Date published: 2026-10-24

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CD45 Antibody (MB4B4) - Flow Cytometry - Image 11445
CD45 (MB4B4) PE: sc-21739 PE. C57BL/6 小鼠脾细胞的 FCM 分析. 黑线直方图代表同型对照: 正常大鼠 IgG2a, sc-2872.
CD45 Antibody (MB4B4) - Flow Cytometry - Image 11445
CD45 (MB4B4) PE: sc-21739 PE. C57BL/6 小鼠脾细胞的 FCM 分析. 黑线直方图代表同型对照: 正常大鼠 IgG2a, sc-2872.
CD45 Antibody (MB4B4) - Western Blotting - Image 4849
CD45 (MB4B4): sc-21739. 对 BYDP 全细胞裂解液中 CD45 的表达进行 Western 印迹分析.
CD45 (MB4B4) PE: sc-21739 PE. C57BL/6 小鼠脾细胞的 FCM 分析. 黑线直方图代表同型对照: 正常大鼠 IgG2a, sc-2872.

CD45 抗体 (MB4B4): sc-21739

4.2(5)
寫評論提問
说明书
  • CD45 抗体 MB4B4 是大鼠单克隆 IgG, 在3篇文献中引用，提供 200 µg/ml
  • 抗免疫球蛋白激活的mouseB淋巴细胞
  • 推荐用于 mouse 来源的CD45 WB, IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD45 (35-Z6): sc-1178，可查看有标记的 CD45 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成CD45 抗体 (MB4B4)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

附加CD45抗体，包括CD45R, CD45RA, CD45RB, CD45RC和CD45RO

CD45已被鉴定为跨膜糖蛋白，在造血细胞中广泛表达。CD45的多种异构体根据细胞类型分布在整个免疫系统中。这些异构体的产生是由于外显子4、5和6的选择性剪接。相应的蛋白质结构域的特征在于特异性针对CD45RA（外显子4）、CD45RB（外显子5）、CD45RC（外显子6）和CD45RO（外显子4至6被剪接掉）的单克隆抗体的结合。这些异构体的变化局限于CD45的细胞外结构域，而细胞内结构域是保守的。CD45作为磷酸酪氨酸磷酸酶发挥作用，这是TCR/CD3复合物有效诱导酪氨酸磷酸化的重要组成部分。CD45的酪氨酸磷酸酶活性包含在其保守的细胞内结构域中。TCR/CD3复合物利用Src和Syk家族蛋白酪氨酸激酶来启动信号级联。这两个家族的几个成员，包括Lck、Fyn和ZAP-70，已被证实为CD45的生理底物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD45 抗体 (MB4B4) 参考文献:

  1. CD45对Syk阴性肥大细胞中ZAP70而非Syk的Fc epsilon RI信号转导至关重要。  |  Zhang, J. and Siraganian, RP. 1999. J Immunol. 163: 2508-16. PMID: 10452987
  2. CD45在大鼠尖顶细胞上的表达：参与促炎细胞因子的产生。  |  De Dios, I., et al. 2005. FEBS Lett. 579: 6355-60. PMID: 16263122
  3. 并膜楔负向调节 B 细胞中 CD45 的功能。  |  Hermiston, ML., et al. 2005. Immunity. 23: 635-47. PMID: 16356861
  4. 由不同配体-受体系统介导的肥大细胞反应微调需要 CD45。  |  Grochowy, G., et al. 2009. Cell Signal. 21: 1277-86. PMID: 19332117
  5. CD45调节移植免疫缺陷小鼠中未成熟正常和白血病人类细胞的归巢和植入。  |  Shivtiel, S., et al. 2011. Exp Hematol. 39: 1161-1170.e1. PMID: 21911094
  6. 先天免疫细胞CD45调节淋巴细胞减少引起的T细胞增殖。  |  Saunders, AE., et al. 2014. J Immunol. 193: 2831-42. PMID: 25114101
  7. 利用免疫组化辅助 LA-ICP-MS 对转移性乳腺癌组织中的 MMP-11 和 CD45 进行定量多重分析。  |  Johnson, D., et al. 2022. Metallomics. 14: PMID: 35867868
  8. CD45同种型在人类造血细胞不同发育阶段中的表达。  |  Craig, W., et al. 1994. Br J Haematol. 88: 24-30. PMID: 7803253
  9. CD45 参与诱导 L 选择素下调  |  Stibenz, D., et al. 1996. Scand J Immunol. 44: 37-44. PMID: 8693290
  10. 参与T细胞受体信号传导的CD45-AP和蛋白酪氨酸激酶之间的相互作用。  |  Motoya, S., et al. 1999. J Biol Chem. 274: 1407-14. PMID: 9880514

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (MB4B4)

sc-21739
200 µg/ml
CNY2422.00

CD45 抗体 (MB4B4) APCC7

sc-21739 APCC7
100 tests in 2 ml
CNY2828.00

CD45 抗体 (MB4B4) FITC

sc-21739 FITC
200 µg/ml
CNY2527.00

CD45 抗体 (MB4B4) PE

sc-21739 PE
200 µg/ml
CNY2625.00

Hello guys. Can we use CD45 (MB4B4) for flow cytometry detection in Human Clinical Samples? Does it come pre-conjugated with PE or APC or FITC or any other? If no conjugation is available how should we use the same in a clinical setting? Please reply asap

Asked by: Sumit Dey
Thank you for your question. Yes, this antibody is recommended for Flow Cytometry, however, we do not recommend nor warranty our products for clinical samples. This product is for research use only. We do not offer it conjugated with any of the conjugates, I would recommend another antibody against CD45 that does come conjugated: CD45 (35-Z6): sc-1178 for CD45 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. Alternatively, we can provide this mouse monoclonal antibody, CD45 conjugated with any of your desired conjugated, but this would be a special order. Please contact one of our Special Orders coordinators by email at [email protected] for more details.
Answered by: Tech Service
Date published: 2017-03-31

Can CD45 (MB4B4): sc-21739 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (MB4B4): sc-21739 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2023-08-01
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Rated 5 out of 5 by M_R from Strong band in WB in mouse cells at 1:500 dilutionStrong band in WB in mouse cells at 1:500 dilution.
Date published: 2015-09-23
Rated 4 out of 5 by CJ from Used for IP with CD4 T cellsUsed for IP with CD4 T cells. -SCBT QC
Date published: 2015-05-17
Rated 4 out of 5 by JT from Clean band observed at correct MW in westernClean band observed at correct MW in western blot analysis of CD45 expression in BYDP whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 4 out of 5 by NZ from Successful pulldown of CD45 from murine TSuccessful pulldown of CD45 from murine T-cells and CD4 cells (also used to detect via immunoblot). -SCBT Publication Review
Date published: 2015-02-24
Rated 4 out of 5 by FL from Produced wonderful FCM data for C57BL/6 mouseProduced wonderful FCM data for C57BL/6 mouse splenocytes. -SCBT QC
Date published: 2013-05-19
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CD45 抗体 (MB4B4) is rated 4.2 out of 5 by 5.
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