Date published: 2025-9-7

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CD59 抗体 (YTH53.1): sc-59095

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说明书
  • CD59 抗体 YTH53.1 是大鼠单克隆 IgG2b, 在12篇文献中引用,提供 200 µg/ml
  • 针对human来源的外周血T细胞
  • 抗-CD59 抗体 (YTH53.1) 推荐用于 WB, IP, IF 和 FCM,检测mouse, rat 和human 来源的 CD59
  • 抗CD59抗体(YTH53.1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD59 (YTH53.1): sc-59095免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成CD59 抗体 (YTH53.1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

CD59抗体(YTH53.1)是一种鼠单克隆IgG2b抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测小鼠、大鼠和人类来源的CD59蛋白。CD59抗体(YTH53.1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CD59是一种以糖基磷脂酰肌醇(GPI)为锚的糖蛋白,主要位于白细胞、血管内皮细胞、各种上皮细胞和胎盘表面,在免疫调节中起着至关重要的作用。CD59在细胞膜上的战略位置至关重要,因为CD59可以抵御补体系统,防止形成可导致细胞溶解的膜攻击复合物(MAC)。通过与MAC的C8和C9成分结合,CD59可有效抑制溶解孔的形成,从而保护宿主细胞免受补体介导的损害。此外,CD59还参与调节钙离子释放和激活脂筏相关信号分子,包括对各种细胞过程至关重要的酪氨酸激酶。CD59基因包含两个p53反应域,表明其在炎症反应中保护细胞免受补体诱导损伤方面具有潜在作用,并表明p53可能影响肿瘤细胞在化疗期间对补体裂解的敏感性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD59 抗体 (YTH53.1) 参考文献:

  1. 将GPI锚定的Ly6蛋白uPAR和CD59与病毒感染联系起来。  |  Yu, J., et al. 2019. Viruses. 11: PMID: 31739586
  2. 血型抗原CD59与疾病的关系。  |  Weinstock, C. 2022. Transfus Med Hemother. 49: 13-24. PMID: 35221864
  3. Cd59和炎症调节许旺细胞的发育  |  Wiltbank, AT., et al. 2022. Elife. 11: PMID: 35748863
  4. CD59调控的Ras区隔协调抗肿瘤T细胞免疫  |  Li, L., et al. 2022. Cancer Immunol Res. 10: 1475-1489. PMID: 36206575
  5. 抑制表皮生长因子受体上调的 CD55 和 CD59 表达可激活补体系统,并使肺癌对检查点阻断剂敏感。  |  Shao, F., et al. 2022. Nat Cancer. 3: 1192-1210. PMID: 36271172
  6. 骨骼肌中的CD59表达及其在重症肌无力中的作用。  |  Iwasa, K., et al. 2023. Neurol Neuroimmunol Neuroinflamm. 10: PMID: 36396448
  7. CD59抑制膜攻击复合物的结构基础。  |  Couves, EC., et al. 2023. Nat Commun. 14: 890. PMID: 36797260
  8. 遗传性CD59缺乏症,神经学和遗传学相互交织。  |  Almutawea, LM., et al. 2023. Neurosciences (Riyadh). 28: 130-135. PMID: 37045466
  9. 揭示 CD59 与抗体的相互作用,设计用于调控补体的仿 Paratope 肽。  |  Sandomenico, A., et al. 2023. Int J Mol Sci. 24: PMID: 37239905
  10. GPI锚定的CD59的动力学和分子相互作用。  |  Voisin, TB., et al. 2023. Toxins (Basel). 15: PMID: 37505699

订购信息

产品名称产品编号规格价格数量收藏夹

CD59 抗体 (YTH53.1)

sc-59095
200 µg/ml
RMB2377.00

CD59 抗体 (YTH53.1) AC

sc-59095 AC
500 µg/ml, 25% agarose
RMB3129.00

CD59 抗体 (YTH53.1) HRP

sc-59095 HRP
200 µg/ml
RMB2377.00

CD59 抗体 (YTH53.1) FITC

sc-59095 FITC
200 µg/ml
RMB2482.00

CD59 抗体 (YTH53.1) PE

sc-59095 PE
200 µg/ml
RMB2580.00

CD59 抗体 (YTH53.1) Alexa Fluor® 488

sc-59095 AF488
200 µg/ml
RMB2685.00

CD59 抗体 (YTH53.1) Alexa Fluor® 546

sc-59095 AF546
200 µg/ml
RMB2685.00

CD59 抗体 (YTH53.1) Alexa Fluor® 594

sc-59095 AF594
200 µg/ml
RMB2685.00

CD59 抗体 (YTH53.1) Alexa Fluor® 647

sc-59095 AF647
200 µg/ml
RMB2685.00

CD59 抗体 (YTH53.1) Alexa Fluor® 680

sc-59095 AF680
200 µg/ml
RMB2685.00

CD59 抗体 (YTH53.1) Alexa Fluor® 790

sc-59095 AF790
200 µg/ml
RMB2685.00

如何获得10ug小样?

Asked by: wangzihui
感谢您的提问。您可以拨打电话:021-60936350,发送邮件到[email protected],或者我们官网上的在线聊天,联系我们的亚洲技术支持团队,了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2022-10-31

Are there any issues with using sc-59095: CD59 (YTH53.1) mouse monoclonal primary antibody on mouse samples?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-59095 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Works for WB but not suitable for IPGives a signal in WB in 5%Milk/TBST (50 µg, HCT116 membrane fraction lysate). However, does not work in IP experiments.
Date published: 2020-10-01
Rated 5 out of 5 by from Works nicely in FCM assaysThe CD59 Antibody works nicely in my flow cytometry experiments
Date published: 2018-11-15
Rated 5 out of 5 by from Perfect antibody for CD59 detectionWe used this antibody for CD59 expression detection with western blot. We got a perfect band at the correct molecular weight for HeLa cells
Date published: 2017-01-31
Rated 5 out of 5 by from StrongStrong, clean band in western blot analysis of CD59 expression in ES-2, Caki-1, U-937, K-562 and THP-1 whole cell lysates. -SCBT QC
Date published: 2015-02-23
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CD59 抗体 (YTH53.1) is rated 4.5 out of 5 by 4.
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