Date published: 2025-9-8
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CD19 Antibody (B-1) - Western Blotting - Image 287466
CD19 抗体 (B-1): sc-390244. BJAB (A) 和 NAMALWA (B) 全细胞裂解液中 CD19 表达的 Western 印迹分析.
CD19 抗体 (B-1): sc-390244. BJAB (A) 和 NAMALWA (B) 全细胞裂解液中 CD19 表达的 Western 印迹分析.

CD19 抗体 (B-1): sc-390244

3.8(6)
寫評論提問

说明书
  • CD19 抗体 B-1 是小鼠单克隆 IgG2a κ，CD19抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的CD19的N-terminus
  • CD19 抗体 (B-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CD19
  • 抗CD19抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取CD19 (B-1): sc-390244的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是CD19 Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD19 Antibody (B-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD19抗体（B-1）是一种IgG2a κ型小鼠单克隆CD19抗体（也被指定为B淋巴细胞抗原CD19抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的CD19蛋白。CD19抗体（B-1）既有非偶联形式的抗CD19抗体，也有多种偶联形式的抗CD19抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CD19是一种含有两个细胞外免疫球蛋白样结构域的跨膜糖蛋白。CD19选择性表达于B淋巴细胞表面，激活涉及Ras和磷脂酰肌醇3-激酶途径的细胞内信号级联。CD19的激活导致膜蛋白免疫球蛋白链的交联以及与Src家族蛋白酪氨酸激酶（PTK）的后续关联。CD19的表达在B细胞发育过程中持续存在，并通过B细胞最终分化为浆细胞。CD19与B淋巴细胞表面蛋白（包括整合素b1、CD21和CD81）形成功能复合物，参与调节B细胞的发育。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD19 抗体 (B-1)

sc-390244
200 µg/ml
RMB2377.00

CD19 (B-1): m-IgG Fc BP-HRP 套装

sc-530104
200 µg Ab; 10 µg BP
RMB2662.00

CD19 (B-1): m-IgGκ BP-HRP 套装

sc-523462
200 µg Ab, 40 µg BP
RMB2662.00

CD19 (B-1): m-IgG2a BP-HRP 套装

sc-547432
200 µg Ab; 10 µg BP
RMB2662.00

CD19 抗体 (B-1) AC

sc-390244 AC
500 µg/ml, 25% agarose
RMB3129.00

CD19 抗体 (B-1) HRP

sc-390244 HRP
200 µg/ml
RMB2377.00

CD19 抗体 (B-1) FITC

sc-390244 FITC
200 µg/ml
RMB2482.00

CD19 抗体 (B-1) PE

sc-390244 PE
200 µg/ml
RMB2580.00

CD19 抗体 (B-1) Alexa Fluor® 488

sc-390244 AF488
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) Alexa Fluor® 546

sc-390244 AF546
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) Alexa Fluor® 594

sc-390244 AF594
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) Alexa Fluor® 647

sc-390244 AF647
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) Alexa Fluor® 680

sc-390244 AF680
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) Alexa Fluor® 790

sc-390244 AF790
200 µg/ml
RMB2685.00

CD19 抗体 (B-1) B

sc-390244 B
200 µg/ml
RMB2407.00

Can CD19 (B-1): sc-390244 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CD19 (B-1): sc-390244 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from Doesn't workThe antibody doesn't pick up the CD19 in western blot in a human B leukemia cell line extract. 1:100 was the dilution I used. I'm sure the cell expresses CD19 on the surface, and it was confirmed by Flow using two different clones of anti-CD19 antibody.
Date published: 2018-04-30
Rated 5 out of 5 by from Good with IHCWorks fine with IHC (no demasking, 1:50) on human tonsils (5 µm FFPE).
Date published: 2018-04-12
Rated 2 out of 5 by from Unfortunately does not work in cyrosectionsWas promptly delivered but unfortunately does not work in my cryosections of spleen. However, we have had this problem with many CD19 clones so this AB might work very well for another application (e.g.WB, which it was optimised for)
Date published: 2018-01-25
Rated 5 out of 5 by from sc-390244 PE antibodyThis antibody against the CD19 on the cell surface and works very well at 1:100 , strong staining obtained.We recommend use goat serum for blocking buffer.
Date published: 2017-07-29
Rated 5 out of 5 by from Specific results in WBThis monoclonal CD19 Antibody (B-1) gives specific results in Western blot in BJAB, Namwala and U698-M whole cell lysates.
Date published: 2017-02-07
Rated 5 out of 5 by from Produced positive Western blot data of CD19Produced positive Western blot data of CD19 expression in BJAB and NAMALWA whole cell lysates. -SCBT QC
Date published: 2013-04-30
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CD19抗体 (B-1) is rated 3.8 out of 5 by 6.
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