Date published: 2025-9-6

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CD45RA 抗体 (4KB5): sc-20057

5.0(4)
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说明书
  • CD45RA抗体(4KB5)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 针对human毛细胞白血病细胞的
  • 推荐用于 human 来源的CD45RA WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 CD45RA 抗体 (4KB5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD45RA 抗体 (4KB5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD45RA抗体(4KB5)是一种小鼠单克隆IgG1 kappa轻链抗体,专门针对CD45(PTPRC)的CD45RA同种型,该同种型在人类毛细胞白血病细胞中大量表达。CD45RA是一种跨膜糖蛋白,在调节造血细胞方面发挥着关键作用,其结构复杂,由保守的细胞内结构域和可变的细胞外结构域组成。胞外区域经历外显子4、5和6的替代剪接,形成多种同源物,如CD45RA、CD45RB、CD45RC和CD45RO,它们在外表位点方面各不相同,且仅在各种免疫细胞类型中分布。这种结构上的差异对于CD45RA(4KB5)单克隆抗体进行精确结合和识别至关重要,从而能够在蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)中实现精确检测和分析。CD45RA的保守细胞内结构域可作为磷酪氨酸磷酸酶发挥作用,对调节TCR/CD3复合物引发的酪氨酸磷酸化事件至关重要,从而影响Src和Syk家族激酶(如Lck、Fyn和ZAP-70)介导的关键信号通路。这种结构与功能的完整性使得CD45RA对于有效的免疫细胞活化和信号传导不可或缺,这凸显了抗CD45RA抗体(4KB5)对于研究免疫细胞生物学、信号传导机制和相关血液疾病的研究人员来说是一个至关重要的工具。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD45RA 抗体 (4KB5)

sc-20057
200 µg/ml
RMB2377.00

CD45RA (4KB5): m-IgG Fc BP-HRP 套装

sc-536648
200 µg Ab; 10 µg BP
RMB2662.00

CD45RA (4KB5): m-IgGκ BP-HRP 套装

sc-533806
200 µg Ab; 40 µg BP
RMB2662.00

CD45RA (4KB5): m-IgG1 BP-HRP 套装

sc-544732
200 µg Ab; 20 µg BP
RMB2662.00

CD45RA 抗体 (4KB5) APCC7

sc-20057 APCC7
100 tests in 2 ml
RMB2775.00

CD45RA 抗体 (4KB5) FITC

sc-20057 FITC
200 µg/ml
RMB2482.00

CD45RA 抗体 (4KB5) PE

sc-20057 PE
200 µg/ml
RMB2580.00

Can CD45RA (4KB5): sc-20057 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45RA (4KB5): sc-20057 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong band detected in NAMALWA and GA-10 whole cell lysates. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Good WB publishable data gathered with human U266Good WB publishable data gathered with human U266, ILKM2 and NOP2 cells. -SCBT Publication Review
Date published: 2015-03-26
Rated 5 out of 5 by from Superb immunoperoxidase membrane and cytoplasmicSuperb immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-01-12
Rated 5 out of 5 by from Produced superb FCM data for human peripheralProduced superb FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-08-21
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CD45RA 抗体 (4KB5) is rated 5.0 out of 5 by 4.
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