Date published: 2025-9-6
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CD3-ζ Antibody (6B10.2) - Western Blotting - Image 377844
CD3-ζ 抗体 (6B10. 2): sc-1239. Jurkat (A), CCRF-CEM (B) 和 SUP-T1 (C) 全细胞裂解液中 CD3-ζ 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 使用的检测试剂: m-IgGκ BP-CFL 790: sc-516181.
CD3-ζ 抗体 (6B10. 2): sc-1239. Jurkat (A), CCRF-CEM (B) 和 SUP-T1 (C) 全细胞裂解液中 CD3-ζ 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 使用的检测试剂: m-IgGκ BP-CFL 790: sc-516181.

CD3-ζ 抗体 (6B10.2): sc-1239

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说明书
  • CD3-zeta 抗体 6B10.2 是小鼠单克隆 IgG1 κ，CD3-ζ抗体, 在275篇文献中引用，规格为200 µg/ml
  • 针对氨基酸89-105，在human起源的CD3-ζ的细胞质域内进行映射
  • CD3-zeta 抗体 (6B10.2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 CD3-ζ
  • 抗CD3-zeta抗体（6B10.2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联PerCP 以及 Alexa Fluor® 405 用于IF, IHC(P) and FCM
  • 关于如何获取CD3-ζ (6B10.2): sc-1239的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 CD3-ζ 抗体 (6B10.2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD3-ζ 抗体 (6B10.2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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说明书与实验方案
研究信息

関連項目

此外 CD3 抗体 包括 CD3-δ, CD3-ε, CD3-γ和CD3-ζ

CD3-ζ抗体（6B10.2）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类的CD3-zeta。CD3-ζ (6B10.2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。CD3-zeta在T细胞活化和信号传导中起着至关重要的作用，因为CD3-zeta是T细胞受体（TCR）复合物的重要组成部分，而TCR对于免疫反应至关重要。CD3-zeta位于T细胞的质膜上，是一个多亚基复合物的一部分，在TCR识别外来抗原时传递信号。这种信号级联对于激活T细胞至关重要，可导致其增殖并分化成效应细胞，从而瞄准并消灭病原体。CD3-zeta的结构包含三个免疫受体酪氨酸基激活基序（ITAM），可有效招募信号分子（如ZAP-70），进一步传播激活信号。CD3-zeta能够与其他CD3成分形成同源二聚体或异源二聚体，从而增强T细胞信号的复杂性和特异性，使CD3-zeta成为免疫学和治疗干预研究的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD3-ζ 抗体 (6B10.2) 参考文献:

  1. T 细胞抗原受体的结构, 组装和细胞内转运。  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
  2. T 细胞抗原受体的信号转导。  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
  3. CD3ζ ITAMs通过抑制TCR信号传导来识别低亲和力肽并发挥拮抗作用。  |  Gaud, G., et al. 2023. Nat Immunol. 24: 2121-2134. PMID: 37945821
  4. ζ缺陷CAR整合到CD3ζ基因中，在T和NK细胞中传递强大的细胞毒性。  |  Kath, J., et al. 2024. Blood. 143: 2599-2611. PMID: 38493479
  5. CD28共刺激通过LCK/CD3ζ/ZAP70信号传导轴增强NK细胞中的CAR信号传导。  |  Acharya, S., et al. 2024. Cancer Discov. 14: 1879-1900. PMID: 38900051
  6. CD3-zeta突变小鼠中的T细胞发育。  |  Huang, MT. 1995. Int Rev Immunol. 13: 29-41. PMID: 7494107
  7. 信号转导。Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
  8. 蛋白酪氨酸激酶 ZAP-70 与 T 细胞受体相互作用的分子基础。  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
  9. ZAP-70 与磷酸化的 T 细胞受体 zeta 和 eta 结合可增强其自身磷酸化，并为含 SH2 结构域的蛋白质生成特异性结合位点。  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
  10. CD3 zeta 家族二聚体的不同细胞质结构可调节 T 细胞的激活信号和功能。  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
  11. 蛋白酪氨酸激酶和蛋白酪氨酸磷酸酶在 T 细胞抗原受体信号转导中的作用。  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
  12. 靶向干扰 CD3 eta 基因座导致小鼠高致死率：Oct-1 转录在反向链上的调节。  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747

