Date published: 2025-9-6
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CD45 Antibody (2D-1) - Western Blotting - Image 352437
CD45 (2D-1): sc-1187. CCRF-CEM (A), BJAB (B), GA-10 (C), Ramos (D) 和 HL-60 (E) 全细胞裂解液中 CD45 表达的 Western 印迹分析.
CD45 (2D-1): sc-1187. CCRF-CEM (A), BJAB (B), GA-10 (C), Ramos (D) 和 HL-60 (E) 全细胞裂解液中 CD45 表达的 Western 印迹分析.

CD45 抗体 (2D-1): sc-1187

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说明书
  • CD45抗体(2D-1)是小鼠单克隆IgG1 κ, 在22篇文献中引用，规格为200 µg/ml
  • 抗-CD45 抗体 (2D-1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 CD45, CD45RO, CD45A, CD45B and all forms of LCA
  • 抗CD45抗体（2D-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联APC-Cy7用于IF，IHC(P) 和 FCM
  • 关于如何获取CD45 (2D-1): sc-1187的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD45 抗体 (2D-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD45 抗体 (2D-1) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD45 抗体包括 CD45R, CD45RA, CD45RB, CD45RC 和 CD45RO

CD45抗体（2D-1）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测小鼠、大鼠和人类样本中的CD45（也称为蛋白酪氨酸磷酸酶受体C型（PTPRC）），检测方法包括蛋白质印迹（WB）、免疫沉淀（IP）、PAR检测和流式细胞术（FCM）。CD45单克隆抗体（2D-1）靶向人类CD45的胞外结构域。CD45是一种重要的跨膜糖蛋白，在所有造血细胞表面都有显著表达，在调节免疫细胞信号和激活方面发挥着关键作用。CD45位于细胞膜上，通过去磷酸化Src和Syk家族激酶（如Lck、Fyn和ZAP-70）上的特定酪氨酸残基，作为T和B细胞受体信号的关键调节因子。这种调节对于正常的免疫反应至关重要，包括T细胞的激活、增殖和分化，以及B细胞发育和抗体产生。CD45的胞外结构域可进行选择性剪接，形成多种异构体，从而根据不同细胞类型和激活状态对免疫功能进行微调。CD45在细胞表面的精确定位有助于CD45与其他膜结合受体和信号分子相互作用，因此CD45对于维持免疫平衡和应对病原体必不可少。CD45的失调或表达改变与多种免疫疾病有关，包括自身免疫性疾病和白血病，这凸显了CD45在正常免疫功能和疾病发病机理中的重要性。抗CD45抗体（2D-1）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和Alexa Fluor®结合物，为各种实验应用提供了多种选择。研究人员利用CD45单克隆抗体（2D-1）可以有效地研究CD45的表达、同型异构体的分布和功能动态，从而增进我们对免疫细胞生物学和相关疾病机制的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD45 抗体 (2D-1) 参考文献:

  1. CD45酪氨酸磷酸酶：免疫细胞功能的正负调节因子。  |  Alexander, DR. 2000. Semin Immunol. 12: 349-59. PMID: 10995582
  2. 对跨膜蛋白酪氨酸磷酸酶CD45的新见解。  |  Sasaki, T., et al. 2001. Int J Biochem Cell Biol. 33: 1041-6. PMID: 11551820
  3. 基于同系CD45层次结构的骨髓瘤细胞生长和成熟细胞模型。  |  Bataille, R., et al. 2003. Immunol Rev. 194: 105-11. PMID: 12846811
  4. 淋巴细胞上CD45抗体介导的交联诱导的细胞死亡途径。  |  Steff, AM., et al. 2003. Crit Rev Immunol. 23: 421-40. PMID: 15030310
  5. 病毒干扰细胞受体酪氨酸磷酸酶CD45的功能。  |  Thiel, N., et al. 2015. Viruses. 7: 1540-57. PMID: 25807057
  6. 在颈动脉结扎后，HIF诱导的CD45介导部分内皮细胞向间充质细胞转化，形成新内膜。  |  Yamashiro, Y., et al. 2023. Cardiovasc Res. 119: 1606-1618. PMID: 36537041
  7. 非造血细胞中CD45的表达：在再生医学和疾病管理中的应用。  |  Das, M., et al. 2024. Regen Med. 19: 407-419. PMID: 39058408
  8. CD45：作为淋巴细胞活化和发育所需的蛋白酪氨酸磷酸酶的新角色。  |  Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300
  9. 造血细胞中的白细胞共同抗原、CD45和其他蛋白酪氨酸磷酸酶。  |  Woodford-Thomas, T. and Thomas, ML. 1993. Semin Cell Biol. 4: 409-18. PMID: 8305680
  10. CD45相关蛋白是一种淋巴细胞特异性膜蛋白，以两种不同的形式表达。  |  Kitamura, K., et al. 1997. Eur J Immunol. 27: 383-8. PMID: 9045908

