Date published: 2025-10-23

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CD32-A/C 抗体 (B-3): sc-373721

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说明书
  • CD32-A/C抗体(B-3)是小鼠单克隆IgG2b κ, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的CD32-A的C-terminus的氨基酸293-317之间
  • CD32-A/C 抗体 (B-3) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 CD32-A and CD32-C
  • 抗CD32-A/C抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP是CD32-A/C Antibody (B-3) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CD32-A/C Antibody (B-3) 搭配使用(请参阅下面的订购信息)。

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関連項目

CD32-A/C抗体(B-3)是一种IgG2b κ小鼠单克隆CD32-A/C抗体,可通过WB、IP、IF和ELISA检测人源的CD32-A和CD32-C。CD32-A/C抗体(B-3)既可作为非偶联的抗CD32-A/C抗体形式,也可作为多种偶联形式的抗CD32-A/C抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD32(也称为Fc gamma RII)是聚集IgG的Fc片段的低亲和力受体。CD32负责巨噬细胞清除免疫复合物,并在调节B细胞产生抗体方面发挥重要作用。IgG可以非协同地结合一个或两个高度糖基化的CD32分子,这种结合为B细胞传递负信号。CD32以几种异构体形式存在,这些异构体由三个不同基因A、B和C的选择性剪接产生。这些异构体被称为FcgRIIA、FcgRIIB1、FcgRIIB3和FcgRIIC。所有异构体均存在于单核细胞、胎盘滋养层和内皮细胞上。此外,FcgRIIB形式存在于B淋巴细胞上,而FcgRIIA和FcgRIIC形式则存在于中性粒细胞上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD32-A/C 抗体 (B-3) 参考文献:

  1. 昆虫细胞中产生的可溶性人 Fc γ 受体 II(CD32)异构体的表征和结晶。  |  Sondermann, P., et al. 1999. Biochemistry. 38: 8469-77. PMID: 10387093
  2. 血液单核细胞和细胞系中 CD32A(Fc γ RIIa)mRNA 的表达和调节。  |  Alevy, YG., et al. 1992. Mol Immunol. 29: 1289-97. PMID: 1406718
  3. 人中性粒细胞中交联的 opsonic 受体 CD32A(FcgammaRIIA)对高密度去污剂抗性膜域的募集。  |  Rollet-Labelle, E., et al. 2004. Biochem J. 381: 919-28. PMID: 15130090
  4. Fc 受体  |  Ravetch, JV. and Kinet, JP. 1991. Annu Rev Immunol. 9: 457-92. PMID: 1910686
  5. 人 IgG Fc 受体(hFcRII;CD32)在巨噬细胞, 淋巴细胞和 IgG 转运胎盘上皮细胞中存在多种异构体。  |  Stuart, SG., et al. 1989. EMBO J. 8: 3657-66. PMID: 2531080
  6. CD32a 抗体会诱发 FCGR2A 小鼠血小板减少和 II 型超敏反应。  |  Meyer, T., et al. 2015. Blood. 126: 2230-8. PMID: 26396093
  7. 二聚体 IgG 亚类复合物与人类中性粒细胞的结合特性。  |  Huizinga, TW., et al. 1989. J Immunol. 142: 2359-64. PMID: 2784461
  8. 质控细胞分析揭示了 CD32a+ CD4+ T 细胞从早期 HIV 感染到有效 cART 的分布和动态变化。  |  Coindre, S., et al. 2018. Front Immunol. 9: 1217. PMID: 29915583
  9. CD32A嵌合受体T细胞与西妥昔单抗或帕尼单抗联用,体外清除表皮生长因子受体过表达的癌细胞。  |  Caratelli, S., et al. 2020. Int J Cancer. 146: 236-247. PMID: 31479522
  10. FcRn 是一种 CD32a 核心受体,它决定了对 IgG 免疫复合物驱动的自身免疫的易感性。  |  Hubbard, JJ., et al. 2020. J Exp Med. 217: PMID: 32658257
  11. B 淋巴细胞表面免疫球蛋白和 Fc 受体的交联可抑制通过抗原受体对肌醇磷脂分解的刺激。  |  Bijsterbosch, MK. and Klaus, GG. 1985. J Exp Med. 162: 1825-36. PMID: 3877778
  12. CD32C(Fc γ RIIC)mRNA 的表达和调节。  |  Alevy, YG., et al. 1993. Mol Immunol. 30: 775-82. PMID: 8502244

订购信息

产品名称产品编号规格价格数量收藏夹

CD32-A/C 抗体 (B-3)

sc-373721
200 µg/ml
RMB2377.00

CD32-A/C (B-3): m-IgG Fc BP-HRP 套装

sc-525919
200 µg Ab; 10 µg BP
RMB2662.00

CD32-A/C 抗体 (B-3) AC

sc-373721 AC
500 µg/ml, 25% agarose
RMB3129.00

CD32-A/C 抗体 (B-3) HRP

sc-373721 HRP
200 µg/ml
RMB2377.00

CD32-A/C 抗体 (B-3) FITC

sc-373721 FITC
200 µg/ml
RMB2482.00

CD32-A/C 抗体 (B-3) PE

sc-373721 PE
200 µg/ml
RMB2580.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 488

sc-373721 AF488
200 µg/ml
RMB2685.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 546

sc-373721 AF546
200 µg/ml
RMB2685.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 594

sc-373721 AF594
200 µg/ml
RMB2685.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 647

sc-373721 AF647
200 µg/ml
RMB2685.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 680

sc-373721 AF680
200 µg/ml
RMB2685.00

CD32-A/C 抗体 (B-3) Alexa Fluor® 790

sc-373721 AF790
200 µg/ml
RMB2685.00

CD32-A/C (B-3) 中和勝肽

sc-373721 P
100 µg/0.5 ml
RMB511.00

For the CD32A/C antibody, does one band correspond to A and one to C? Would it be possible to get a sample to test this antibody? I am wanting an antibody to just CD32A, but I see you discontinued it... Thanks, Lisa

Asked by: Lisa K
Thank you for your question. We have not characterized whether or not the two bands observed in the Western blot data on our website represent CD32A and C separately. CD32A has a predicted molecular weight of 35 kDa, while CD32C has a similar, but lower predicted molecular weight of 26-30 kDa. Yes, right now we are offering a free 10 µg sample of this monoclonal antibody, CD32-A/C (B-3): sc-373721. Please contact our Technical Service Department to request a free sample to test before you place your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-20

What application is the blocking peptide sc-373721 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody, Great for WBGreat for detecting CD32 of human origin via WB, very strong and clear signal with little background.
Date published: 2017-02-09
Rated 5 out of 5 by from Great Western Blot data of CD32Great Western Blot data of CD32-A expression in non-transfected 293T, human CD32-A transfected 293T and U-937 whole cell lysates. -SCBT QC
Date published: 2015-05-02
Rated 5 out of 5 by from Good Western Blot data of CD32Good Western Blot data of CD32-A/C expression in U-937 and AML-193 whole cell lysates. -SCBT QC
Date published: 2013-06-26
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CD32-A/C抗体 (B-3) is rated 5.0 out of 5 by 3.
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