Date published: 2025-9-9
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CD30 Antibody (Ber-H2) - Western Blotting - Image 379331
CD30 抗体 (Ber-H2): sc-19658. Western 印迹分析 Jurkat 全细胞裂解液中 CD30 的表达.
CD30 抗体 (Ber-H2): sc-19658. Western 印迹分析 Jurkat 全细胞裂解液中 CD30 的表达.

CD30 抗体 (Ber-H2): sc-19658

4.3(8)
寫評論提問

说明书
  • CD30抗体(Ber-H2)是小鼠单克隆IgG1 κ, 在7篇文献中引用，规格为200 µg/ml
  • 与Co细胞细胞悬液结合
  • CD30 抗体 (Ber-H2) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 CD30
  • 抗CD30抗体（Ber-H2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD30 (Ber-H2): sc-19658的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD30 Antibody (Ber-H2) for WB applications. 的首选辅助检测试剂，这些试剂现与CD30 Antibody (Ber-H2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD30抗体（Ber-H2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类来源的CD30蛋白。CD30（Ber-H2）抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD30是肿瘤坏死因子受体家族的一员，在调节免疫反应中起着至关重要的作用，主要表达于活化的T和B淋巴细胞以及某些肿瘤细胞中，尤其是霍奇金淋巴瘤和其他血液系统恶性肿瘤。CD30的结构以富含胱氨酸的胞外结构域为特征，对于与配体（如CD30L）的相互作用至关重要，根据细胞环境，配体可以促进细胞增殖或发挥抗增殖作用。这种双重功能凸显了CD30在正常免疫调节和恶性肿瘤发病机理中的重要性，使抗CD30抗体（Ber-H2）成为研究免疫反应和癌症生物学的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD30 抗体 (Ber-H2) 参考文献:

  1. 使用 Brentuximab Vedotin 靶向 CD30 治疗霍奇金淋巴瘤  |  Alperovich, A. and Younes, A. 2016. Cancer J. 22: 23-6. PMID: 26841013
  2. 霍奇金淋巴瘤和其他 B 细胞淋巴瘤中的 CD30 和 CD30 靶细胞疗法。  |  Bhatt, G., et al. 2016. Curr Hematol Malig Rep. 11: 480-491. PMID: 27613003
  3. 了解 CD30 生物学和靶向治疗：从历史角度洞察未来方向。  |  van der Weyden, CA., et al. 2017. Blood Cancer J. 7: e603. PMID: 28885612
  4. 原发性皮肤 CD30+ 淋巴细胞增生性疾病综述  |  Chen, C., et al. 2019. Hematol Oncol Clin North Am. 33: 121-134. PMID: 30497669
  5. ADAM10作为CD30脱落酶在典型霍奇金淋巴瘤中的作用  |  Hansen, HP., et al. 2020. Front Immunol. 11: 398. PMID: 32296414
  6. CD30 配体信号转导涉及霍奇金淋巴瘤细胞系中酪氨酸激酶和丝裂原活化蛋白激酶的激活。  |  Wendtner, CM., et al. 1995. Cancer Res. 55: 4157-61. PMID: 7545087
  7. 肿瘤坏死因子配体超家族：参与恶性淋巴瘤的病理学研究。  |  Gruss, HJ. and Dower, SK. 1995. Blood. 85: 3378-404. PMID: 7780126
  8. 肿瘤坏死因子受体超家族成员及其配体。  |  Armitage, RJ. 1994. Curr Opin Immunol. 6: 407-13. PMID: 7917108
  9. CD27：T 细胞活化的标记物和介质？  |  Hintzen, RQ., et al. 1994. Immunol Today. 15: 307-11. PMID: 8086099
  10. CD30 抗原是霍奇金淋巴瘤的标志物，它是一种受体，其配体定义了与 TNF 同源的新兴细胞因子家族。  |  Smith, CA., et al. 1993. Cell. 73: 1349-60. PMID: 8391931
  11. 小鼠 CD30 的结构和表达及其在细胞因子产生中的作用。  |  Bowen, MA., et al. 1996. J Immunol. 156: 442-9. PMID: 8543792
  12. CD27-CD70 相互作用：揭示其在正常和肿瘤性 B 细胞生长中的作用。  |  Lens, SM., et al. 1995. Leuk Lymphoma. 18: 51-9. PMID: 8580829

