Date published: 2025-9-7
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CD4 Antibody (RM4-5) - Western Blotting - Image 51425
CD4 抗体 (RM4-5): sc-19643. 非转染: sc-117752 (A) 和小鼠 CD4 转染: sc-119107 (B) 293T 全细胞裂解液中 CD4 表达的 Western 印迹分析.
CD4 Antibody (RM4-5) - Western Blotting - Image 51425
CD4 抗体 (RM4-5): sc-19643. 非转染: sc-117752 (A) 和小鼠 CD4 转染: sc-119107 (B) 293T 全细胞裂解液中 CD4 表达的 Western 印迹分析.
CD4 Antibody (RM4-5) - Flow Cytometry - Image 5738
CD4 抗体 (RM4-5) PE: sc-19643 PE. 小鼠外周血白细胞的 FCM 分析. 象限标记根据同型对照正常大鼠 IgG2a: sc-2872 设置.
CD4 抗体 (RM4-5): sc-19643. 非转染: sc-117752 (A) 和小鼠 CD4 转染: sc-119107 (B) 293T 全细胞裂解液中 CD4 表达的 Western 印迹分析.

CD4 抗体 (RM4-5): sc-19643

3.8(4)
寫評論提問

说明书
  • CD4 抗体 RM4-5 是大鼠单克隆 IgG2a, 在7篇文献中引用，提供 200 µg/ml
  • 针对BALB/c mouse胸腺细胞
  • 推荐用于 mouse 来源的CD4 WB, IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD4 (MT310): sc-19641，可查看有标记的 CD4 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前，我们还没有完成CD4 抗体 (RM4-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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说明书与实验方案
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関連項目

T细胞受体（TCR）是一种由α和β或γ和δ链组成的异二聚体。CD3链和CD4或CD8共受体也是TCR有效信号转导的必要条件。TCR表达于T辅助细胞和T细胞毒性细胞，可以通过它们的CD4和CD8表达来区分。T辅助细胞表达CD4蛋白，T细胞毒性细胞显示CD8。CD4也表达于皮层细胞、成熟的髓样淋巴细胞、微样细胞和树突状细胞。CD4（也称为T4和Leu 3）是一种膜糖蛋白，具有四个细胞外免疫球蛋白样域。TCR与CD4联合可以结合抗原呈递细胞呈递的II型MHC分子。CD4蛋白的功能是增加TCR与抗原-II型MHC复合物之间相互作用的粘性。CD4的另一个作用是作为HIV的受体。

仅限研究使用。不适用于诊断和治疗用途。

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产品名称产品编号规格价格数量收藏夹

CD4 抗体 (RM4-5)

sc-19643
200 µg/ml
RMB2377.00

CD4 抗体 (RM4-5) FITC

sc-19643 FITC
200 µg/ml
RMB2482.00

CD4 抗体 (RM4-5) PE

sc-19643 PE
200 µg/ml
RMB2580.00

Can CD4 (RM4-5): sc-19643 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD4 (RM4-5): sc-19643 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good publishable data in IHC in mouse spleenGood publishable data in IHC in mouse spleen and lymph node. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Robust signal in WB in mouse transfectedRobust signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-14
Rated 4 out of 5 by from Published from flow in mouse cryostat KC cellsPublished from flow in mouse cryostat KC cells. -SCBT Publication Review
Date published: 2015-01-09
Rated 3 out of 5 by from Decent FCM data of mouse peripheral bloodDecent FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-03
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CD4 抗体 (RM4-5) is rated 3.8 out of 5 by 4.
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