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CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) - Western Blotting - Image 15019
CD71 抗体 (YTA 74. 4): sc-59112. RAW 264. 7 (A), M1 (B) 和 TK-1 (C) 全细胞裂解液中 CD71 表达的 Western 印迹分析.
CD71/TFRC/Transferrin Receptor Antibody (YTA 74.4) - Western Blotting - Image 15019
CD71 抗体 (YTA 74. 4): sc-59112. RAW 264. 7 (A), M1 (B) 和 TK-1 (C) 全细胞裂解液中 CD71 表达的 Western 印迹分析.
CD71 抗体 (YTA 74. 4): sc-59112. RAW 264. 7 (A), M1 (B) 和 TK-1 (C) 全细胞裂解液中 CD71 表达的 Western 印迹分析.

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4): sc-59112

3.8(5)
寫評論提問

说明书
  • CD71/TFRC/Transferrin Receptor 抗体 YTA 74.4 是大鼠单克隆 IgG2a, 在7篇文献中引用，提供 200 µg/ml
  • 针对mouse来源的Con-A刺激脾细胞
  • 抗-CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) 推荐用于 WB, IP, IF 和 FCM，检测mouse 和rat 来源的 CD71
  • 抗CD71/TFRC/Transferrin Receptor抗体（YTA 74.4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD71/TFRC/Transferrin Receptor (YTA 74.4): sc-59112的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD71抗体（YTA 74.4）是一种鼠单克隆IgG2a抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）识别小鼠和大鼠来源的CD71蛋白。CD71 (YTA 74.4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CD71，也称为转铁蛋白受体（TFR），是一种II型膜糖蛋白，通过受体介导的内吞作用维持体内铁平衡。由于铁是许多酶的重要辅助因子，因此这一过程支持DNA合成和细胞增殖。CD71在增殖细胞、网状细胞和红细胞前体中表达量高，在免疫反应中活化的淋巴细胞、单核细胞和巨噬细胞中的表达量增加。CD71的表达扩展到大多数分裂细胞和脑内皮细胞，表明具有广泛的生理意义。第二种转铁蛋白受体TFR2也有助于转铁蛋白结合的铁的吸收，主要在肝脏，有助于铁的调节。TFR2基因突变可导致遗传性血色素沉着病III型（HFE3），这是一种常见于白种人的铁超负荷疾病，突出了CD71在铁代谢和相关疾病中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4)

sc-59112
200 µg/ml
RMB2377.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) AC

sc-59112 AC
500 µg/ml, 25% agarose
RMB3129.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) HRP

sc-59112 HRP
200 µg/ml
RMB2377.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) FITC

sc-59112 FITC
200 µg/ml
RMB2482.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) PE

sc-59112 PE
200 µg/ml
RMB2580.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 488

sc-59112 AF488
200 µg/ml
RMB2685.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 546

sc-59112 AF546
200 µg/ml
RMB2685.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 594

sc-59112 AF594
200 µg/ml
RMB2685.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 647

sc-59112 AF647
200 µg/ml
RMB2685.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 680

sc-59112 AF680
200 µg/ml
RMB2685.00

CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) Alexa Fluor® 790

sc-59112 AF790
200 µg/ml
RMB2685.00

Hi, is this antibody is specific to mouse only? do you have data to corroborate this?

Asked by: Jencilin
Thank you for your question. CD71 (YTA 74.4) antibody is recommended for mouse and rat samples.
Answered by: BlakeJ
Date published: 2023-07-24

Can CD71 (YTA 74.4): sc-59112 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD71 (YTA 74.4): sc-59112 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good for IFIt worked for IF in mouse cells (B cell line). 4% PFA fixation, blocking+permeabilisation, 1:25 antibody dilution
Date published: 2019-04-26
Rated 4 out of 5 by from CD71 in rat liver WCLConditions: 60 ug protein/lane, 7.5% gel, BME dye, no heat. Primary antibody, 1:1000 in 2.5% nonfat milk-TBST; incubated overnight at 4C. Secondary antibody, 1:10k in 2.5% nonfat milk-TBST; incubated for 1 hr at RT. Imaged using ChemiDoc imager system and femto substrate. Strong band just below 100 kDa. There is some smearing, but heating samples at 37C for 30min showed reduced smearing; further optimization may lead to a cleaner blot.
Date published: 2017-10-26
Rated 2 out of 5 by from A little weak band for mouse hepatocytes samples1:500 dilution, the signaling is a little weak.and higher background
Date published: 2017-08-09
Rated 3 out of 5 by from not for human cellsit is a anti-mouse antibody, and not suitable for human cells
Date published: 2017-07-20
Rated 5 out of 5 by from Strong bands in Western blot analysis ofStrong bands in Western blot analysis of CD71 expression in RAW 264.7, M1 and TK-1 whole cell lysates. -SCBT QC
Date published: 2014-10-20
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CD71/TFRC/Transferrin Receptor 抗体 (YTA 74.4) is rated 3.8 out of 5 by 5.
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