Date published: 2025-9-6
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CD64 Antibody (10.1) - Western Blotting - Image 381599
CD64 抗体 (10. 1): sc-1184. 人 PBL 全细胞裂解液中 CD64 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.
CD64 抗体 (10. 1): sc-1184. 人 PBL 全细胞裂解液中 CD64 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.

CD64 抗体 (10.1): sc-1184

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寫評論提問

说明书
  • CD64 抗体 10.1 是小鼠单克隆 IgG1 κ，CD64抗体, 在23篇文献中引用，规格为200 µg/ml
  • 针对 human 类风湿滑膜液细胞/单核细胞
  • CD64 抗体 (10.1) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 CD64 cell surface marker
  • 抗CD64抗体（10.1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD64 (10.1): sc-1184的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是CD64 Antibody (10.1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD64 Antibody (10.1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD64抗体（10.1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类CD64蛋白。CD64 (10.1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CD64，也称为FCGR1A、FcγRI或高亲和力Fc-γ受体，是一种表面糖蛋白，通过高亲和力结合单体免疫球蛋白G（IgG）在免疫反应中发挥关键作用。CD64主要表达于单核细胞和巨噬细胞，在γ干扰素刺激下，中性粒细胞也会表达CD64。CD64是吞噬作用和细胞介导的细胞毒性的媒介，而吞噬作用和细胞介导的细胞毒性是清除病原体和调节免疫反应的重要过程。CD64已被证明可与多种信号分子相互作用，包括Src家族激酶，如Hck和Lyn，它们在受体交联时增强信号传导能力。这种相互作用激活免疫细胞和随后的免疫反应，凸显了CD64在先天免疫和适应性免疫中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD64 抗体 (10.1) 参考文献:

  1. 人类单核细胞中的新型 Fc γ 受体 I 家族基因产品。  |  Porges, AJ., et al. 1992. J Clin Invest. 90: 2102-9. PMID: 1430234
  2. 中性粒细胞的 CD64 表达。  |  Sack, U. 2017. Cytometry B Clin Cytom. 92: 189-191. PMID: 25522066
  3. 人类骨髓中的 CD66b-CD64dimCD115- 细胞代表了中性粒细胞的祖细胞。  |  Calzetti, F., et al. 2022. Nat Immunol. 23: 679-691. PMID: 35484408
  4. 表达 FcγR 融合体 CD64/16A 的 iPSC 衍生自然杀伤细胞可以用抗体武装起来，用于多肿瘤抗原靶细胞。  |  Snyder, KM., et al. 2023. J Immunother Cancer. 11: PMID: 38056893
  5. CD64 在糖尿病伤口愈合中发挥着关键作用。  |  Zhang, X., et al. 2024. Front Immunol. 15: 1322256. PMID: 38524127
  6. P75NTR+CD64+ 中性粒细胞可促进脓毒症诱发的急性肺损伤。  |  Fu, D., et al. 2024. Clin Immunol. 263: 110206. PMID: 38599263
  7. CD64+成纤维细胞靶向的维兰特罗和 STING 激动剂通过减少脱落细胞和富集干样 CD8+ T 细胞增强了 CLDN18.2 BiTEs 对胰腺癌的疗效。  |  Zhou, T., et al. 2024. Gut. 73: 1984-1998. PMID: 39187291
  8. 人类 T 淋巴细胞亚群中低亲和力 IgG 受体 Fc gamma RII（CD32）和 Fc gamma RIII（CD16）的活化依赖性表达。  |  Engelhardt, W., et al. 1995. Immunobiology. 192: 297-320. PMID: 7649565
  9. 在 IL-4 和 IFN-gamma 的作用下，IL-10 能上调人类单核细胞的吞噬作用。  |  Capsoni, F., et al. 1995. J Leukoc Biol. 58: 351-8. PMID: 7665991
  10. 人类 Fc gamma RII（CD32）的多个区域有助于与 IgG 结合。  |  Hulett, MD., et al. 1995. J Biol Chem. 270: 21188-94. PMID: 7673151
  11. 在粒细胞集落刺激因子治疗过程中，IgG 的高亲和力受体（Fc γ RI；CD64）参与增强了中性粒细胞的肿瘤细胞毒性。  |  Valerius, T., et al. 1993. Blood. 82: 931-9. PMID: 7687898
  12. 高亲和力免疫球蛋白 G 受体（Fc γ RI）与激酶 Hck 和 Lyn 的物理和功能缔合作用。  |  Wang, AV., et al. 1994. J Exp Med. 180: 1165-70. PMID: 8064233

