Date published: 2025-9-7
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CD48 Antibody (HM48-1) - Western Blotting - Image 4888
CD48 抗体 (HM48-1): sc-19666. CTLL-2 (A), BW5147 (B) 和 I-11. 13 (C) 全细胞裂解液及小鼠胸腺组织提取物 (D) 中 CD48 表达的 Western 印迹分析.
CD48 抗体 (HM48-1): sc-19666. CTLL-2 (A), BW5147 (B) 和 I-11. 13 (C) 全细胞裂解液及小鼠胸腺组织提取物 (D) 中 CD48 表达的 Western 印迹分析.

CD48 抗体 (HM48-1): sc-19666

5.0(2)
寫評論提問

说明书
  • CD48 Antibody (HM48-1) is a Armenian hamster monoclonal IgG provided at 200 µg/ml
  • 推荐用于 mouse 和 rat 来源的CD48 WB, IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 目前，我们还没有完成CD48 抗体 (HM48-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD48是一种GPI锚定的糖蛋白，可以作为CD2的低亲和力受体。CD48在所有外周血T、B和空白细胞、胸腺细胞、嗜酸性粒细胞、骨髓细胞和一些上皮细胞上均表达。大鼠CD48可在造血细胞和内皮细胞上发现，而小鼠CD48可在胸腺细胞、淋巴结细胞、大多数巨噬细胞和骨髓细胞上发现。CD48已被证明与酪氨酸激酶Lck有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD48 抗体 (HM48-1) 参考文献:

  1. CD48在实验性结肠炎中控制T细胞和抗原呈递细胞的功能。  |  Abadía-Molina, AC., et al. 2006. Gastroenterology. 130: 424-34. PMID: 16472597
  2. CD2-CD48介导的膜间间距的纳米级增加可降低粘附力并重组免疫突触。  |  Milstein, O., et al. 2008. J Biol Chem. 283: 34414-22. PMID: 18826951
  3. CD48作为多发性骨髓瘤抗体治疗的新分子靶标。  |  Hosen, N., et al. 2012. Br J Haematol. 156: 213-24. PMID: 22098460
  4. 哮喘患者血液白细胞和血清中的CD48随严重程度而变化。  |  Gangwar, RS., et al. 2017. Allergy. 72: 888-895. PMID: 27859399
  5. 巨细胞病毒编码的可溶性CD48诱捕受体颠覆自然杀伤细胞反应。  |  Martínez-Vicente, P., et al. 2019. PLoS Pathog. 15: e1007658. PMID: 30947296
  6. 急性髓性白血病通过表观遗传学 CD48 沉默实现免疫逃逸。  |  Wang, Z., et al. 2020. Clin Sci (Lond). 134: 261-271. PMID: 31922199
  7. 全基因组CRISPR筛选确定CD48在外周T细胞淋巴瘤中决定对NK细胞毒性的敏感性。  |  Chiba, M., et al. 2022. Blood. 140: 1951-1963. PMID: 35921533
  8. CD48作为宫颈癌免疫微环境中的免疫相关预后标志物，可抑制增殖和迁移。  |  Ma, Y., et al. 2024. Carcinogenesis. 45: 57-68. PMID: 37279525
  9. 利用lncRNA NeST选择性增强YTS细胞，从而根除CD48阳性肿瘤。  |  Kotzur, R., et al. 2023. iScience. 26: 107284. PMID: 37609636
  10. 泛癌中CD48与肿瘤微环境相互作用的系统性免疫和预后分析。  |  He, M., et al. 2023. Int J Gen Med. 16: 5255-5269. PMID: 38021043

订购信息

产品名称产品编号规格价格数量收藏夹

CD48 抗体 (HM48-1)

sc-19666
200 µg/ml
RMB2377.00

CD48 抗体 (HM48-1) FITC

sc-19666 FITC
200 µg/ml
RMB2482.00

CD48 抗体 (HM48-1) PE

sc-19666 PE
200 µg/ml
RMB2580.00

For the CD48 (HM48-1): sc-19666 Western blot shown, were the lysates and tissue extracts run under reducing or non-reducing conditions?

Asked by: johneb
Thank you for your question. The Wester blot data on our website for this product, sc-19666, was using reducing de-naturing conditions. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-09-25

How can CD48 (HM48-1): sc-19666 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: cjMara
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-19666 PE) or fluoroscein (sc-19666 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent immunofluorescence membrane stainingExcellent immunofluorescence membrane staining in methanol-fixed CTLL-2 cells. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Produced excellent FCM data for mouse peripheralProduced excellent FCM data for mouse peripheral blood leukocytes. -SCBT QC
Date published: 2014-05-16
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CD48 抗体 (HM48-1) is rated 5.0 out of 5 by 2.
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