Date published: 2026-10-19

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CD3-ζ Antibody (E-3) - Western Blotting - Image 370882
CD3-ζ 抗体 (E-3): sc-166435. Jurkat (A), SUP-T1 (B), CCRF-CEM (C) 和 MOLT-4 (D) 全细胞裂解液中 CD3-ζ 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
CD3-ζ 抗体 (E-3): sc-166435. Jurkat (A), SUP-T1 (B), CCRF-CEM (C) 和 MOLT-4 (D) 全细胞裂解液中 CD3-ζ 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

CD3-ζ 抗体 (E-3): sc-166435

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说明书
  • CD3-ζ抗体(E-3)是小鼠单克隆IgG3 κ, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CD3-ζ的C-terminus的氨基酸137-163之间
  • 推荐用于 mouse, rat 和 human 来源的CD3-ζ WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 看 CD3-ζ (6B10.2): sc-1239，可查看有标记的 CD3-ζ 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgGκ BP-HRP是CD3-ζ Antibody (E-3) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与CD3-ζ Antibody (E-3) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。
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描述
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研究信息

関連項目

附加CD3抗体，包括CD3-δ, CD3-ε, CD3-γ和CD3-ζ

CD3-ζ抗体（E-3）是一种IgG3 κ小鼠单克隆CD3-ζ抗体（也称为CD3-ζ抗体或CD247抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CD3-ζ。CD3-ζ抗体（E-3）可作为非偶联抗CD3-ζ抗体形式提供。T细胞抗原受体（TCR）识别外来抗原，并将这种识别事件转化为细胞内信号，从而引发细胞从休眠状态到激活状态的变化。这个信号传导过程的大部分可归因于与TCR直接相关的多亚基蛋白质复合物。这个复合物被命名为CD3（分化簇3）。它由五个不变的多肽链组成，这些多肽链结合形成三个二聚体：一个由γ和ε链组成的异二聚体（γε），一个由δ和ε链组成的异二聚体（δε）和两个ζ链组成的同二聚体（ζζ）或ζ和η链组成的异二聚体（ζη）。ζ和η链由同一基因编码，但由于选择性剪接事件，它们的羧基末端不同。γ、ε和δ链各包含一个保守的免疫受体酪氨酸基激活基序（ITAM）的单拷贝。相比之下，ζ链包含三个连续的相同基序拷贝。磷酸化的ITAMs作为ZAP-70和Syk等蛋白激酶的停靠位点，也能够调节它们的激酶活性。已解析出ZAP-70 SH2结构域与ζ链ITAMs结合的晶体结构。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD3-ζ 抗体 (E-3) 参考文献:

  1. L-精氨酸调节活化的人T淋巴细胞中的CD3zeta表达和T细胞功能。  |  Zea, AH., et al. 2004. Cell Immunol. 232: 21-31. PMID: 15922712
  2. TNF-α对出生后大鼠海马中CD3-zeta和MHC-I的影响。  |  Sourial-Bassillious, N., et al. 2006. Pediatr Res. 60: 377-81. PMID: 16940250
  3. TCR-CD3zeta 信号转导缺陷介导肠系膜淋巴结中的 T 细胞低反应性。  |  Yi, HJ., et al. 2008. Mol Immunol. 45: 3748-55. PMID: 18619676
  4. T细胞受体活化的成像显示，酪氨酸磷酸化CD3ζ在胞内区积聚。  |  Yudushkin, IA. and Vale, RD. 2010. Proc Natl Acad Sci U S A. 107: 22128-33. PMID: 21135224
  5. 细胞外 2'5'-oligoadenylate synthetase 2 在口腔癌中通过 caspase-3 激活介导 T 细胞受体 CD3-ζ 链下调。  |  Dar, AA., et al. 2016. Immunology. 147: 251-64. PMID: 26595239
  6. TCR交联促进Crk适配蛋白与酪氨酸磷酸化CD3ζ链的结合。  |  Dong, G., et al. 2017. Biochem Biophys Res Commun. 488: 541-546. PMID: 28526413
  7. T细胞CD3-zeta链的合成失败：部分T细胞受体复合物的结构和功能。  |  Sussman, JJ., et al. 1988. Cell. 52: 85-95. PMID: 3278811
  8. CD3ζ适配器结构决定了人类和鼠类CD16 Fc受体信号传导之间的功能差异。  |  Aguilar, OA., et al. 2022. J Exp Med. 219: PMID: 35320345
  9. 基于生物素结合的单链链霉抗生物素蛋白的第三代CAR（CD28-4-1BB-CD3-ζ）工程化T细胞具有高度特异性，可用于潜在的肿瘤免疫治疗。  |  Gallego-Valle, J., et al. 2024. Front Immunol. 15: 1448752. PMID: 39364400
  10. 小鼠CD3 zeta/eta/phi/Oct-1基因座内的靶向破坏。  |  Koyasu, S., et al. 1994. EMBO J. 13: 784-97. PMID: 8112294

订购信息

产品名称产品编号规格价格数量收藏夹

CD3-ζ 抗体 (E-3)

sc-166435
200 µg/ml
$322.00

CD3-ζ (E-3): m-IgGκ BP-HRP 套装

sc-521608
200 µg Ab, 40 µg BP
$361.00

CD3-ζ (E-3) 中和勝肽

sc-166435 P
100 µg/0.5 ml
$69.00

Can you recommend a negative control to use with CD3-ζ (E-3): sc-166435 monoclonal antibody?

Asked by: jenni
We recommend using the blocking peptide sc-166435 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 15
Date published: 2017-01-12

Is CD3-ζ (E-3): sc-166435 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using CD3-ζ (6B10.2): sc-1239, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/cd3-zeta-antibody-6b10-2)
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 2 out of 5 by cesarfdm from Not ideal for immuno-staining HEK cellsI tried staining HEK293 cells that were transiently expressing a construct with the CD3 peptide and found that the signal was not very good compared to a parallel immuno-staining with another primary antibody
Date published: 2019-06-05
Rated 5 out of 5 by Susiea from good effectOur aim is to use T cell antigen receptor recognition of foreign antigens, and this recognition events into intracellular signals, causing cells from dormant to active state changes. This is a very good effect
Date published: 2017-02-09
Rated 5 out of 5 by Sweetums from Great for Western blotGood CD-3 zeta antibody; gives great bands in Western blot. Cross-reactive with multiple species--mouse, rat, human, canine, bovine, porcine. Blocking peptide good as a negative control to check specificity.
Date published: 2017-01-27
Rated 5 out of 5 by LR from Good monoclonal antibodyGood monoclonal antibody, favorable data produced in WB on Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by LM from Superb antibody for Western BlotSuperb antibody for Western Blot. Good specificity against human CD3- transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-07-04
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CD3-ζ 抗体 (E-3) is rated 4.4 out of 5 by 5.
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