Date published: 2025-9-6
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CD45 Antibody (T29/33) - Western Blotting - Image 4846
CD45 抗体 (T29/33): sc-18901. Jurkat (A), CCRF-CEM (B) 和 HL-60 (C) 全细胞裂解液中 CD45 表达的 Western 印迹分析.
CD45 抗体 (T29/33): sc-18901. Jurkat (A), CCRF-CEM (B) 和 HL-60 (C) 全细胞裂解液中 CD45 表达的 Western 印迹分析.

CD45 抗体 (T29/33): sc-18901

4.5(6)
寫評論提問

说明书
  • CD45抗体(T29/33)是小鼠单克隆IgG1 κ, 在6篇文献中引用，规格为200 µg/ml
  • 针对human细胞系CCRF-CEM
  • 推荐用于 human 来源的CD45 WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD45 (35-Z6): sc-1178，可查看有标记的 CD45 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CD45 抗体 (T29/33) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD45 抗体 (T29/33) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

此外 CD45 抗体包括 CD45R, CD45RA, CD45RB, CD45RC 和 CD45RO

CD45已被鉴定为跨膜糖蛋白，在造血细胞中广泛表达。CD45的多种异构体根据细胞类型分布在整个免疫系统中。这些异构体是由于外显子4、5和6的可变剪接而产生的。相应的蛋白质结构域的特征在于特异性结合于CD45RA（外显子4）、CD45RB（外显子5）、CD45RC（外显子6）和CD45RO（外显子4至6被剪接掉）的单克隆抗体的结合。这些异构体的变化局限于CD45的细胞外结构域，而细胞内结构域是保守的。CD45具有磷酸酪氨酸磷酸酶的功能，是TCR/CD3复合物有效诱导酪氨酸磷酸化的重要组成部分。CD45的酪氨酸磷酸酶活性包含在保守的细胞内结构域中。TCR/CD3复合物利用Src和Syk家族蛋白酪氨酸激酶启动信号级联。这两个家族的几个成员，包括Lck、Fyn和ZAP-70，已被证实为CD45的生理底物。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (T29/33)

sc-18901
200 µg/ml
RMB2377.00

CD45 (T29/33): m-IgG Fc BP-HRP 套装

sc-536611
200 µg Ab; 10 µg BP
RMB2662.00

CD45 (T29/33): m-IgGκ BP-HRP 套装

sc-533767
200 µg Ab; 40 µg BP
RMB2662.00

CD45 (T29/33): m-IgG1 BP-HRP 套装

sc-544706
200 µg Ab; 20 µg BP
RMB2662.00

CD45 抗体 (T29/33) FITC

sc-18901 FITC
200 µg/ml
RMB2482.00

CD45 抗体 (T29/33) PE

sc-18901 PE
200 µg/ml
RMB2580.00

Can CD45 (T29/33): sc-18901 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (T29/33): sc-18901 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Using feline mature red blood cellsUsing feline mature red blood cells, was not able to obtain successful results using FCM. Used FITC and PE conjugated antibodies.
Date published: 2015-06-26
Rated 5 out of 5 by from Excellent FCM data of BJAB cellsExcellent FCM data of BJAB cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Bands at expected MW present in western blotBands at expected MW present in western blot analysis of CD45 expression in Jurkat, CCRF-CEM and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-04-28
Rated 5 out of 5 by from Beautiful immunofluorescence membrane stainingBeautiful immunofluorescence membrane staining in methanol-fixed Jurkat cells. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Successfully immunoprecipitated CD45 fromSuccessfully immunoprecipitated CD45 from human B cells and verified via WB. -SCBT Publication Review
Date published: 2015-01-31
Rated 5 out of 5 by from Excellent FCM data of Jurkat cellsExcellent FCM data of Jurkat cells. -SCBT QC
Date published: 2013-06-16
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CD45 抗体 (T29/33) is rated 4.5 out of 5 by 6.
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