Date published: 2025-9-9
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CD68 Antibody (E-11) - Western Blotting - Image 58547
CD68 抗体 (E-11): sc-17832. 未转染 293T: sc-117752 (A), 转染人 CD68 的 293T: sc-175248 (B) 和 THP-1 (C) 全细胞裂解液中 CD68 表达的 Western 印迹分析.
CD68 抗体 (E-11): sc-17832. 未转染 293T: sc-117752 (A), 转染人 CD68 的 293T: sc-175248 (B) 和 THP-1 (C) 全细胞裂解液中 CD68 表达的 Western 印迹分析.

CD68 抗体 (E-11): sc-17832

4.5(8)
寫評論提問

说明书
  • CD68抗体(E-11)是小鼠单克隆IgG1 κ, 在39篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD68的氨基酸100-354
  • CD68 抗体 (E-11) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测human 来源的 CD68
  • 抗CD68抗体（E-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD68 (E-11): sc-17832的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD68 抗体 (E-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD68 抗体 (E-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD68抗体（E-11）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测人类CD68。抗CD68抗体（E-11）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD68是酸性、高度糖基化的溶酶体糖蛋白（LGP）家族的一员，该家族还包括lamp-1和lamp-2，并与小鼠抗原巨细胞素同源。CD68在免疫系统中起着至关重要的作用，主要作为巨噬细胞和其他髓细胞（对吞噬作用和清除细胞碎片至关重要）的标记物。CD68主要位于细胞质颗粒和各种非造血组织的细胞质中，例如肝、肾小管和肾小球，也会出现在巨噬细胞、单核细胞、中性粒细胞、嗜碱性粒细胞和大型淋巴细胞的表面，但程度较轻。这些免疫细胞上的CD68表达对于其免疫反应功能至关重要，有助于病原体识别和凋亡细胞的摄取，从而促进组织稳态和炎症消退。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD68 抗体 (E-11)

sc-17832
200 µg/ml
RMB2377.00

CD68 (E-11): m-IgG Fc BP-HRP 套装

sc-526576
200 µg Ab; 10 µg BP
RMB2662.00

CD68 (E-11): m-IgG1 BP-HRP 套装

sc-531949
200 µg Ab; 20 µg BP
RMB2662.00

CD68 抗体 (E-11) AC

sc-17832 AC
500 µg/ml, 25% agarose
RMB3129.00

CD68 抗体 (E-11) HRP

sc-17832 HRP
200 µg/ml
RMB2377.00

CD68 抗体 (E-11) FITC

sc-17832 FITC
200 µg/ml
RMB2482.00

CD68 抗体 (E-11) PE

sc-17832 PE
200 µg/ml
RMB2580.00

CD68 抗体 (E-11) Alexa Fluor® 488

sc-17832 AF488
200 µg/ml
RMB2685.00

CD68 抗体 (E-11) Alexa Fluor® 546

sc-17832 AF546
200 µg/ml
RMB2685.00

CD68 抗体 (E-11) Alexa Fluor® 594

sc-17832 AF594
200 µg/ml
RMB2685.00

CD68 抗体 (E-11) Alexa Fluor® 647

sc-17832 AF647
200 µg/ml
RMB2685.00

CD68 抗体 (E-11) Alexa Fluor® 680

sc-17832 AF680
200 µg/ml
RMB2685.00

CD68 抗体 (E-11) Alexa Fluor® 790

sc-17832 AF790
200 µg/ml
RMB2685.00

Does the antibody sc-17832 require antigen retrieval?

Asked by: sara
Thank you for your question. We recommend performing antigen retrieval via Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2024-07-24

Can CD68 (E-11): sc-17832 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD68 (E-11): sc-17832 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from High qualityI used this antibody for immunocytochemical application (DAB) with acceptable results
Date published: 2022-02-25
Rated 5 out of 5 by from goodWe use this antibody for Western blot, the blot are wonderful and got bands very clear.
Date published: 2017-05-10
Rated 5 out of 5 by from CD68 AntibodyCD68 Antibody (E-11) produced great clean signal in WB.
Date published: 2017-05-07
Rated 5 out of 5 by from Versatile antibodyExcellent results with CD68 Antibody (E-11)! Specific, clean and strong results by WB, IHCP, IF and FCM
Date published: 2017-01-25
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic and membrane staining in methanol-fixed thp-1 cells. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Robust size band obtained in THPRobust size band obtained in THP-1 whole cell lysate. -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Single strong band at correct mw in USingle strong band at correct mw in U-937 whole cell lysate. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Exceptional FCM data of PMAExceptional FCM data of PMA-stimulated human peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-10
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CD68 抗体 (E-11) is rated 4.5 out of 5 by 8.
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