订购信息

产品名称产品编号规格价格数量收藏夹

CD64 抗体 (10.1)

sc-1184
200 µg/ml
RMB2377.00

CD64 (10.1): m-IgG Fc BP-HRP 套装

sc-528125
200 µg Ab; 10 µg BP
RMB2662.00

CD64 (10.1): m-IgGκ BP-HRP 套装

sc-520428
200 µg Ab, 40 µg BP
RMB2662.00

CD64 (10.1): m-IgG1 BP-HRP 套装

sc-542776
200 µg Ab; 20 µg BP
RMB2662.00

CD64 抗体 (10.1) AC

sc-1184 AC
500 µg/ml, 25% agarose
RMB3129.00

CD64 抗体 (10.1) HRP

sc-1184 HRP
200 µg/ml
RMB2377.00

CD64 抗体 (10.1) FITC

sc-1184 FITC
200 µg/ml
RMB2482.00

CD64 抗体 (10.1) PE

sc-1184 PE
200 µg/ml
RMB2580.00

CD64 抗体 (10.1) Alexa Fluor® 488

sc-1184 AF488
200 µg/ml
RMB2685.00

CD64 抗体 (10.1) Alexa Fluor® 546

sc-1184 AF546
200 µg/ml
RMB2685.00

CD64 抗体 (10.1) Alexa Fluor® 594

sc-1184 AF594
200 µg/ml
RMB2685.00

CD64 抗体 (10.1) Alexa Fluor® 647

sc-1184 AF647
200 µg/ml
RMB2685.00

CD64 抗体 (10.1) Alexa Fluor® 680

sc-1184 AF680
200 µg/ml
RMB2685.00

CD64 抗体 (10.1) Alexa Fluor® 790

sc-1184 AF790
200 µg/ml
RMB2685.00

Esse anticorpo possui reatividade com CD64 de macaco cynomolgus?

Asked by: Marcelo Alves Pinto
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-02-09
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Rated 1 out of 5 by from Doesn't work on FFPE sectionsThe antibody doesn't work for IHC on FFPE. I’ve tried even tonsil and bone marrow. No reaction at all or nuclear staining if I concentrate the antibody.
Date published: 2021-09-24
Rated 4 out of 5 by from good antibody for IFI used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by from Antibody working normallyI used it （CD64 （10.1）） for detecting mouse liver by western blot （dilution 1:500）,and appeared a strong bland.
Date published: 2017-07-05
Rated 5 out of 5 by from Great overall detectionA great choice for CD64 detection. It stains and blots well and even works great for flow cytometry. This antibody is supported by more than ten citations.
Date published: 2017-01-20
Rated 4 out of 5 by from strong band at 50 kDThere was a strong band at 50 kD in human decidual macrophage samples by WB
Date published: 2016-12-14
Rated 5 out of 5 by from Extremely satisfied WB data in human liver tissue.Extremely satisfied WB data were detected in human liver tissue! Cooooooool!
Date published: 2016-11-25
Rated 5 out of 5 by from great productThis product is very easy to use. I will buy it again next time.
Date published: 2016-11-01
Rated 5 out of 5 by from 100 g/ml antibody used for IF with pfa fixed100 g/ml antibody used for IF with pfa fixed Raji lymphoid cells. -SCBT Publication Review
Date published: 2015-07-02
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CD64 抗体 (10.1) is rated 4.3 out of 5 by 12.
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