订购信息

产品名称产品编号规格价格数量收藏夹

CD30 抗体 (Ber-H2)

sc-19658
200 µg/ml
RMB2377.00

CD30 (Ber-H2): m-IgG Fc BP-HRP 套装

sc-526587
200 µg Ab; 10 µg BP
RMB2662.00

CD30 (Ber-H2): m-IgG1 BP-HRP 套装

sc-531960
200 µg Ab; 20 µg BP
RMB2662.00

CD30 抗体 (Ber-H2) AC

sc-19658 AC
500 µg/ml, 25% agarose
RMB3129.00

CD30 抗体 (Ber-H2) HRP

sc-19658 HRP
200 µg/ml
RMB2377.00

CD30 抗体 (Ber-H2) FITC

sc-19658 FITC
200 µg/ml
RMB2482.00

CD30 抗体 (Ber-H2) PE

sc-19658 PE
200 µg/ml
RMB2580.00

CD30 抗体 (Ber-H2) Alexa Fluor® 488

sc-19658 AF488
200 µg/ml
RMB2685.00

CD30 抗体 (Ber-H2) Alexa Fluor® 546

sc-19658 AF546
200 µg/ml
RMB2685.00

CD30 抗体 (Ber-H2) Alexa Fluor® 594

sc-19658 AF594
200 µg/ml
RMB2685.00

CD30 抗体 (Ber-H2) Alexa Fluor® 647

sc-19658 AF647
200 µg/ml
RMB2685.00

CD30 抗体 (Ber-H2) Alexa Fluor® 680

sc-19658 AF680
200 µg/ml
RMB2685.00

CD30 抗体 (Ber-H2) Alexa Fluor® 790

sc-19658 AF790
200 µg/ml
RMB2685.00

What is the recommended starting dilution for Western blot for CD30 (Ber-H2): sc-19658?

Asked by: Trav11
Thank you for your question. This particular CD30 antibody (sc-19658) is only recommended for Flow Cytometry and Immunofluorescence applications. Consider using the CD30 Antibody (C-3): sc-46683, which is recommended for use in WB, IP, IF and ELISA applications. A complete list of available CD30 antibodies can be found at the link below: https://www.scbt.com/scbt/browse/antibodies-Primary-Antibodies-lymphocyte-signaling-antibodies-cd30-Antibodies/_/N-1knao44 If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 8
Date published: 2017-01-16

Does CD30 (Ber-H2): sc-19658 work for IF in Porcine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 1
Date published: 2017-01-13
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Rated 4 out of 5 by from Good results on feline FFPE lymph nodeTested with a 1:100 protocol (Tris-EDTA pH 9.0) on a feline lymph node and the results were good.
Date published: 2023-01-19
Rated 5 out of 5 by from Strong FCM dataGreat product for FCM studies;highly specific signal
Date published: 2017-01-14
Rated 5 out of 5 by from Excellent!This antibody works very well in Flow Cytometry. Great choice!
Date published: 2016-12-22
Rated 4 out of 5 by from Suitable for IHCSuitable for IHC. Detects a cytoplasmic/membraneous signal in embryonal carcinomas (EC) and EC-like cells. Used at 1:200.<br /> <br /> For further information see:<br /> http://www.ncbi.nlm.nih.gov/pubmed/26226633
Date published: 2015-10-08
Rated 4 out of 5 by from FACS publishable data with human blood eosinophilFACS publishable data with human blood eosinophil apoptosis - SCBT Publication Review
Date published: 2015-07-22
Rated 4 out of 5 by from Robust FCM data of MOLTRobust FCM data of MOLT-4 cells. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Publishable IHC data with human lymph NodePublishable IHC data with human lymph Node - SCBT Publication Review
Date published: 2015-05-15
Rated 4 out of 5 by from Good FCM data of PMAGood FCM data of PMA-stimulated Jurkat cells. -SCBT QC
Date published: 2013-12-19
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CD30 抗体 (Ber-H2) is rated 4.3 out of 5 by 8.
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