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CD32 Antibody (AT10) - Flow Cytometry - Image 13808
CD32 抗体 (AT10) Alexa Fluor® 488: sc-13527 AF488. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-3890.
CD32 抗体 (AT10) Alexa Fluor® 488: sc-13527 AF488. 人体外周血白细胞的 FCM 分析. 黑线直方图代表同型对照, 正常小鼠 IgG1: sc-3890.

CD32 抗体 (AT10): sc-13527

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寫評論提問

说明书
  • CD32 抗体 AT10 是小鼠单克隆 IgG1 κ，CD32抗体, 在11篇文献中引用，规格为200 µg/ml
  • 针对CD32的胞外结构域，源自human
  • CD32 抗体 (AT10) 推荐用于IP, IF 和 FCM，检测human 来源的 CD32
  • 抗CD32抗体（AT10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取CD32 (AT10): sc-13527的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD32 Antibody (AT10). 的首选辅助检测试剂，这些试剂现与CD32 Antibody (AT10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加CD32抗体，包括CD32-A, CD32-B, CD32-C和Fc γ RIIb

CD32抗体（AT10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类CD32蛋白。CD32 (AT10) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物，可满足不同实验需求。CD32，也称为Fc gamma RII，是聚集免疫球蛋白G（IgG）Fc片段的一种低亲和力受体，通过介导巨噬细胞清除免疫复合物，在免疫反应中发挥关键作用。这种功能对于维持体内平衡和防止自身免疫反应至关重要，因为IgG与CD32的结合会向B细胞传递抑制信号，从而调节抗体的产生。CD32由三个不同基因（A、B和C）的替代剪接产生多种异构体，分别命名为FcgRIIA、FcgRIIB1、FcgRIIB3和FcgRIIC。这些异构体在各种免疫细胞上表达，包括单核细胞、胎盘滋养层细胞、内皮细胞和B淋巴细胞，其中中性粒细胞上存在特定异构体，这凸显了CD32在免疫调节和反应中的多种作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD32 抗体 (AT10) 参考文献:

  1. 昆虫细胞中产生的可溶性人 Fc γ 受体 II（CD32）异构体的表征和结晶。  |  Sondermann, P., et al. 1999. Biochemistry. 38: 8469-77. PMID: 10387093
  2. 人类 NK 细胞上的功能性 CD32 分子。  |  Morel, PA., et al. 1999. Leuk Lymphoma. 35: 47-56. PMID: 10512162
  3. Fc 受体  |  Ravetch, JV. and Kinet, JP. 1991. Annu Rev Immunol. 9: 457-92. PMID: 1910686
  4. 人 IgG Fc 受体（hFcRII；CD32）在巨噬细胞, 淋巴细胞和 IgG 转运胎盘上皮细胞中存在多种异构体。  |  Stuart, SG., et al. 1989. EMBO J. 8: 3657-66. PMID: 2531080
  5. 二聚体 IgG 亚类复合物与人类中性粒细胞的结合特性。  |  Huizinga, TW., et al. 1989. J Immunol. 142: 2359-64. PMID: 2784461
  6. CD32 的表达与 T 细胞活化有关，并不是 HIV-1 病毒库的标志。  |  Badia, R., et al. 2018. Nat Commun. 9: 2739. PMID: 30013105
  7. CD32 在 HIV-1 感染过程中的作用。  |  Bertagnolli, LN., et al. 2018. Nature. 561: E17-E19. PMID: 30232425
  8. 健康与疾病中的人类 FcγRII (CD32) 白细胞 FcR 家族。  |  Anania, JC., et al. 2019. Front Immunol. 10: 464. PMID: 30941127
  9. 直接 CD32 T 细胞细胞毒性：对乳腺癌预后和治疗的影响。  |  Sconocchia, G., et al. 2022. Life Sci Alliance. 5: PMID: 36241426
  10. 免疫细胞之间通过自身抗体增强的 CD32 驱动的逆行细胞吞噬作用进行的受体转移被 HIV-1 劫持，从而感染静止的 CD4 T 细胞。  |  Albanese, M., et al. 2024. Cell Rep Med. 5: 101483. PMID: 38579727
  11. CD32 可捕获人类胚胎发育过程中的致血内皮细胞。  |  Scarfò, R., et al. 2024. Nat Cell Biol. 26: 719-730. PMID: 38594587
  12. B 淋巴细胞表面免疫球蛋白和 Fc 受体的交联可抑制通过抗原受体对肌醇磷脂分解的刺激。  |  Bijsterbosch, MK. and Klaus, GG. 1985. J Exp Med. 162: 1825-36. PMID: 3877778

