Date published: 2025-9-9

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CD68 抗体 (3F103): sc-70761

4.5(13)
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说明书
  • CD68抗体(3F103)是小鼠单克隆IgG1 κ, 在50篇文献中引用,规格为200 µg/ml
  • 与human肺泡巨噬细胞的亚细胞部分杂交
  • 推荐用于 mouse, rat 和 human 来源的CD68 WB, IP, IF, IHC(P) 和 FCM检测
  • 关于如何获取CD68 (3F103): sc-70761免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CD68 抗体 (3F103) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD68 抗体 (3F103) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD68抗体(3F103)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠、大鼠和人类来源的CD68。抗CD68抗体(3F103)有非结合形式。CD68与鼠抗原巨细胞素同源,是酸性、高度糖基化的溶酶体糖蛋白(LGP)家族的一员,该家族还包括lamp-1和lamp-2。CD68在免疫系统中起着至关重要的作用,主要作为巨噬细胞和其他髓细胞的一种标记物,这些细胞对于抵御病原体和调节炎症至关重要。CD68主要位于细胞质颗粒和各种非造血组织的细胞质中,包括肝、肾小管和肾小球,也存在于巨噬细胞、单核细胞、中性粒细胞、嗜碱性粒细胞和大型淋巴细胞表面,但含量较少。这些免疫细胞上的CD68对于识别和定性至关重要,有助于了解它们在免疫反应和组织稳态中的作用。此外,溶酶体膜的主要成分是溶酶体糖蛋白,如CD68,它们有助于保护这些膜免受水解酶的降解,从而保持细胞的完整性和功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD68 抗体 (3F103)

sc-70761
200 µg/ml
RMB2377.00

CD68 (3F103): m-IgG Fc BP-HRP 套装

sc-528652
200 µg Ab; 10 µg BP
RMB2662.00

CD68 (3F103): m-IgGκ BP-HRP 套装

sc-521091
200 µg Ab, 40 µg BP
RMB2662.00

CD68 (3F103): m-IgG1 BP-HRP 套装

sc-543054
200 µg Ab; 20 µg BP
RMB2662.00

What concentration for Flow cytometry is proper to use? It is suggested in the datasheet to use 1 ug/million cells, however, what concentration of Ab is recommended?

Asked by: Anonymous
Thank you for your question. This antibody, CD68 antibody, sc-70761, is provided as 200µg in 1ml, so if you need to use 1µg you will need to take 5µl of antibody solution. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-11-19

Which is the volume of the vial? Because it is referenced as 200 µg/ml but recommended concentrations are not in µg/ml

Asked by: VíctorSrX
Thank you for your question. CD68 Antibody (3F103) is a mouse monoclonal IgG1 provided at 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2017-02-09

What is the best secondary to use with this antibody in Western Blot?

Asked by: Anna99
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Anyone knows which is the appropriate pH for Antigen Retrieval?

Asked by: VíctorSrX
Thank you for your question. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-01-11
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Rated 1 out of 5 by from Not good for WB; too faint even at 1:200THP1 macrophages and Raw macrophages showed very faint bands to none when loaded15- 80ug proteins/well for WB; used 1:200 at Room temp after repeated use of this antibody for 2 days in cold.
Date published: 2018-12-20
Rated 4 out of 5 by from Good ABThis antibody gave a good result in IHC on frozen human section in a dilution of 1:100.
Date published: 2018-04-03
Rated 5 out of 5 by from great for TAM detectingI am very glad to receive your prdoucts two weeks ago.we use this antibody to mark the monocytes cells isolated from the peripheral blood very well, which is very helpful to our research.
Date published: 2017-12-04
Rated 5 out of 5 by from CD 68 immunohistochemical staining for TAMsIt worked for our tumor tissue biopsy sample. I got excellent result in immunohistochemical staining by using this CD68 antibody for our tumor-associated macrophage study.
Date published: 2017-12-04
Rated 3 out of 5 by from for WB analysisThis antibody tried many times,at first no band.I tried different dilutions, different expose time,finally tried 1:200 and incubated 2hour at RT, however the band still not strong and the antibody conceration is a little low.
Date published: 2017-06-25
Rated 5 out of 5 by from Great CD68 antibodyCD68 (3F103) works great for WB and IF. Specific IF staining of methanol-fixed HeLa cells showing membrane localization.
Date published: 2017-01-11
Rated 5 out of 5 by from CD 68 immunohistochemical staining for SCLCIt worked for our FFPE format lymph node and lung tissue biopsy sample. We got excellent result in immunohistochemical staining by using this CD68 antibody for our macrophage phenotypic study.
Date published: 2016-12-13
Rated 5 out of 5 by from Highly recommendable antibody in IHC PHighly recommendable antibody in IHC P
Date published: 2015-12-07
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CD68 抗体 (3F103) is rated 4.5 out of 5 by 13.
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