Date published: 2026-10-24

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CD20 Antibody (D-10) - Western Blotting - Image 368014
CD20 抗体 (D-10) HRP: sc-393894 HRP. 在 Raji (A), GA-10 (B), BJAB (C), Ramos (D) 和 IB4 (E) 全细胞裂解液以及大鼠脾脏组织提取物 (F) 中 CD20 表达的直接 Western 印迹分析.
CD20 抗体 (D-10) HRP: sc-393894 HRP. 在 Raji (A), GA-10 (B), BJAB (C), Ramos (D) 和 IB4 (E) 全细胞裂解液以及大鼠脾脏组织提取物 (F) 中 CD20 表达的直接 Western 印迹分析.

CD20 抗体 (D-10): sc-393894

4.9(10)
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说明书
  • CD20 抗体 D-10 是小鼠单克隆 IgM κ，CD20抗体, 在20篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的CD20的C-terminus的氨基酸265-290之间
  • 推荐用于 mouse, rat 和 human 来源的CD20 WB, IP, IF, IHC(P) 和 ELISA检测
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • m-IgGκ BP-HRP是CD20 Antibody (D-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与CD20 Antibody (D-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。
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関連項目

CD20抗体（D-10）是一种IgM κ型小鼠单克隆抗体，特定于CD20（也被称为MS4A1抗体、B淋巴细胞抗原CD20抗体或白细胞表面抗原Leu-16抗体）。它能够通过WB, IP, IF, IHC(P)和ELISA检测来自小鼠、大鼠和人类的CD20蛋白质。CD20抗体（D-10）既有非偶联形式的抗CD20抗体，也有多种偶联形式的抗CD20抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。CD20是一种白细胞表面抗原，由四个跨膜区域以及细胞质内的N-和C-末端组成。CD20的细胞质结构域包含多个磷酸化位点，这些位点能够产生不同的异构体。CD20主要在B细胞上表达，但也在正常和肿瘤性T细胞上被发现。CD20作为一种能够通透钙离子的阳离子通道，已知能够加速由胰岛素样生长因子-1（IGF-1）诱导的从G0期到G1期的细胞周期进程。CD20通过异源三聚体G蛋白的α亚基被IGF-1受体激活。CD20的激活显著增加了DNA的合成，并被认为涉及基本的螺旋-环-螺旋亮氨酸拉链转录因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD20 抗体 (D-10) 参考文献:

  1. Ocrelizumab 耗竭多发性硬化症患者的 CD20⁺ T 细胞。  |  Gingele, S., et al. 2018. Cells. 8: PMID: 30597851
  2. 抗CD20免疫抑制疾病改良疗法和新型冠状病毒肺炎。  |  Giovannoni, G. 2020. Mult Scler Relat Disord. 41: 102135. PMID: 32339915
  3. CD3+CD20+ T细胞在进展性多发性硬化症中的细胞毒性特征。  |  Boldrini, VO., et al. 2021. Mult Scler Relat Disord. 52: 103013. PMID: 34030100
  4. CD20+ T细胞作为多发性硬化症的致病因素和治疗靶点。  |  Meinl, E. and Hohlfeld, R. 2021. Ann Neurol. 90: 722-724. PMID: 34585761
  5. 犬CD20作为被动免疫疗法治疗靶标的特征。  |  Dias, JNR., et al. 2022. Sci Rep. 12: 2678. PMID: 35177658
  6. 奥妥珠单抗和奥克珠单抗在皮下或静脉注射后对人源化CD20小鼠的分布和疗效。  |  Torres, JB., et al. 2022. Front Immunol. 13: 814064. PMID: 35967378
  7. 载有阿霉素的抗 CD20 适配体的外泌体选择性抑制人 CD20+ 黑色素瘤干细胞。  |  Chen, H., et al. 2023. Skin Res Technol. 29: e13259. PMID: 36704890
  8. 唾液腺肿瘤中的 CD3 和 CD20 表达及浸润模式。  |  Hussein, RR. and Garib, BT. 2024. Diagnostics (Basel). 14: PMID: 38732373
  9. B-ALL中流式细胞术和免疫组织化学检测的CD20状态不一致率。  |  Grigg, S., et al. 2024. Br J Haematol.. PMID: 39558798
  10. 通过计算表位支架设计水溶性CD20抗原。  |  Yao, Z. and Kuhlman, B. 2024. bioRxiv.. PMID: 39677710

订购信息

产品名称产品编号规格价格数量收藏夹

CD20 抗体 (D-10)

sc-393894
200 µg/ml
CNY2422.00

CD20 (D-10): m-IgGκ BP-HRP 套装

sc-552586
200 µg Ab; 40 µg BP
CNY2715.00

CD20 抗体 (D-10) AC

sc-393894 AC
500 µg/ml, 25% agarose
CNY3189.00

CD20 抗体 (D-10) Alexa Fluor® 488

sc-393894 AF488
200 µg/ml
CNY2738.00

CD20 抗体 (D-10) Alexa Fluor® 647

sc-393894 AF647
200 µg/ml
CNY2738.00

CD20 抗体 (D-10) FITC

sc-393894 FITC
200 µg/ml
CNY2527.00

CD20 抗体 (D-10) HRP

sc-393894 HRP
200 µg/ml
CNY2422.00

CD20 (D-10) 中和勝肽

sc-393894 P
100 µg/0.5 ml
CNY519.00

CD20 抗体 (D-10) PE

sc-393894 PE
200 µg/ml
CNY2625.00

I am looking for CD20 antibodies which expected to work in rat. I am just wondering if I can get a small amount sample to test from you. Thank you so much

Asked by: Anonymous
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email [email protected]
Answered by: BlakeJ
Date published: 2024-03-21

What application is the blocking peptide sc-393894 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by federica15 from Perfect!We have tested a sample of this article. The antibody works perfectly.
Date published: 2019-05-13
Rated 5 out of 5 by xingx from excellent working on paraffin embeded tissue!I asked for some samples to test on HS skin disease and got beautiful staining!
Date published: 2018-07-12
Rated 5 out of 5 by jiapangpang1993 from very wellworks well in a dilution of 1:200 in an IHC experiment.
Date published: 2018-01-08
Rated 5 out of 5 by RRBBH from Good stainingNo problem on human tonsil IHC, demasking pH 9, 1:50 dilution.
Date published: 2017-11-08
Rated 5 out of 5 by Stainer from Excellent for IHC-PI bought this antibody pretty recently and it worked out great in IHC-P formaling fixed tissue in rats.
Date published: 2017-09-14
Rated 5 out of 5 by wyq09 from strong signalWestern blot(dilution 1:200) results; strong signal and low background.
Date published: 2017-05-25
Rated 4 out of 5 by Manuel Flores from Specific stainingThis staining was done on OCT frozen sections of spleen from a 3 weeks CCl4 treated mouse. I used the anti-CD20 Ab in combination with a secondary conjugated to a near-IR fluorescent dye. This combination was useful in reducing autofluorescence. The staining pattern is membranous as expected for a surface marker.
Date published: 2017-03-24
Rated 5 out of 5 by Durantt from works tissueWorks well in western blot with rat spleen tissue, shows a single clear band.
Date published: 2017-02-12
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CD20 抗体 (D-10) is rated 4.9 out of 5 by 10.
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