订购信息

产品名称产品编号规格价格数量收藏夹

CD32 抗体 (AT10)

sc-13527
200 µg/ml
RMB2377.00

CD32 (AT10): m-IgG Fc BP-HRP 套装

sc-526553
200 µg Ab; 10 µg BP
RMB2662.00

CD32 (AT10): m-IgG1 BP-HRP 套装

sc-531926
200 µg Ab; 20 µg BP
RMB2662.00

CD32 抗体 (AT10) AC

sc-13527 AC
500 µg/ml, 25% agarose
RMB3129.00

CD32 抗体 (AT10) HRP

sc-13527 HRP
200 µg/ml
RMB2377.00

CD32 抗体 (AT10) FITC

sc-13527 FITC
200 µg/ml
RMB2482.00

CD32 抗体 (AT10) PE

sc-13527 PE
200 µg/ml
RMB2580.00

CD32 抗体 (AT10) Alexa Fluor® 488

sc-13527 AF488
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) Alexa Fluor® 546

sc-13527 AF546
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) Alexa Fluor® 594

sc-13527 AF594
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) Alexa Fluor® 647

sc-13527 AF647
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) Alexa Fluor® 680

sc-13527 AF680
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) Alexa Fluor® 790

sc-13527 AF790
200 µg/ml
RMB2685.00

CD32 抗体 (AT10) B

sc-13527 B
200 µg/ml
RMB2407.00

Dear Madam/Sir, Is it possible to request a free 10 µg sample of CD32 (AT10): sc-13527 without any conjugates? Would be very grateful as we are not sure whether the antibodies will work in our assay. Kind regards, Madina.

Asked by: Madina
Thank you for your question. Please contact our technical service directly to arrange for a free sample of this antibody in unconjugated form.
Answered by: Technical Support Europe
Date published: 2019-01-30

Can CD32 (AT10): sc-13527 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD32 (AT10): sc-13527 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Buon funzionamento un immunofluorescenzaHo testato questo anticorpo per immunofluorescenza su sezioni di tessuto in paraffina e ha funzionato molto bene, segnale chiaro e specifico; il tessuto che ho testato è polmone di criceto.
Date published: 2020-11-30
Rated 5 out of 5 by from Great FCM antibodyCD32 Antibody (AT10) produced great signal by FCM and IF
Date published: 2017-01-27
Rated 5 out of 5 by from Produced superb FCM data for THPProduced superb FCM data for THP-1 cells. -SCBT QC
Date published: 2015-06-01
Rated 5 out of 5 by from Used to immunoprecipitate CD32 from BMMNCUsed to immunoprecipitate CD32 from BMMNC (bone marrow mononuclear cells). -SCBT Publication Review
Date published: 2015-05-05
Rated 5 out of 5 by from Published western blot data using HUVECsPublished western blot data using HUVECs. -SCBT Publication Review
Date published: 2015-04-28
Rated 5 out of 5 by from Produced excellent immunofluorescence membraneProduced excellent immunofluorescence membrane staining in methanol-fixed THP-1 cells. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Worked for flow cytometry studies with humanWorked for flow cytometry studies with human aortic endothelial cells. -SCBT Publication Review
Date published: 2015-02-01
Rated 5 out of 5 by from Produced nice FCM data for human peripheralProduced nice FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-06-14
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CD32 抗体 (AT10) is rated 4.9 out of 5 by 8.
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