Date published: 2025-9-8
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CD32-B Antibody (F-4) - Immunohistochemistry - Image 373614
CD32-B 抗体 (F-4): sc-365864. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示内皮细胞膜和细胞质染色.
CD32-B 抗体 (F-4): sc-365864. 福尔马林固定, 石蜡包埋的人胎盘组织的免疫过氧化物酶染色显示内皮细胞膜和细胞质染色.

CD32-B 抗体 (F-4): sc-365864

4.8(4)
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说明书
  • CD32-B 抗体 F-4 是小鼠单克隆 IgG1, 在6篇文献中引用，提供 200 µg/ml
  • 特异性抗原位于human物种的CD32-B的C-terminus的氨基酸285-310之间
  • 推荐用于 human 来源的CD32-B WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取CD32-B (F-4): sc-365864的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD32-B 抗体 (F-4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

此外 CD32 抗体 包括 CD32-A, CD32-B, CD32-C和Fc γ RIIb

CD32-B抗体（F-4）是一种IgG1型小鼠单克隆CD32-B抗体，它能够通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC，P）和酶联免疫吸附测定（ELISA）等方法检测人源CD32-B蛋白。CD32-B抗体（F-4）以非共轭的抗CD32-B抗体形式提供。CD32（也被称为Fc gamma RII）是IgG聚合体的Fc片段的低亲和力受体。CD32负责巨噬细胞对免疫复合物的清除，并在B细胞调节抗体产生中起着重要作用。IgG可以非协同地结合一个或两个高度糖基化的CD32分子，这种结合向B细胞传递负信号。CD32以几种同工型的形式存在，这些同工型是由三个不同的基因A、B和C通过选择性剪接产生的。这些同工型被命名为FcgRIIA、FcgRIIB1、FcgRIIB3和FcgRIIC。所有同工型都存在于单核细胞、胎盘滋养层细胞和内皮细胞上。此外，FcgRIIB形式存在于B淋巴细胞上，而FcgRIIA和FcgRIIC形式则存在于中性粒细胞上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD32-B 抗体 (F-4) 参考文献:

  1. 昆虫细胞中产生的可溶性人 Fc γ 受体 II（CD32）异构体的表征和结晶。  |  Sondermann, P., et al. 1999. Biochemistry. 38: 8469-77. PMID: 10387093
  2. Fc 受体  |  Ravetch, JV. and Kinet, JP. 1991. Annu Rev Immunol. 9: 457-92. PMID: 1910686
  3. CD19 和 CD32b 对人类 B 细胞的反应性具有不同的调节作用。  |  Karnell, JL., et al. 2014. J Immunol. 192: 1480-90. PMID: 24442430
  4. 人 IgG Fc 受体（hFcRII；CD32）在巨噬细胞, 淋巴细胞和 IgG 转运胎盘上皮细胞中存在多种异构体。  |  Stuart, SG., et al. 1989. EMBO J. 8: 3657-66. PMID: 2531080
  5. 桥本氏甲状腺炎患者双阴性记忆 B 细胞的 CD32b 表达下调。  |  Liu, Y., et al. 2017. Mol Cell Endocrinol. 440: 1-7. PMID: 27832986
  6. 二聚体 IgG 亚类复合物与人类中性粒细胞的结合特性。  |  Huizinga, TW., et al. 1989. J Immunol. 142: 2359-64. PMID: 2784461
  7. 开发具有增强 Fc 功能的抗 CD32b 抗体，用于治疗 B 细胞和浆细胞恶性肿瘤。  |  Lu, H., et al. 2020. Mol Cancer Ther. 19: 2089-2104. PMID: 32847974
  8. DPP4/CD32b/NF-κB回路：抑制 CRP 驱动的糖尿病肾病的新型药物靶点  |  Tang, PM., et al. 2021. Mol Ther. 29: 365-375. PMID: 32956626
  9. CD32b 的均匀高表达使其成为 CAR-T 疗法治疗慢性淋巴细胞白血病的潜在靶点。  |  Wang, G., et al. 2021. J Hematol Oncol. 14: 149. PMID: 34530888
  10. CD16 和 CD32b Fc-gamma 受体调节小鼠自然杀伤细胞中抗体介导的反应。  |  Aguilar, OA., et al. 2023. J Leukoc Biol. 113: 27-40. PMID: 36822164
  11. B 淋巴细胞表面免疫球蛋白和 Fc 受体的交联可抑制通过抗原受体对肌醇磷脂分解的刺激。  |  Bijsterbosch, MK. and Klaus, GG. 1985. J Exp Med. 162: 1825-36. PMID: 3877778
  12. 免疫抑制受体 CD32b 对巨噬细胞极化的调控及其对肿瘤进展的影响  |  Chuang, HJ., et al. 2024. Int J Mol Sci. 25: PMID: 39273683

订购信息

产品名称产品编号规格价格数量收藏夹

CD32-B 抗体 (F-4)

sc-365864
200 µg/ml
RMB2377.00

CD32-B (F-4) 中和勝肽

sc-365864 P
100 µg/0.5 ml
RMB511.00

Has this antibody been tested for cross-reactivity to mouse CD32b?

Asked by: Elaine H
Thank you for your question. So far this antibody has not been found to be reactive with mouse CD32b and based on homology of the epitope we do not expect mouse cross-reactivity. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-07-13

What application is the blocking peptide sc-365864 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WBThere were three bands in human macrophage lysate, but we could recognize the specific band.
Date published: 2017-04-23
Rated 5 out of 5 by from Works as expected in WBVery strong signal in Western Blotting at a 1:200 dilution.
Date published: 2017-01-19
Rated 4 out of 5 by from Not for use on IHC-p or flow cytometryI requested a sample of this mouse monoclonal F-4 anti-CD32b to test it for unvalidated procedures. I tested whether it would work in flow cytometry by intracellular staining and for immunofluorescent imaging on formalin-fixed paraffin-embedded (FFPE) tissue. Both procedures are greatly dependent on the epitope-specificity of the antibody, and it is not surprising that this clone did not work for either application. What is encouraging is that this antibody showed almost no background (non-specific) binding in either application, suggesting a high degree of specificity. IHC-p procedure: Human spleen section. Deparaffinization, rehydration, heat-mediated epitope retrieval with either citrate pH6 or EDTA pH8, overnight staining with F-4, secondary rat anti-mouse IgG1, then tertiary goat anti-rat IgG-AlexaFluor568. Flow cytometry procedure: PBMCs. Surface stain for IgE, CCR3 and CD3. Fixation and permeabilization using BD reagents. Overnight staining with primary. Secondary rat anti-mouse IgG1-APC. Gating on CCR3+IgE+ (basophils, positive control) or CD3+ (T cells, negative control).
Date published: 2016-12-09
Rated 5 out of 5 by from Good Western Blot data of CD32 expression in nonGood Western Blot data of CD32 expression in non-transfected and human CD32 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-03-19
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CD32-B 抗体 (F-4) is rated 4.8 out of 5 by 4.
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