. If you need to use the antibody for multiple applications such as IP and Western Blot, then the unconjugated version sc-54 is best.
Answered by: Technical Service
Date published: 2016-12-22
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_54, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from とてもきれいですMCF7細胞や他の細胞でウェスタンブロットによりCdk1の発現量を検討すると、特異的なシグナルが検出できました。
Date published: 2023-04-06
Rated 5 out of 5 by from Good for in vivo and in vitroTests: In mouse brain tissues and mouse primary brain cells PFA fixation only (10min cold) at 1:100 dilution Blocking buffer:0.1% Triton in PBS+10%FBS
Date published: 2022-09-09
Rated 5 out of 5 by from good antibodygood antibody clean and strong signal in western blot or IF
Date published: 2021-11-15
Rated 4 out of 5 by from Good enoughThe antibody works fine in IHC on FFPE. The best dilution is 1:400 using as antigen retrival EDTA buffer pH 8.0 in water bath at 98°C. The sample in the photos are bioptical sample from bladder carcinoma. The staining is heterogenous between cancer cells, mainly in the cytoplasm but occasionally found in nuclei. Magnification 20x and 40x.
Date published: 2021-04-27
Rated 4 out of 5 by from molto buonoQuesta anticorpo è stato acquistato il 9 Febbraio; risultato molto buono.
Date published: 2021-03-18
Rated 5 out of 5 by from Great immunoblotting antibodies!Although this antibody was raised against human Cdc2, it immunoblots Cdc2 in Xenopus egg extracts very well with low background staining. Although there is one cross-reactive band above 60 kDa, it is far enough from the Cdc2 signal to obtain clear cut results. /Users/jxkuang/Desktop/sc-54.png
Date published: 2021-01-27
Rated 4 out of 5 by from 对应条带清晰，特异性较高本抗体，我们试验中用于大鼠组织标本蛋白检测，条带较为清晰，对应分子量匹配，特异性较高，后期如果需要会继续购入。
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_46
  • loc_zh_CN, sid_54, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
Cdc2 p34 抗体 (17) is rated 4.7 out of 5 by 46.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_46
  • loc_zh_CN, sid_54, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 81ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。