Date published: 2025-9-8
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CD28 Antibody (CD28.2) - Western Blotting - Image 17934
CD28 抗体 (CD28. 2): sc-19655. Western 印迹分析 Jurkat 全细胞裂解液中 CD28 的表达.
CD28 抗体 (CD28. 2): sc-19655. Western 印迹分析 Jurkat 全细胞裂解液中 CD28 的表达.

CD28 抗体 (CD28.2): sc-19655

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说明书
  • CD28抗体(CD28.2)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 针对 human CD28 转染鼠 T 细胞杂交瘤
  • CD28 抗体 (CD28.2) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 CD28
  • 抗CD28抗体（CD28.2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD28 (CD28.2): sc-19655的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD28 Antibody (CD28.2) for WB applications. 的首选辅助检测试剂，这些试剂现与CD28 Antibody (CD28.2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD28抗体（CD28.2）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类CD28蛋白。抗CD28抗体（CD28.2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD28蛋白在T细胞活化和存活中起着至关重要的作用，它是一种主要的共刺激受体，通过与抗原呈递细胞上的CD80和CD86配体结合，增强T细胞的反应。这种相互作用对于T细胞有效增殖和淋巴因子产生至关重要，而淋巴因子对于协调免疫反应必不可少。CD28主要在静止的T细胞上表达，而CTLA-4在活化的T细胞上充当共抑制受体，与相同的配体结合，具有更高的亲和力。CD28和CTLA-4信号之间的平衡对于维持免疫平衡和防止自身免疫至关重要。此外，其他受体（如SLAM）的存在可以进一步调节T细胞的扩张，不受CD28的影响，这凸显了T细胞调节的复杂性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD28 抗体 (CD28.2) 参考文献:

  1. 一种新型 CD28 mRNA 变体和多种 CD28 mRNA 异构体同时存在于人类 T 淋巴细胞中。  |  Deshpande, M., et al. 2002. Hum Immunol. 63: 20-3. PMID: 11916166
  2. 非正常 CD4+ CD28- T 细胞在急性冠状动脉综合征中的作用。  |  Sun, W., et al. 2012. Mol Biol Rep. 39: 3337-42. PMID: 21695428
  3. 支柱》文章：CD28和CTLA-4对T细胞对刺激的反应具有相反的作用。实验医学杂志》。1995.182:459-465.  |  Krummel, MF. and Allison, JP. 2011. J Immunol. 187: 3459-65. PMID: 21934097
  4. CTLA-4/CD28 轴对类风湿关节炎关节炎症过程的影响。  |  Körmendy, D., et al. 2013. Arthritis Rheum. 65: 81-7. PMID: 23045162
  5. CD28-CD80/86 及其负调控因子 CTLA-4 对 T 细胞的共同刺激对动脉粥样硬化的加速发展有很大影响。  |  Ewing, MM., et al. 2013. Int J Cardiol. 168: 1965-74. PMID: 23351788
  6. T 细胞共同刺激和共同抑制的分子机制。  |  Chen, L. and Flies, DB. 2013. Nat Rev Immunol. 13: 227-42. PMID: 23470321
  7. 通过控制 CTLA-4 和 IL-2 的表达，从传统 T 细胞中构建自我识别调节性 T 细胞。  |  Yamaguchi, T., et al. 2013. Proc Natl Acad Sci U S A. 110: E2116-25. PMID: 23690575
  8. 人工抗原递呈细胞加 IL-15 和 IL-21 能有效诱导黑色素瘤特异性细胞毒性 CD8+ CD28+ T 淋巴细胞反应。  |  Yu, X., et al. 2013. Asian Pac J Trop Med. 6: 467-72. PMID: 23711708
  9. CD8+CD28- 免疫增强 T 细胞在癌症免疫学中不断演变的作用。  |  Huff, WX., et al. 2019. Int J Mol Sci. 20: PMID: 31181772
  10. 衰老的 CD4+CD28- T 淋巴细胞是牙周炎期间 Th17/Treg 失衡和牙槽骨吸收的潜在驱动因素。  |  González-Osuna, L., et al. 2022. Int J Mol Sci. 23: PMID: 35269683
  11. CD28/B7 对自身免疫性糖尿病的调控。  |  Herold, KG., et al. 1997. Immunol Res. 16: 71-84. PMID: 9048209
  12. CTLA-4缺陷小鼠的淋巴细胞增殖是由CD4+ T细胞的成本刺激依赖性活化介导的。  |  Chambers, CA., et al. 1997. Immunity. 7: 885-95. PMID: 9430233

