Date published: 2025-9-7
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CD36 Antibody (SMφ) - Western Blotting - Image 4813
CD36 (SMf): sc-7309. 对 HUV-EC-C 全细胞裂解液中 CD36 的表达进行 Western 印迹分析.
CD36 (SMf): sc-7309. 对 HUV-EC-C 全细胞裂解液中 CD36 的表达进行 Western 印迹分析.

CD36 抗体 (SMφ): sc-7309

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说明书
  • CD36 抗体 SMφ 是小鼠单克隆 IgM κ，CD36抗体, 在101篇文献中引用，规格为200 µg/ml
  • 针对human扁桃体细胞和周围血单核细胞
  • 推荐用于 mouse, rat 和 human 来源的CD36 WB, IP, IF, IHC(P) 和 FCM检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • CD36抗体（SMφ）可与Alexa Fluor® 594结合，用于IF、IHC(P)和FCM
  • 关于如何获取CD36 (SMφ): sc-7309的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD36 抗体 (SMφ)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD36抗体（SMφ）是一种小鼠单克隆IgM kappa轻链抗体，专门用于识别CD36，也称为血小板糖蛋白4（GPIV）、脂肪酸转运蛋白（FAT）、糖蛋白IIIb（GPIIIB）、清除受体B类成员3（SCARB3）和PAS IV。CD36是一种多功能膜糖蛋白，主要位于血小板、单核细胞和脐静脉内皮细胞表面。这种重要的细胞定位使CD36能够与多种配体相互作用，包括胶原蛋白、血栓素、阴离子磷脂和氧化低密度脂蛋白（LDL），从而促进吞噬作用和脂质代谢等关键过程。CD36介导的相互作用对于维持细胞内环境平衡、支持成熟精子的持续生成以及调节炎症反应至关重要。CD36在细胞粘附中发挥着重要作用，影响对组织完整性和修复至关重要的粘附过程。CD36的突变可导致血小板糖蛋白缺乏症，凸显了CD36在正常血小板功能和止血中的重要性。抗CD36抗体（SMφ）在小鼠、大鼠和人类中均表现出反应性，因此CD36（SMφ）抗体成为各种实验应用的多功能工具，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）。CD36 单克隆抗体（SMφ）支持对 CD36 在心血管疾病、代谢紊乱和免疫系统调节中的各种作用进行深入研究，从而为生理和病理机制提供宝贵的见解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD36 抗体 (SMφ) 参考文献:

  1. 膜糖蛋白 CD36：综述其在粘附, 信号转导和输血医学中的作用。  |  Greenwalt, DE., et al. 1992. Blood. 80: 1105-15. PMID: 1381234
  2. 体外巨噬细胞对凋亡伯基特淋巴瘤细胞的趋化作用：CD14 和 CD36 的作用。  |  Truman, LA., et al. 2004. Immunobiology. 209: 21-30. PMID: 15481137
  3. 脂肪细胞中 FAT/CD36 介导的长链脂肪酸吸收需要质膜筏。  |  Pohl, J., et al. 2005. Mol Biol Cell. 16: 24-31. PMID: 15496455
  4. 高级糖化终产物修饰蛋白和氧化低密度脂蛋白通过 CD36 介导小鼠脂肪细胞中瘦素的下调。  |  Unno, Y., et al. 2004. Biochem Biophys Res Commun. 325: 151-6. PMID: 15522213
  5. CD36 是二酰甘油的传感器。  |  Hoebe, K., et al. 2005. Nature. 433: 523-7. PMID: 15690042
  6. FoxO1 通过 CD36 依赖性和非依赖性机制刺激肌肉细胞中脂肪酸的摄取和氧化。  |  Bastie, CC., et al. 2005. J Biol Chem. 280: 14222-9. PMID: 15691844
  7. 在缺乏 CD36（血小板糖蛋白 IV）的血小板中，胶原蛋白可诱导正常的信号转导。  |  Daniel, JL., et al. 1994. Thromb Haemost. 71: 353-6. PMID: 7518098
  8. 单核细胞分化过程中 CD36 的合成, 加工和细胞内转运  |  Alessio, M., et al. 1996. J Biol Chem. 271: 1770-5. PMID: 8576181
  9. 鉴定 CD36 上与吞噬凋亡中性粒细胞有关的结构域（155-183）。  |  Navazo, MD., et al. 1996. J Biol Chem. 271: 15381-5. PMID: 8663130
  10. CD36 介导凝血酶原-1 对内皮细胞的体外抑制作用。  |  Dawson, DW., et al. 1997. J Cell Biol. 138: 707-17. PMID: 9245797

