Date published: 2025-9-6
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CD8 Antibody (2.43) - Flow Cytometry - Image 5756
CD8 抗体 (2. 43) FITC: sc-18860 FITC. 小鼠外周血白细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2b: sc-2835 设置.
CD8 Antibody (2.43) - Flow Cytometry - Image 5756
CD8 抗体 (2. 43) FITC: sc-18860 FITC. 小鼠外周血白细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2b: sc-2835 设置.
CD8 Antibody (2.43) - Immunofluorescence - Image 4723
CD8 抗体 (2. 43): sc-18860. 甲醇固定的 CTLL-2 细胞免疫荧光染色, 显示膜染色.
CD8 Antibody (2.43) - Flow Cytometry - Image 5755
CD8 抗体 (2. 43) PE: sc-18860 PE. CTLL-2 细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2b: sc-2873 设置.
CD8 抗体 (2. 43) FITC: sc-18860 FITC. 小鼠外周血白细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2b: sc-2835 设置.

CD8 抗体 (2.43): sc-18860

5.0(6)
寫評論提問

说明书
  • CD8 抗体 2.43 是大鼠单克隆 IgG2b, 在14篇文献中引用，提供 200 µg/ml
  • 针对mouse来源的CTL克隆L3细胞
  • 推荐用于 mouse 来源的CD8 IF 和 FCM检测
  • 可提供偶联藻红蛋白, FITC, Alexa Fluor® 488 或者Alexa Fluor® 647 的产品, 应用于IF, IHC(P)和FCM
  • 看 CD8 (32-M4): sc-1177，可查看有标记的 CD8 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成CD8 抗体 (2.43)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD8 抗体包括 CD8-α 和 CD8-β

CD8抗体（2.43）是一种鼠单克隆IgG2b抗体，可通过免疫荧光（IF）和流式细胞术（FCM）检测小鼠样本中的CD8。CD8是一种重要的细胞表面糖蛋白，可形成异二聚体，通常以αα或αβ链复合物的形式存在，通过与抗原呈递细胞呈现的I类主要组织相容性复合物（MHC）分子结合，在免疫反应中发挥重要作用。这种相互作用对于表达CD8的T细胞毒性细胞至关重要，因为CD8可增强T细胞受体（TCR）对抗原的识别，从而增强对感染或恶性细胞的免疫反应。此外，表达CD8的T细胞与维持低水平HIV表达有关，这表明它们不仅对直接细胞毒性，而且对调节病毒载量都很重要。CD8能够促进抗原识别并增强TCR亲和力，这凸显了CD8在适应性免疫中的重要性，使抗CD8抗体（2.43）成为研究人员在小鼠模型中研究T细胞动态和免疫反应的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD8 抗体 (2.43) 参考文献:

  1. CD8+ TIL中线粒体动态的紊乱加剧了T细胞的衰竭。  |  Yu, YR., et al. 2020. Nat Immunol. 21: 1540-1551. PMID: 33020660
  2. 黑色素瘤中抗肿瘤CD8+ T细胞的表型、特异性和亲和力。  |  Oliveira, G., et al. 2021. Nature. 596: 119-125. PMID: 34290406
  3. CD8α-PILRA相互作用维持CD8+ T细胞的静止状态。  |  Zheng, L., et al. 2022. Science. 376: 996-1001. PMID: 35617401
  4. 碳源的可用性驱动CD8+ T细胞对营养的利用。  |  Kaymak, I., et al. 2022. Cell Metab. 34: 1298-1311.e6. PMID: 35981545
  5. 肿瘤免疫原性决定了CD8+ T细胞对免疫治疗的反应依赖于TCF1。  |  Escobar, G., et al. 2023. Cancer Cell. 41: 1662-1679.e7. PMID: 37625402
  6. HIV疫苗诱导抗原受体敏感性低的CD8+ T细胞。  |  Migueles, SA., et al. 2023. Science. 382: 1270-1276. PMID: 38096385
  7. 癌症中CD8+ T细胞分化的免疫代谢。  |  Shi, H., et al. 2024. Trends Cancer. 10: 610-626. PMID: 38693002
  8. DEPDC5通过限制mTORC1介导的嘌呤分解代谢来保护CD8+ T细胞免受铁死亡。  |  Li, S., et al. 2024. Cell Discov. 10: 53. PMID: 38763950
  9. IL-4驱动CD8+ CART细胞的衰竭。  |  Stewart, CM., et al. 2024. Nat Commun. 15: 7921. PMID: 39266501
  10. 组蛋白乳酸化作用驱动CD8+ T细胞的新陈代谢和功能。  |  Raychaudhuri, D., et al. 2024. Nat Immunol. 25: 2140-2151. PMID: 39375549

订购信息

产品名称产品编号规格价格数量收藏夹

CD8 抗体 (2.43)

sc-18860
200 µg/ml
RMB2377.00

CD8 抗体 (2.43) Alexa Fluor® 488

sc-18860 AF488
200 µg/ml
RMB2685.00

CD8 抗体 (2.43) Alexa Fluor® 647

sc-18860 AF647
200 µg/ml
RMB2685.00

CD8 抗体 (2.43) FITC

sc-18860 FITC
200 µg/ml
RMB2482.00

CD8 抗体 (2.43) PE

sc-18860 PE
200 µg/ml
RMB2580.00

Can CD8 (2.43): sc-18860 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD8 (2.43): sc-18860 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2018-07-11

Is this antibody suitable for CD8 detection in frozen tissue sections?

Asked by: HFerreira
Thank you for your question. Yes this antibody, sc-18860: CD8 (2.43), is recommended for detection of CD8 in frozen tissue sections of mouse origin, or IF in our product description, which is our abbreviation for immunohistochemistry in frozen tissue sections fixed in acetone, or immunofluorescent staining on methanol-fixed cells.
Answered by: Technical Support
Date published: 2016-09-19
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Rated 5 out of 5 by from Excellent!This antibody is recommended for detection of CD8 of mouse origin by IF and FCM
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent FCM data of CTLLExcellent FCM data of CTLL-2 cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Published data for WB in mouse CD8+ T cellsPublished data for WB in mouse CD8+ T cells. -SCBT Publication Review
Date published: 2015-05-14
Rated 5 out of 5 by from Published IHC and IF data for use in mousePublished IHC and IF data for use in mouse spleen and lymph node cells. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by from Exceptional immunofluorescence membrane stainingExceptional immunofluorescence membrane staining in methanol-fixed CTLL-2 cells. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Produced superb FCM data for mouse peripheralProduced superb FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-06-02
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CD8 抗体 (2.43) is rated 5.0 out of 5 by 6.
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