Date published: 2025-9-6

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CD16 抗体 (DJ130c): sc-20052

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说明书
  • CD16 抗体 DJ130c 是小鼠单克隆 IgG1 κ,CD16抗体, 在34篇文献中引用,规格为200 µg/ml
  • 针对CD16
  • CD16 抗体 (DJ130c) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测human 来源的 CD16
  • 抗CD16抗体(DJ130c)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD16 (DJ130c): sc-20052免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 CD16 抗体 (DJ130c) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD16 抗体 (DJ130c) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CD16抗体(DJ130c)是一种小鼠单克隆IgG1 kappa轻链抗体,用于检测人类CD16,也称为Fc gamma受体III(FcγRIII),可用于多种应用,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)。CD16是一种重要的免疫受体,存在两种主要异构体:CD16A(FcγRIIIA)是一种在自然杀伤(NK)细胞和巨噬细胞上表达的跨膜蛋白,CD16B(FcγRIIIB)是一种在嗜中性粒细胞上发现的糖磷脂酰肌醇(GPI)锚定蛋白。这些同源物的独特结构对于其在免疫反应中的特定作用至关重要,CD16A需要与FcεRI的γ亚基或TCR-CD3复合物的ζ亚基结合才能正确表达在细胞表面。这种结构上的多功能性使得CD16能够通过结合IgG抗体的Fc区域来介导抗体依赖性细胞毒(ADCC)和吞噬作用,从而有效地靶向并消灭病原体或受感染的细胞。CD16能够同时作为跨膜受体和GPI锚定受体存在,这凸显了其在免疫调节和反应中的多功能性。CD16单克隆抗体(DJ130c)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®变体,这使得CD16(DJ130c)抗体适用于各种实验应用。抗CD16抗体(DJ130c)对于研究免疫系统动态、自身免疫性疾病以及CD16表达明显改变的疾病(如阵发性夜间血红蛋白尿(PNH))必不可少。CD16(DJ130c)单克隆抗体有多个别名,包括FcγRIII、Fc gamma receptor III、CD16A和CD16B,它具有全面的检测能力,可用于研究免疫系统中CD16的各种结构和功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD16 抗体 (DJ130c) 参考文献:

  1. 膜锚影响 FcgammaRIII (CD16) 与配体结合的二维动力学速率和三维亲和力。  |  Chesla, SE., et al. 2000. J Biol Chem. 275: 10235-46. PMID: 10744709
  2. 与 PI 相连的受体 FcRIII 会在刺激中性粒细胞时释放出来。  |  Huizinga, TW., et al. 1988. Nature. 333: 667-9. PMID: 2967437
  3. 人类自然杀伤细胞上的 IgG Fc 受体:表型, 功能以及与单克隆抗体的比较研究。  |  Perussia, B., et al. 1984. J Immunol. 133: 180-9. PMID: 6233371
  4. 人类中性粒细胞 Fc γ 受体的分布和结构。  |  Fleit, HB., et al. 1982. Proc Natl Acad Sci U S A. 79: 3275-9. PMID: 6808506
  5. CD16(Fc γ 受体 III)异构体的配体结合和吞噬作用。中国仓鼠卵巢细胞中相关zeta和γ亚基的吞噬信号。  |  Nagarajan, S., et al. 1995. J Biol Chem. 270: 25762-70. PMID: 7592758

订购信息

产品名称产品编号规格价格数量收藏夹

CD16 抗体 (DJ130c)

sc-20052
200 µg/ml
RMB2377.00

CD16 (DJ130c): m-IgG Fc BP-HRP 套装

sc-526594
200 µg Ab; 10 µg BP
RMB2662.00

CD16 (DJ130c): m-IgG1 BP-HRP 套装

sc-531967
200 µg Ab; 20 µg BP
RMB2662.00

CD16 抗体 (DJ130c) AC

sc-20052 AC
500 µg/ml, 25% agarose
RMB3129.00

CD16 抗体 (DJ130c) HRP

sc-20052 HRP
200 µg/ml
RMB2377.00

CD16 抗体 (DJ130c) FITC

sc-20052 FITC
200 µg/ml
RMB2482.00

CD16 抗体 (DJ130c) PE

sc-20052 PE
200 µg/ml
RMB2580.00

CD16 抗体 (DJ130c) Alexa Fluor® 488

sc-20052 AF488
200 µg/ml
RMB2685.00

CD16 抗体 (DJ130c) Alexa Fluor® 546

sc-20052 AF546
200 µg/ml
RMB2685.00

CD16 抗体 (DJ130c) Alexa Fluor® 594

sc-20052 AF594
200 µg/ml
RMB2685.00

CD16 抗体 (DJ130c) Alexa Fluor® 647

sc-20052 AF647
200 µg/ml
RMB2685.00

CD16 抗体 (DJ130c) Alexa Fluor® 680

sc-20052 AF680
200 µg/ml
RMB2685.00

CD16 抗体 (DJ130c) Alexa Fluor® 790

sc-20052 AF790
200 µg/ml
RMB2685.00

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Germaine
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 5 out of 5 by from Good for immunostaining HEK cellsThe antibody was good at staining HEK cells that were transiently expressing a construct with CD16.
Date published: 2019-06-05
Rated 5 out of 5 by from immunofluorescent stainingon immunofluorescent staining, this sc-20052 CD16 antibody worked well
Date published: 2019-04-16
Rated 5 out of 5 by from ClearThis antibody works well and used for WB with human platelet extract
Date published: 2017-05-13
Rated 5 out of 5 by from After the use of antibodiesUsed in IHC and got good staining of human spleen tissue at 1:100 dilution.
Date published: 2017-04-24
Rated 5 out of 5 by from WBThere was a weak band at 55 KD in human macrophage lysate by WB
Date published: 2017-04-18
Rated 5 out of 5 by from Superb !!Best of the p16 antibodies;strong western and IHC;much better than polyclonal alternatives
Date published: 2017-01-06
Rated 5 out of 5 by from IHC-PWeak signal could be observed in human macrophages by IHC-P
Date published: 2016-12-25
Rated 5 out of 5 by from Excellent choice for IHC(P) and flowSpecifically stains target in IHC applications in human samples
Date published: 2016-12-23
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CD16 抗体 (DJ130c) is rated 4.7 out of 5 by 17.
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