订购信息

产品名称产品编号规格价格数量收藏夹

CD36 抗体 (SMφ)

sc-7309
200 µg/ml
$316.00

CD36 抗体 (SMΦ) AC

sc-7309 AC
500 µg/ml, 25% agarose
$416.00

CD36 抗体 (SMΦ) Alexa Fluor® 488

sc-7309 AF488
200 µg/ml
$357.00

CD36 抗体 (SMΦ) Alexa Fluor® 594

sc-7309 AF594
200 µg/ml
$357.00

CD36 抗体 (SMΦ) Alexa Fluor® 647

sc-7309 AF647
200 µg/ml
$357.00

CD36 抗体 (SMΦ) FITC

sc-7309 FITC
200 µg/ml
$330.00

CD36 抗体 (SMΦ) HRP

sc-7309 HRP
200 µg/ml
$316.00

CD36 抗体 (SMΦ) PE

sc-7309 PE
200 µg/ml
$343.00

这个抗体是否可以用来检测心脏和心肌细胞的CD36

Asked by: BBB123
感谢您提问。 我们官网有图片展示，福尔马林固定，石蜡包埋的人心肌组织显示肌细胞的细胞质染色。 该抗体推荐用于mouse, rat 和 human 来源的CD36 WB, IP, IF, IHC(P) 和 FCM检测。 Thank you for your question. There is a picture displayed on our official website, which show the cytoplasm staining of myocardial cells in formalin-fixed and paraffin-embedded human heart muscle tissue. This antibody is recommended for detection of CD36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM.
Answered by: Ling Chen
Date published: 2024-09-02

Would it be possible to receive a sample for CD36 antibody (SMφ): sc-7309? If it works, we can commit to a purchase.

Asked by: Giordano Pula
Thank you for your question. Please contact our Technical Service Department to arrange for a sample of CD36 (SMφ): sc-7309.
Answered by: Tech Support Europe
Date published: 2021-04-08

In WB, does this Ab perform better with or without reducing agent added during denaturation (boiling)?

Asked by: ris62
Thank you for your question. The Western blot data on our website using this antibody is under reducing conditions. We have not tested the antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2020-04-03

CD36 (SMφ): sc-7309: I can not find its epitope information. May I ask whether this sc-7309 can be used as an anti-CD36 blocking antibody? Thank you.

Asked by: tjmb03
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We are not aware of any data or product citations using this antibody for blocking of CD36.
Answered by: Technical Support
Date published: 2019-08-17

Does it detect CD36 protein of monkey cells?

Asked by: Kosar
Thank you for your question. We do not have any data in monkey cells, nor are we aware of any product citations using this clone for detection of CD36 in monkey cells.
Answered by: Technical Support
Date published: 2019-05-16

does it work on 3T3-L1 adipocytes? any published paper using this cell lines ?? 

Asked by: fatmah
Thank you for your question. CD36 Antibody (SMφ): sc-7309 is recommended for the detection of CD36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM. Since the antibody is recommended for the detection of its epitope in murine samples, it should work in 3T3-L1 adipocytes without any issues. There are currently no publications featuring this antibody and 3T3-L1 adipocytes that we are aware of.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What negative control should be used when using the conjugated version of this antibody in flow cytometry?

Asked by: TinTin
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-7309 PE, then you would want to use normal mouse IgM PE, sc-2870. https://www.scbt.com/scbt/browse/antibodies-Control-IgGs-and-IgG-Conjugates/_/N-1j3ajah
Answered by: Technical Support 14
Date published: 2017-01-22
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Rated 4 out of 5 by from Anti-CD36 worked well in IHCAnti-CD36 antibody worked well in IHC using human tissues
Date published: 2025-06-06
Rated 4 out of 5 by from The antibody works wellWe used the antibody at the concentration of 1:1000 overnight, it works well.
Date published: 2022-07-14
Rated 5 out of 5 by from 功能出色Santa产品值得信懒,经过很多年沉淀下来的好品牌，为科研工作带来很多的便利条件，国内现货是其他国际品牌无法媲美的
Date published: 2022-07-14
Rated 4 out of 5 by from Buon anticorpoHo testato un campione di questo anticorpo su di un chemodectoma aortico di cane fissato in formalina ed incluso in paraffina. Ho smascherato i siti di legame con un trattamento in forno a microonde in tampone citrato 0,01 M, pH 6. L'anticorpo è stato diluito 1:50.
Date published: 2022-04-19
Rated 5 out of 5 by from SK030204V100This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2022-01-05
Rated 5 out of 5 by from Good signalGood results in immunofluorescence performed in mesenchymal stem cells
Date published: 2021-12-17
Rated 4 out of 5 by from Good antibodyThis antibody works very well on western blot analysis
Date published: 2021-05-06
Rated 5 out of 5 by from 抗体兼容性很好抗体的选购是非常谨慎的过程，选择一支满意和正确的抗体非常重要，正在使用此类抗体产品，等待获得良好实验结果！
Date published: 2021-01-10
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CD36 抗体 (SMφ) is rated 3.8 out of 5 by 30.
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