Date published: 2025-9-7
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CD24 Antibody (M1/69) - Western Blotting - Image 10633
CD24 抗体 (M1/69): sc-19651. CD24 在 M1 全细胞裂解液 (A), 小鼠胚胎脑 (B) 和小鼠脑 (C) 组织提取物中的表达的 Western 印迹分析.
CD24 抗体 (M1/69): sc-19651. CD24 在 M1 全细胞裂解液 (A), 小鼠胚胎脑 (B) 和小鼠脑 (C) 组织提取物中的表达的 Western 印迹分析.

CD24 抗体 (M1/69): sc-19651

4.2(5)
寫評論提問

说明书
  • CD24 抗体 M1/69 是大鼠单克隆 IgG2b, 在9篇文献中引用，提供 200 µg/ml
  • 针对C57BL/10 mouse脾T细胞和ConA激活的脾细胞
  • CD24 抗体 (M1/69) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测mouse 来源的 CD24
  • 抗CD24抗体（M1/69）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD24 (M1/69): sc-19651的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD24 抗体 (M1/69)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD24抗体（M1/69）是一种鼠单克隆IgG2b抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠来源的CD24蛋白。抗CD24抗体（M1/69）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合型。CD24是一种以糖磷脂酰肌醇（GPI）为锚的膜唾液蛋白，主要表达于前B细胞、前B细胞和成熟B细胞，在B细胞激活后表达显著降低。CD24也存在于粒细胞和一小部分胸腺细胞和神经母细胞瘤中，但在浆细胞中不存在。CD24在调节B细胞增殖和分化方面起着至关重要的作用，是B细胞成熟的标志物。此外，CD24还与各种血液系统恶性肿瘤和多种实体肿瘤有关，其表达可以指示疾病进展。值得注意的是，CD24从细胞顶端定位向细胞质染色转变已被确定为恶性边缘卵巢浆液性肿瘤中基质侵袭的替代标记，这凸显了CD24作为癌症研究生物标志物的潜力。CD24单克隆抗体（M1/69）是研究B细胞生物学和肿瘤免疫学的研究人员的重要工具，有助于深入了解免疫调节和癌症进展的复杂机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD24 抗体 (M1/69)

sc-19651
200 µg/ml
RMB2377.00

CD24 抗体 (M1/69) AC

sc-19651 AC
500 µg/ml, 25% agarose
RMB3129.00

CD24 抗体 (M1/69) HRP

sc-19651 HRP
200 µg/ml
RMB2377.00

CD24 抗体 (M1/69) FITC

sc-19651 FITC
200 µg/ml
RMB2482.00

CD24 抗体 (M1/69) PE

sc-19651 PE
200 µg/ml
RMB2580.00

CD24 抗体 (M1/69) Alexa Fluor® 488

sc-19651 AF488
200 µg/ml
RMB2685.00

CD24 抗体 (M1/69) Alexa Fluor® 546

sc-19651 AF546
200 µg/ml
RMB2685.00

CD24 抗体 (M1/69) Alexa Fluor® 594

sc-19651 AF594
200 µg/ml
RMB2685.00

CD24 抗体 (M1/69) Alexa Fluor® 647

sc-19651 AF647
200 µg/ml
RMB2685.00

CD24 抗体 (M1/69) Alexa Fluor® 680

sc-19651 AF680
200 µg/ml
RMB2685.00

CD24 抗体 (M1/69) Alexa Fluor® 790

sc-19651 AF790
200 µg/ml
RMB2685.00

Has this antibody been tested to act as a blocking antibody?

Asked by: GF1395
Thank you for your question. This antibody is only recommended for detection of CD24 of mouse origin in WB, IP, IF, IHC(P) and FCM assays. It has not been tested for its blocking properties.
Answered by: Tech Support Europe
Date published: 2023-09-10

does this antibody have cross reactivity with human Cd24?

Asked by: ezhil
Thank you for your question. This antibody is only mouse reactive, it is not recommended or warrantied for use with human samples.
Answered by: Tech Service
Date published: 2019-05-15

do you know if this is likely to cross react with hum,an CD24 protein?

Asked by: KarinG
Thank you for your question. We have not validated sc-19651:CD24 (M1/69) for use in human samples. For a limited time, we are offering samples of this antibody, If you are interested in receiving a complimentary sample, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Santa Cruz TS
Date published: 2017-05-19

Can CD24 (M1/69): sc-19651 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD24 (M1/69): sc-19651 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from It worked well in IHCwe used this antibody in IHC 1:50 and worked quite well
Date published: 2022-02-07
Rated 5 out of 5 by from Strong band in WB for mouse thymocyte sampleWe used the antibody in WB for primary mouse thymocyte sample, and we got a strong specific band. It's great!
Date published: 2016-12-20
Rated 2 out of 5 by from Robust bands seen in WB using M1 whole cellRobust bands seen in WB using M1 whole cell lysate and mouse embryo brain and mouse brain tissue extracts. -SCBT QC
Date published: 2015-06-09
Rated 5 out of 5 by from Excellent immunoperoxidase membrane stainingExcellent immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded mouse peripheral blood lymphocytes. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Great FCM data of mouse peripheral bloodGreat FCM data of mouse peripheral blood leukocytes. -SCBT QC
Date published: 2014-11-13
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CD24 抗体 (M1/69) is rated 4.2 out of 5 by 5.
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