Date published: 2025-10-23
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CD94 Antibody (H-3) - Western Blotting - Image 380827
CD94 抗体 (H-3): sc-390776. U-87 MG (A), HT-1080 (B), HL-60 (C) 和 BYDP (D) 全细胞裂解液中 CD94 表达的 Western 印迹分析.
CD94 Antibody (H-3) - Western Blotting - Image 380827
CD94 抗体 (H-3): sc-390776. U-87 MG (A), HT-1080 (B), HL-60 (C) 和 BYDP (D) 全细胞裂解液中 CD94 表达的 Western 印迹分析.
CD94 Antibody (H-3) - Western Blotting - Image 290889
CD94 抗体 (H-3): sc-390776. NK-92 (A) 和小鼠 PBL (B) 全细胞裂解液中 CD94 表达的 Western 印迹分析.
CD94 抗体 (H-3): sc-390776. U-87 MG (A), HT-1080 (B), HL-60 (C) 和 BYDP (D) 全细胞裂解液中 CD94 表达的 Western 印迹分析.

CD94 抗体 (H-3): sc-390776

4.3(7)
寫評論提問

说明书
  • CD94 抗体 H-3 是小鼠单克隆 IgG2a κ，CD94抗体, 在1篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列111-179位于human物种的CD94的C-terminus
  • CD94 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CD94
  • 抗CD94抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是CD94 Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD94 Antibody (H-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD94抗体（H-3）是一种IgG2a κ小鼠单克隆CD94抗体（也称为CD94抗体），它通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的CD94蛋白。CD94抗体（H-3）既可以以非偶联的抗CD94抗体形式存在，也可以以多种偶联形式的抗CD94抗体存在，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。自然杀伤细胞（NK）的活性受到多个受体家族成员的调节，这些受体能够识别I类MHC分子，如杀伤细胞抑制受体/白细胞免疫球蛋白样受体（KIR/LIR）家族和C型凝集素超家族。KIR/LIR家族包括p91A（也称为pp130或PIR-B，代表配对免疫球蛋白样受体-B）和p91B（也称为PIR-A）。p91A通过与SHP-1相互作用而作为抑制受体，而p91B则作为激活受体。CD94、NKG2和Ly-49是II型膜糖蛋白C型凝集素超家族的成员。CD94在NK细胞表面与NKG2异构体形成异二聚体，而Ly-49异构体则形成同二聚体。NKG2-D在NK细胞、γδT细胞和CD8+ αβ T细胞上表达，是应激诱导蛋白MICA的受体，MICA是一种经常在上皮肿瘤中表达的抗原。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD94 抗体 (H-3) 参考文献:

  1. 应激诱导型 MICA 受体 NKG2D 激活 NK 细胞和 T 细胞。  |  Bauer, S., et al. 1999. Science. 285: 727-9. PMID: 10426993
  2. 小鼠 Ly49 类凝集素受体对自然杀伤细胞的抑制作用  |  Salcedo, M. 1999. Curr Top Microbiol Immunol. 244: 97-105. PMID: 10453652
  3. 一种与杀伤细胞抑制受体同源的新型鼠细胞表面糖蛋白的分子克隆。  |  Hayami, K., et al. 1997. J Biol Chem. 272: 7320-7. PMID: 9054430
  4. NK 细胞上凝集素样受体的不同功能  |  Ryan, JC. and Seaman, WE. 1997. Immunol Rev. 155: 79-89. PMID: 9059884
  5. 自然杀伤细胞受体。  |  Long, EO. and Wagtmann, N. 1997. Curr Opin Immunol. 9: 344-50. PMID: 9203417
  6. HLA I 类特异性抑制受体。  |  Moretta, A. and Moretta, L. 1997. Curr Opin Immunol. 9: 694-701. PMID: 9368779
  7. 克隆小鼠 CD94 的同源物扩展了小鼠自然杀伤细胞上的 C 型凝集素家族。  |  Vance, RE., et al. 1997. Eur J Immunol. 27: 3236-41. PMID: 9464811
  8. 巨噬细胞中主要的 SHP-1 结合蛋白, 酪氨酸磷酸化蛋白是 KIR/LIR 家族的成员，也是 SHP-1 的底物。  |  Berg, KL., et al. 1998. Oncogene. 17: 2535-41. PMID: 9824165

订购信息

产品名称产品编号规格价格数量收藏夹

CD94 抗体 (H-3)

sc-390776
200 µg/ml
RMB2377.00

CD94 (H-3): m-IgG Fc BP-HRP 套装

sc-530259
200 µg Ab; 10 µg BP
RMB2662.00

CD94 (H-3): m-IgGκ BP-HRP 套装

sc-523679
200 µg Ab, 40 µg BP
RMB2662.00

CD94 (H-3): m-IgG2a BP-HRP 套装

sc-547467
200 µg Ab; 10 µg BP
RMB2662.00

CD94 抗体 (H-3) AC

sc-390776 AC
500 µg/ml, 25% agarose
RMB3129.00

CD94 抗体 (H-3) HRP

sc-390776 HRP
200 µg/ml
RMB2377.00

CD94 抗体 (H-3) FITC

sc-390776 FITC
200 µg/ml
RMB2482.00

CD94 抗体 (H-3) PE

sc-390776 PE
200 µg/ml
RMB2580.00

CD94 抗体 (H-3) Alexa Fluor® 488

sc-390776 AF488
200 µg/ml
RMB2685.00

CD94 抗体 (H-3) Alexa Fluor® 546

sc-390776 AF546
200 µg/ml
RMB2685.00

CD94 抗体 (H-3) Alexa Fluor® 594

sc-390776 AF594
200 µg/ml
RMB2685.00

CD94 抗体 (H-3) Alexa Fluor® 647

sc-390776 AF647
200 µg/ml
RMB2685.00

CD94 抗体 (H-3) Alexa Fluor® 680

sc-390776 AF680
200 µg/ml
RMB2685.00

CD94 抗体 (H-3) Alexa Fluor® 790

sc-390776 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of canine origin. Do you know if CD94 (H-3): sc-390776 mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 1 out of 5 by from Does not work for flow cytometryThe CD94 antibody did not work for flow cytometry with NK92MI cells
Date published: 2018-11-01
Rated 5 out of 5 by from IF detectionIF detection human Hela cell and we used this antibody to detect the expression of the target protein, CD94 is membrance marker, it shows membrance staining with dilution 1:400.
Date published: 2017-09-10
Rated 5 out of 5 by from specific bandUse the 1:1000 dilution in TBS + 0,02 % Tween 20 + 1 % skim milk. Band visible at 30 kDa seen.
Date published: 2017-08-19
Rated 4 out of 5 by from WBI have detect some non-specific bands at first .However they don’t interfere with the signal.I recommend incubating 2 h at Room temperature instead of 4℃over night. it can be avoided the non-specific bands.
Date published: 2017-07-29
Rated 5 out of 5 by from Nice bandI ever used anti-CD94 antibody at 1:800 dilution on murine heart tissue. Gives a nice band at 30 kDa as expected,I suggest not loading too much sample, maybe 50ug is better.
Date published: 2017-07-29
Rated 5 out of 5 by from IFI got this antibody from SC, and the staining works well.
Date published: 2016-10-25
Rated 5 out of 5 by from Produced positive Western blot data of CD94Produced positive Western blot data of CD94 expression in NK-92 and mouse PBL whole cell lysates. -SCBT QC
Date published: 2014-11-14
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CD94 抗体 (H-3) is rated 4.3 out of 5 by 7.
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