订购信息

产品名称产品编号规格价格数量收藏夹

CD28 抗体 (CD28.2)

sc-19655
200 µg/ml
RMB2377.00

CD28 (CD28.2): m-IgG Fc BP-HRP 套装

sc-526586
200 µg Ab; 10 µg BP
RMB2662.00

CD28 (CD28.2): m-IgG1 BP-HRP 套装

sc-531959
200 µg Ab; 20 µg BP
RMB2662.00

CD28 抗体 (CD28.2) AC

sc-19655 AC
500 µg/ml, 25% agarose
RMB3129.00

CD28 抗体 (CD28.2) HRP

sc-19655 HRP
200 µg/ml
RMB2377.00

CD28 抗体 (CD28.2) FITC

sc-19655 FITC
200 µg/ml
RMB2482.00

CD28 抗体 (CD28.2) PE

sc-19655 PE
200 µg/ml
RMB2580.00

CD28 抗体 (CD28.2) Alexa Fluor® 488

sc-19655 AF488
200 µg/ml
RMB2685.00

CD28 抗体 (CD28.2) Alexa Fluor® 546

sc-19655 AF546
200 µg/ml
RMB2685.00

CD28 抗体 (CD28.2) Alexa Fluor® 594

sc-19655 AF594
200 µg/ml
RMB2685.00

CD28 抗体 (CD28.2) Alexa Fluor® 647

sc-19655 AF647
200 µg/ml
RMB2685.00

CD28 抗体 (CD28.2) Alexa Fluor® 680

sc-19655 AF680
200 µg/ml
RMB2685.00

CD28 抗体 (CD28.2) Alexa Fluor® 790

sc-19655 AF790
200 µg/ml
RMB2685.00

What is the amino acids sequence of CD28.2(anti-CD28 antibody) ?

Asked by: xinxin
Thank you for your questions. We do not characterize the exact binding site of the antibody . The antibody was raised against the full length of human origin.
Answered by: BlakeJ
Date published: 2022-09-02

Dear Colleagues. Do you have available or can you prepare biotinylated anti-human CD28 antibodies? If so, what is the IgG class of the monoclonal antibodies available

Asked by: SHIMON SLAVIN
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What is the sequence of the target epitope for this antibody? Is it in the extracellular region of CD28?

Asked by: davidelias
Thank you for your question. This mouse monoclonal antibody, CD28 (CD28.2): sc-19655, is raised against human CD28 transfected murine T cell hybridoma. The exact epitope recognized by the antibody has not been determined. We do not know whether the epitope is extracellular or intracellular for this clone.
Answered by: Technical Support
Date published: 2021-02-17

Can it be uesd to active T cells for proliferation?

Asked by: zhangrunnan
Thank you for your question! Our labs have not tested this antibody clone regarding the possibility to activate T-cell proliferation. However, this clone has been published for the use in functional studies on human Samples. You might be able to find further information here: Chen ML et al. Antipsychotic drugs suppress the AKT/NF-kB pathway and regulate the differentiation of T-cell subsets. Immunol Lett 140:81-91 (2011) If you were to test this antibody in functional studies, we suggest to use this antibody provided in an azide-free (Low endotoxin level) buffer. We can provide this as a special order. If you have any further questions, please contact our Technical Service team. You can find the corresponding contact information, by clicking the following link: https://www.scbt.com/scbt/resources/technical-service
Answered by: Technical Support Europe
Date published: 2019-05-08

Can CD28 (CD28.2): sc-19655 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD28 (CD28.2): sc-19655 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from is greatI ordered for my important data and I so hapy I choose it
Date published: 2017-11-22
Rated 5 out of 5 by from WB detectionmaybe it is not suitable for the intracellular domain CD28.The amino acids sequence is "r skrsrllhsd ymnmtprrpg ptrkhyqpya pprdfaayrs"
Date published: 2017-09-04
Rated 5 out of 5 by from Perfect IF resultPerfect IF result for formalin fixed, paraffin-embedded human kidney tissuse, I suggest 1:200 to 1:300 diluted the antibody.
Date published: 2017-08-26
Rated 5 out of 5 by from ,recommend usePrecise band acquired in WB analysis,recommend use it for 1:1000 dilution.
Date published: 2017-02-26
Rated 4 out of 5 by from frozen sections calf tracheobronchial lymphCD28.2 clone performs well on frozen sections of calf tracheobronchial lymph node. Extra blocking may bring background down a bit more.
Date published: 2017-02-10
Rated 4 out of 5 by from Produced clear immunofluorescence membraneProduced clear immunofluorescence membrane staining in methanol-fixed Jurkat cells. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Strong band seen in WB using Jurkat wholeStrong band seen in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-02-07
Rated 4 out of 5 by from Produced wonderful FCM data for human peripheralProduced wonderful FCM data for human peripheral blood leukocytes. -SCBT QC
Date published: 2014-07-03
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CD28 抗体 (CD28.2) is rated 4.5 out of 5 by 8.
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