Date published: 2025-9-6
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CD45 Antibody (30-F11) - Western Blotting - Image 376796
CD45 抗体 (30-F11): sc-53665. 小鼠胸腺组织提取物中 CD45 表达的 Western 印迹分析.
CD45 抗体 (30-F11): sc-53665. 小鼠胸腺组织提取物中 CD45 表达的 Western 印迹分析.

CD45 抗体 (30-F11): sc-53665

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说明书
  • CD45 抗体 30-F11 是大鼠单克隆 IgG2b, 在29篇文献中引用，提供 200 µg/ml
  • 针对mouse来源的胸腺和脾脏
  • CD45 抗体 (30-F11) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测mouse 来源的 CD45
  • 抗CD45抗体（30-F11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD45 (30-F11): sc-53665的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD45 抗体 (30-F11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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基因信息
说明书与实验方案
研究信息

関連項目

此外 CD45 抗体包括 CD45R, CD45RA, CD45RB, CD45RC 和 CD45RO

CD45抗体（30-F11）是一种IgG2b大鼠单克隆CD45抗体（也称为CD45抗体或PTPRC抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（IHC（P））和流式细胞术检测小鼠来源的CD45。CD45抗体（30-F11）既有非偶联型抗CD45抗体形式，也有多种偶联型抗CD45抗体形式，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。CD45已被确定为一种跨膜糖蛋白，在造血细胞中广泛表达。CD45的多种异构体根据细胞类型分布在整个免疫系统中。这些异构体的产生是由于外显子4、5和6的选择性剪接。相应的蛋白质结构域的特征在于特异性结合针对CD45RA（外显子4）、CD45RB（外显子5）、CD45RC（外显子6）和CD45RO（外显子4至6被剪接掉）的单克隆抗体。这些异构体的变异位于CD45的细胞外结构域，而细胞内结构域是保守的。CD45作为磷酸酪氨酸磷酸酶发挥作用，这是TCR/CD3复合物有效诱导酪氨酸磷酸化的关键组分。CD45的酪氨酸磷酸酶活性包含在保守的细胞内结构域内。TCR/CD3复合物利用Src和Syk家族蛋白酪氨酸激酶启动信号级联。这两个家族的几个成员，包括Lck、Fyn和ZAP-70，已被确定为CD45的生理底物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (30-F11)

sc-53665
200 µg/ml
RMB2377.00

CD45 抗体 (30-F11) AC

sc-53665 AC
500 µg/ml, 25% agarose
RMB3129.00

CD45 抗体 (30-F11) HRP

sc-53665 HRP
200 µg/ml
RMB2377.00

CD45 抗体 (30-F11) FITC

sc-53665 FITC
200 µg/ml
RMB2482.00

CD45 抗体 (30-F11) PE

sc-53665 PE
200 µg/ml
RMB2580.00

CD45 抗体 (30-F11) Alexa Fluor® 488

sc-53665 AF488
200 µg/ml
RMB2685.00

CD45 抗体 (30-F11) Alexa Fluor® 546

sc-53665 AF546
200 µg/ml
RMB2685.00

CD45 抗体 (30-F11) Alexa Fluor® 594

sc-53665 AF594
200 µg/ml
RMB2685.00

CD45 抗体 (30-F11) Alexa Fluor® 647

sc-53665 AF647
200 µg/ml
RMB2685.00

CD45 抗体 (30-F11) Alexa Fluor® 680

sc-53665 AF680
200 µg/ml
RMB2685.00

CD45 抗体 (30-F11) Alexa Fluor® 790

sc-53665 AF790
200 µg/ml
RMB2685.00

Do you have a product I can use as an isotype for the CD45 Antibody (30-F11) Alexa Fluor® 790 (a Rat IgG2b-AF790)? Thanks for your answer!

Asked by: Teresa_G
Thanks for your question. Unfortunately, at the moment we don´t offer a conjugated isotype from the requested species. Please contact technical service if you need any other information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-08
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Rated 5 out of 5 by from 抗体不错我们实验室之前经常买这个公司的抗体,最近也购买了一些，打算申请一个CD45de 试用装，好的话再买它
Date published: 2019-09-21
Rated 5 out of 5 by from Excellent antibodyI bought this about a month ago, I have used it for paraffin embedded samples of mice oral tissues and the leukocytes were very clear.
Date published: 2019-08-27
Rated 4 out of 5 by from 功能不错试用了小样，也购买了正式装，做流式和免疫荧光是一般调到最高稀释浓度才能得到结果，虽然效价似乎不高，但是贵在能够用在多种实验和种属，保存也方便
Date published: 2018-02-06
Rated 4 out of 5 by from 功能较多样化一个月前买的，可以用在多种实验中，种属适应范围刚好打到我们的实验要求，虽然实验中需要的浓度略高，但是效果还可以
Date published: 2018-02-06
Rated 5 out of 5 by from good wb resultThe antibody react with the cd45 in human leukocyte cell,it shows positive expression , I observed the specific band by dilution 1:1000.
Date published: 2017-07-08
Rated 5 out of 5 by from Good choice for FCMThis rat monoclonal, CD45 Antibody (30-F11), performs well for detection of mouse CD45 in flow cytometry applications and is readily available conjugated to PE and FITC.
Date published: 2017-01-26
Rated 3 out of 5 by from Faint band observed at correct MW in westernFaint band observed at correct MW in western blot analysis of CD45 expression in mouse PBL whole cell lysate. -SCBT QC
Date published: 2015-01-30
Rated 4 out of 5 by from Clear Indirect FCM data of mouse peripheralClear Indirect FCM data of mouse peripheral blood leukocytes stained with CD45 . -SCBT QC
Date published: 2014-11-20
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CD45 抗体 (30-F11) is rated 4.4 out of 5 by 8.
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