订购信息

产品名称产品编号规格价格数量收藏夹

CD45 抗体 (2D-1)

sc-1187
200 µg/ml
$316.00

CD45 (2D-1): m-IgG Fc BP-HRP 套装

sc-526466
200 µg Ab; 10 µg BP
$354.00

CD45 (2D-1): m-IgG1 BP-HRP 套装

sc-531839
200 µg Ab; 20 µg BP
$354.00

CD45 抗体 (2D-1) AC

sc-1187 AC
500 µg/ml, 25% agarose
$416.00

CD45 抗体 (2D-1) HRP

sc-1187 HRP
200 µg/ml
$316.00

CD45 抗体 (2D-1) FITC

sc-1187 FITC
200 µg/ml
$330.00

CD45 抗体 (2D-1) PE

sc-1187 PE
200 µg/ml
$343.00

CD45 抗体 (2D-1) Alexa Fluor® 488

sc-1187 AF488
200 µg/ml
$357.00

CD45 抗体 (2D-1) Alexa Fluor® 546

sc-1187 AF546
200 µg/ml
$357.00

CD45 抗体 (2D-1) Alexa Fluor® 594

sc-1187 AF594
200 µg/ml
$357.00

CD45 抗体 (2D-1) Alexa Fluor® 647

sc-1187 AF647
200 µg/ml
$357.00

CD45 抗体 (2D-1) Alexa Fluor® 680

sc-1187 AF680
200 µg/ml
$357.00

CD45 抗体 (2D-1) Alexa Fluor® 790

sc-1187 AF790
200 µg/ml
$357.00

CD45 抗体 (2D-1) APC-Cy7) APCC7

sc-1187 APCC7
100 tests in 2 ml
$369.00

Hello there. Do you guys do international delivery? I need CD45 Antibody (2D-1): sc-1187 (Saint Petersburg, Russia). What is your price if you do international delivery? How long it takes? And how long will take delivery to San Diego, CA (and price)?

Asked by: Nina Redfield
Thank you for your question. Please contact our Customer Care Department for further assistance.
Answered by: Technical Support
Date published: 2019-06-16

Is there any cd45 antibody can use in hamster?

Asked by: kaola2hao
Thank you for your question. Unfortunately, none of our CD45 antibodies are currently recommended for use in hamster, nor are we aware of any product citations using the CD45 antibodies in hamster. This monoclonal antibody, CD45 Antibody (B-8): sc-28369, is raised against a recombinant protein (amino acids 1075-1304 of CD45 of human origin) which shares 75% sequence identity with this published model hamster CD45 protein sequence, (accession number: XP_012971285). The exact epitope recognized by this monoclonal antibody has not been determined, however, based on this alignment there is a chance that the antibody may cross-react with hamster. However, because the alignment is not 100%, we still can not guarantee it.
Answered by: Technical Support
Date published: 2019-01-15

Can CD45 (2D-1): sc-1187 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD45 (2D-1): sc-1187 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from not good detection of mouse CD45 cellsmouse spleen staining shows less than 5% of positive cells.
Date published: 2018-05-12
Rated 4 out of 5 by from good antibodyI got a free sample from the company a month ago, and it worked for my IHC-p slides and no background staining, etc.. The only reason why I am not giving a 5-stars is because the intensity of the staining was not very strong. I was trying to stain lymphocytes infiltration in human lung sections.
Date published: 2017-11-22
Rated 5 out of 5 by from Excellent antibody!The CD45 Antibody (2D-1): sc-1187 provides great data for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited several times for further verification.
Date published: 2017-01-18
Rated 5 out of 5 by from Superb FCM data of human peripheral bloodSuperb FCM data of human peripheral blood leukocytes. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase membraneProduced excellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Clean western blot using 293T cell lysatesClean western blot using 293T cell lysates. -SCBT Publication Review
Date published: 2015-03-03
Rated 5 out of 5 by from Published WB and IF data with Jurkat T cellsPublished WB and IF data with Jurkat T cells, K592 and primary T cells. -SCBT Publication Review
Date published: 2015-01-28
Rated 5 out of 5 by from Excellent Indirect FCM data of HuT 78 cellsExcellent Indirect FCM data of HuT 78 cells stained with CD45 . -SCBT QC
Date published: 2013-07-03
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CD45 抗体 (2D-1) is rated 4.4 out of 5 by 8.
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