Date published: 2025-9-6

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CD45 抗体 (MB4B4): sc-21739

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说明书
  • CD45 抗体 MB4B4 是大鼠单克隆 IgG, 在3篇文献中引用,提供 200 µg/ml
  • 抗免疫球蛋白激活的mouseB淋巴细胞
  • 推荐用于 mouse 来源的CD45 WB, IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD45 (35-Z6): sc-1178,可查看有标记的 CD45 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成CD45 抗体 (MB4B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

CD45已被鉴定为跨膜糖蛋白,在造血细胞中广泛表达。CD45的多种异构体根据细胞类型分布在整个免疫系统中。这些异构体的产生是由于外显子4、5和6的选择性剪接。相应的蛋白质结构域的特征在于特异性针对CD45RA(外显子4)、CD45RB(外显子5)、CD45RC(外显子6)和CD45RO(外显子4至6被剪接掉)的单克隆抗体的结合。这些异构体的变化局限于CD45的细胞外结构域,而细胞内结构域是保守的。CD45作为磷酸酪氨酸磷酸酶发挥作用,这是TCR/CD3复合物有效诱导酪氨酸磷酸化的重要组成部分。CD45的酪氨酸磷酸酶活性包含在其保守的细胞内结构域中。TCR/CD3复合物利用Src和Syk家族蛋白酪氨酸激酶来启动信号级联。这两个家族的几个成员,包括Lck、Fyn和ZAP-70,已被证实为CD45的生理底物。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (MB4B4)

sc-21739
200 µg/ml
RMB2377.00

CD45 抗体 (MB4B4) APCC7

sc-21739 APCC7
100 tests in 2 ml
RMB2775.00

CD45 抗体 (MB4B4) FITC

sc-21739 FITC
200 µg/ml
RMB2482.00

CD45 抗体 (MB4B4) PE

sc-21739 PE
200 µg/ml
RMB2580.00

Hello guys. Can we use CD45 (MB4B4) for flow cytometry detection in Human Clinical Samples? Does it come pre-conjugated with PE or APC or FITC or any other? If no conjugation is available how should we use the same in a clinical setting? Please reply asap

Asked by: Sumit Dey
Thank you for your question. Yes, this antibody is recommended for Flow Cytometry, however, we do not recommend nor warranty our products for clinical samples. This product is for research use only. We do not offer it conjugated with any of the conjugates, I would recommend another antibody against CD45 that does come conjugated: CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Alternatively, we can provide this mouse monoclonal antibody, CD45 conjugated with any of your desired conjugated, but this would be a special order. Please contact one of our Special Orders coordinators by email at [email protected] for more details.
Answered by: Tech Service
Date published: 2017-03-31

Can CD45 (MB4B4): sc-21739 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (MB4B4): sc-21739 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2023-08-01
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Rated 5 out of 5 by from Strong band in WB in mouse cells at 1:500 dilutionStrong band in WB in mouse cells at 1:500 dilution.
Date published: 2015-09-23
Rated 4 out of 5 by from Used for IP with CD4 T cellsUsed for IP with CD4 T cells. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by from Clean band observed at correct MW in westernClean band observed at correct MW in western blot analysis of CD45 expression in BYDP whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by from Successful pulldown of CD45 from murine TSuccessful pulldown of CD45 from murine T-cells and CD4 cells (also used to detect via immunoblot). -SCBT Publication Review
Date published: 2015-02-24
Rated 4 out of 5 by from Produced wonderful FCM data for C57BL/6 mouseProduced wonderful FCM data for C57BL/6 mouse splenocytes. -SCBT QC
Date published: 2013-05-19
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CD45 抗体 (MB4B4) is rated 4.2 out of 5 by 5.
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