Date published: 2025-9-7

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CD4 抗体 (H129.19): sc-19642

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说明书
  • CD4 抗体 H129.19 是大鼠单克隆 IgG2a, 在4篇文献中引用,提供 200 µg/ml
  • 针对A.TH. mouse CTL克隆A15.1.17
  • 推荐用于 mouse 来源的CD4 IP, IF 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 还可偶联APC 用于IF, IHC(P) 和FCM
  • 还提供无叠氮化物的阻断剂,用于阻断T辅助细胞对抗原或促有丝分裂原刺激的应答,sc-19642 L,200 µg/0.1 ml
  • 看 CD4 (MT310): sc-19641,可查看有标记的 CD4 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成CD4 抗体 (H129.19)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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CD4抗体(H129.19)是一种鼠单克隆IgG2a抗体,经过改造后能够识别CD4,也称为T4或Leu 3,特别适用于小鼠样本。抗CD4抗体(H129.19)针对A.TH.小鼠CTL克隆A15.1.17表位产生,推荐用于免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)应用,确保在各种实验条件下精确检测CD4。CD4是一种膜结合型糖蛋白,由四个类免疫球蛋白细胞外结构域组成,在T细胞活化中作为共受体发挥关键作用,通过增强T细胞受体(TCR)与抗原呈递细胞上的II类主要组织相容性复合体(MHC)分子之间的相互作用。翻译后修饰(如糖基化)在维持CD4的稳定性、细胞表面的正确定位以及与其他蛋白质的相互作用方面发挥着至关重要的作用,从而影响免疫反应的有效性。此外,CD4还是人体免疫缺陷病毒(HIV)的主要受体,因此CD4成为病毒入侵和免疫系统调节研究的重要目标。CD4在辅助性T细胞、皮质细胞、成熟髓质胸腺细胞、小胶质细胞和树突状细胞中的战略性定位凸显了CD4在适应性免疫和先天免疫中的重要性。CD4单克隆抗体(H129.19)能够准确识别和鉴定CD4阳性细胞,为免疫学领域的关键研究提供支持,包括自身免疫性疾病、传染性疾病和免疫疗法开发的研究。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

CD4 抗体 (H129.19)

sc-19642
200 µg/ml
RMB2377.00

CD4 抗体 (H129.19) APC

sc-19642 APC
100 tests in 2 ml
RMB2640.00

CD4 抗体 (H129.19) APCC7

sc-19642 APCC7
100 tests in 2 ml
RMB2775.00

CD4 抗体 (H129.19) FITC

sc-19642 FITC
200 µg/ml
RMB2482.00

CD4 抗体 (H129.19) L

sc-19642 L
200 µg/0.1 ml
RMB2377.00

CD4 抗体 (H129.19) PE

sc-19642 PE
200 µg/ml
RMB2580.00

Does this antibody work in IHC of sections from paraffin-embedded samples? Thanks for your help, Staffan

Asked by: Staffan
Thank you for your question. For staining paraffin-embedded, formalin-fixed tissue sample sections we would rather recommend using one of the two following alternate CD4 antibodies. We have several pieces of data and product citations for these products in the entiond application IHC(P). CD4 (MT310): sc-19641 https://www.scbt.com/scbt/product/cd4-antibody-mt310 CD4 (GK1.5): sc-13573 https://www.scbt.com/scbt/product/cd4-antibody-gk1-5 We suggest using the directly -HRP conjugated primary antibody according to our protocols: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining if you have any futher questions, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

Does H129-19 work in IHC for sections from paraffin-imbedded samples? Staffan

Asked by: Staffan
Thank you for your question. For staining paraffin-embedded, formalin-fixed tissue sample sections we would rather recommend using one of the two following alternate CD4 antibodies. We have several pieces of data and product citations for these products in the entiond application IHC(P). CD4 (MT310): sc-19641 https://www.scbt.com/scbt/product/cd4-antibody-mt310 CD4 (GK1.5): sc-13573 https://www.scbt.com/scbt/product/cd4-antibody-gk1-5 We suggest using the directly -HRP conjugated primary antibody according to our protocols: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining if you have any futher questions, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

How can CD4 (H129.19): sc-19642 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-19642 PE) or fluoroscein (sc-19642 FITC).
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Wonderful FCM analysis of mouse peripheralWonderful FCM analysis of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2013-09-11
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CD4 抗体 (H129.19) is rated 4.0 out of 5 by 1.
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