Date published: 2025-9-6

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CD1D 抗体 (C3D5): sc-19632

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说明书
  • CD1D 抗体 C3D5 是小鼠单克隆 IgG2a κ,CD1D抗体, 在10篇文献中引用,规格为200 µg/ml
  • 针对CD1D的human来源的α1域
  • CD1D 抗体 (C3D5) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 an epitope in the α 1 domain of CD1D
  • 抗CD1D抗体(C3D5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD1D (C3D5): sc-19632免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 CD1D 抗体 (C3D5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与CD1D 抗体 (C3D5) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

CD1D抗体(C3D5)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测人类CD1D蛋白。抗CD1D抗体(C3D5)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CD1多基因家族编码了人类CD1 T细胞表面糖蛋白的5种形式,分别是CD1A、CD1B、CD1C、CD1D和CD1E。CD1D在免疫系统中起着至关重要的作用,它通过向T淋巴细胞呈递脂质抗原来影响T细胞的活化和成熟。CD1D主要位于人体肠上皮细胞表面,已知有两种形式:一种是不依赖于β-2微球蛋白的非糖基化形式,另一种是依赖于β-2微球蛋白的糖基化形式。这些细胞上的CD1D表达有助于识别脂质抗原,这对于针对各种病原体的免疫反应至关重要。此外,CD1D与β-2微球蛋白相关,并表达于皮质胸腺细胞、朗格汉斯细胞、B细胞亚群和某些树突状细胞上,这凸显了其在CD1限制性T细胞发育和激活中的重要性。人类CD1D基因位于1q23.1号染色体上,编码一种335个氨基酸的蛋白质,对T细胞的正常成熟至关重要,因此CD1D(C3D5)单克隆抗体成为研究人员研究免疫反应和T细胞生物学的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD1D 抗体 (C3D5) 参考文献:

  1. 人类 CD1d 在生物合成过程中与脯氨酰-4-羟化酶结合。  |  Kim, HS., et al. 2000. Mol Immunol. 37: 861-8. PMID: 11257307
  2. CD1d 和天然 T 细胞:它们的特性如何启动免疫系统?  |  Joyce, S. 2001. Cell Mol Life Sci. 58: 442-69. PMID: 11315191
  3. 鉴定人类 CD1D 基因编码的 beta 2-微球蛋白结合抗原。  |  Bilsland, CA. and Milstein, C. 1991. Eur J Immunol. 21: 71-8. PMID: 1703966
  4. 第四种 CD1 分子的 cDNA 和编码基因的分离与特征描述。  |  Balk, SP., et al. 1989. Proc Natl Acad Sci U S A. 86: 252-6. PMID: 2463622
  5. 两类 CD1 基因。  |  Calabi, F., et al. 1989. Eur J Immunol. 19: 285-92. PMID: 2467814
  6. 脂质通过肠道CD1d调节外周血清素的释放。  |  Luo, J., et al. 2023. Immunity. 56: 1533-1547.e7. PMID: 37354904
  7. 使用单域抗体增强CD1d磷脂酰丝氨酸的呈现可促进免疫调节性CD1d-TIM-3相互作用。  |  Lameris, R., et al. 2023. J Immunother Cancer. 11: PMID: 38040419
  8. CD1d可防止非酒精性脂肪性肝炎中的肝细胞凋亡。  |  Lei, Z., et al. 2024. J Hepatol. 80: 194-208. PMID: 38438948
  9. CD1 家族:抗原递呈分子的第三系。  |  Porcelli, SA. 1995. Adv Immunol. 59: 1-98. PMID: 7484459
  10. 人类肠上皮细胞表达的不依赖于 MHC Ib 类的β2-微球蛋白分子。  |  Balk, SP., et al. 1994. Science. 265: 259-62. PMID: 7517575
  11. CD1 和 MHC I 类编码分子的抗原呈递。  |  Melián, A., et al. 1996. Curr Opin Immunol. 8: 82-8. PMID: 8729450
  12. 分析人类 CD1 抗原的表达和形成对 beta 2-微球蛋白的要求。  |  Bauer, A., et al. 1997. Eur J Immunol. 27: 1366-73. PMID: 9209486

订购信息

产品名称产品编号规格价格数量收藏夹

CD1D 抗体 (C3D5)

sc-19632
200 µg/ml
RMB2377.00

CD1D (C3D5): m-IgGκ BP-HRP 套装

sc-520694
200 µg Ab, 40 µg BP
RMB2662.00

CD1D 抗体 (C3D5) AC

sc-19632 AC
500 µg/ml, 25% agarose
RMB3129.00

CD1D 抗体 (C3D5) HRP

sc-19632 HRP
200 µg/ml
RMB2377.00

CD1D 抗体 (C3D5) FITC

sc-19632 FITC
200 µg/ml
RMB2482.00

CD1D 抗体 (C3D5) PE

sc-19632 PE
200 µg/ml
RMB2580.00

CD1D 抗体 (C3D5) Alexa Fluor® 488

sc-19632 AF488
200 µg/ml
RMB2685.00

CD1D 抗体 (C3D5) Alexa Fluor® 546

sc-19632 AF546
200 µg/ml
RMB2685.00

CD1D 抗体 (C3D5) Alexa Fluor® 594

sc-19632 AF594
200 µg/ml
RMB2685.00

CD1D 抗体 (C3D5) Alexa Fluor® 647

sc-19632 AF647
200 µg/ml
RMB2685.00

CD1D 抗体 (C3D5) Alexa Fluor® 680

sc-19632 AF680
200 µg/ml
RMB2685.00

CD1D 抗体 (C3D5) Alexa Fluor® 790

sc-19632 AF790
200 µg/ml
RMB2685.00

Can CD1D (C3D5): sc-19632 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD1D (C3D5): sc-19632 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-02-07
Rated 3 out of 5 by from WBThere were two very weak bands in human macrophage samples, and I could not explain the result.
Date published: 2017-01-11
Rated 5 out of 5 by from Published IHC data for use in human carotidPublished IHC data for use in human carotid artery -SCBT Publication Review
Date published: 2015-07-17
Rated 4 out of 5 by from Positive signal produced when using thisPositive signal produced when using this antibody against human CD1D transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from Published data for IP and WB in human HEK293Published data for IP and WB in human HEK293 cells -SCBT QC
Date published: 2015-06-14
Rated 5 out of 5 by from Great antibody for EMSAGreat antibody for EMSA, tested with human THP-1 samples. -SCBT Publication Review
Date published: 2015-06-11
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-06-02
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with the human alveolar epithelial A549 cells and mouse lung carcinoma TC-1 cells -SCBT Publication Review
Date published: 2015-04-29
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CD1D 抗体 (C3D5) is rated 4.4 out of 5 by 10.
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