订购信息

产品名称产品编号规格价格数量收藏夹

CD3-ζ 抗体 (6B10.2)

sc-1239
200 µg/ml
RMB2377.00

CD3-ζ (6B10.2): m-IgG Fc BP-HRP 套装

sc-528127
200 µg Ab; 10 µg BP
RMB2662.00

CD3-ζ (6B10.2): m-IgGκ BP-HRP 套装

sc-520430
200 µg Ab, 40 µg BP
RMB2662.00

CD3-ζ (6B10.2): m-IgG1 BP-HRP 套装

sc-542778
200 µg Ab; 20 µg BP
RMB2662.00

CD3-ζ 抗体 (6B10.2) AC

sc-1239 AC
500 µg/ml, 25% agarose
RMB3129.00

CD3-ζ 抗体 (6B10.2) HRP

sc-1239 HRP
200 µg/ml
RMB2377.00

CD3-ζ 抗体 (6B10.2) FITC

sc-1239 FITC
200 µg/ml
RMB2482.00

CD3-ζ 抗体 (6B10.2) PE

sc-1239 PE
200 µg/ml
RMB2580.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 488

sc-1239 AF488
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 546

sc-1239 AF546
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 594

sc-1239 AF594
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 647

sc-1239 AF647
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 680

sc-1239 AF680
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 790

sc-1239 AF790
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) Alexa Fluor® 405

sc-1239 AF405
200 µg/ml
RMB2685.00

CD3-ζ 抗体 (6B10.2) PerCP

sc-1239 PerCP
100 tests in 2 ml
RMB2640.00

请问这个抗体4度可以储存多久呀

Asked by: 请叫我CC
感谢您的提问。我们产品自订单之日起质保一年，所以至少可以储存一年。 Thank you for your question. Our products are guaranteed for one year from the date of order, so they can be stored for at least one year.
Answered by: Zhanglong
Date published: 2024-12-30

In the description "raised against amino acids 36-54 mapping within an N-terminal domain of CD3-ζ of human origin" However, aa 36-54 is almost in the TM domain (31-51). How do you define aa 36-54? with or without leader? can it detect cytoplasmic one

Asked by: Leo_
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We do have IHC(P) data on our website using this antibody to detect membrane and cytoplasmic staining in formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2020-10-23

Is the agarose-conjugated CD3-ζ Antibody (6B10.2): sc-1239 provided as 500µg/mL, unlike the other formats that come as 200µg/mL? The recommended concentration for IP is 1-2µg/mL--does this mean that you would only need 2-4µL agarose-conj. Ab per 1mL IP?

Asked by: eleonard
Thank you for your question. For the agarose conjugated antibody, sc-1239 AC, we recommend using ~ 20uL of a 25% slurry, which equals 10 ug of ab/5 uL of agarose beads. Each vial contains 500 ug of antibody/250 uL of agarose in 1 mL of slurry (PBS + Sodium Azide + conjugated antibodies).
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended dilution for IHC/P application?

Asked by: UB948
Thank you for your question. The recommended starting dilution for IHC(P) as described in the antibody’s datasheet is 1:50 with a dilution range of 1:50-1:500.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CD3-ζ Antibody (6B10.2): sc-1239?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-10

I am going to be performing IHC in mouse tissues. How can I expect this antibody to work?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-1239, is available conjugated to FITC, AF647, and AF488.
Answered by: TechService7
Date published: 2017-01-04
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Rated 5 out of 5 by from greatThe antibody works great, with very clear blotting. Highly recommend
Date published: 2024-04-11
Rated 5 out of 5 by from 特異的な反応が見られたウエスタンブロットにおいて他社製品の抗CD3ζ抗体で検出出来なかったタンパク質が、この製品では検出することが出来ました。
Date published: 2020-05-29
Rated 5 out of 5 by from anti-CD3z AntibodyThe antibody usualy comes in very quickly; however, this order took a while because they were on backorder. Still, no problem. The antibody works great, with very clear blotting. Highly recommend!
Date published: 2018-08-29
Rated 5 out of 5 by from 16 KDa bandThis antibody is very good quality , 1:1000, still strong signal. I got only one specific band at 16 KDa
Date published: 2017-11-21
Rated 5 out of 5 by from Great product!I got this product two months ago. It did a great job when i used it to detect the CD3+ T cells by FCM. The high purity and specificity makes this antibody works well!
Date published: 2017-09-10
Rated 5 out of 5 by from we used it to detect the cd3ζ of CAR T cellsI bought this a year ago and used it to detect the cd3ζ of CAR T cells by WB.As it turned out, this product is enable for this experiment.
Date published: 2017-09-04
Rated 5 out of 5 by from Specific bandWe used at 1:2000 for detection in Jurkat cells, and got only one specific band at 15kd
Date published: 2017-08-18
Rated 5 out of 5 by from good result used in WBI always had good result s with this CD3-ζ detection use this antibody.1:600 diluted the antibody.and use the goat anti-mouse secondary from Jackson.I suggest use ECL system from Santa cruz,it suiable for weak expression detection.
Date published: 2017-07-22
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CD3-ζ 抗体 (6B10.2) is rated 5.0 out of 5 by 22.
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