Date published: 2025-9-6
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CD154 Antibody (F-1) - Western Blotting - Image 154075
CD154 抗体 (F-1): sc-374635. CCRF-CEM (A), HuT 78 (B) 和 SUP-T1 (C) 全细胞裂解液中 CD154 表达的 Western 印迹分析.
CD154 抗体 (F-1): sc-374635. CCRF-CEM (A), HuT 78 (B) 和 SUP-T1 (C) 全细胞裂解液中 CD154 表达的 Western 印迹分析.

CD154 抗体 (F-1): sc-374635

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说明书
  • CD154 抗体 F-1 是小鼠单克隆 IgG2a κ，CD154抗体, 在5篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CD154的C-terminus的氨基酸231-267之间
  • CD154 抗体 (F-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 CD154; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗CD154抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD154 (F-1): sc-374635的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是CD154 Antibody (F-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD154 Antibody (F-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD154抗体（F-1）是一种IgG2a κ小鼠单克隆CD154抗体（也称为CD40LG抗体、CD40L抗体、CD40 Ligand抗体、TNF-Related Activation Protein抗体、TRAP抗体、Tumor Necrosis Factor (Ligand) Superfamily Member 5抗体、T-B Cell-Activating Molecule抗体、CD40 Antigen Ligand抗体、T-Cell Antigen Gp39抗体、TNFSF5抗体、HCD40L抗体、CD40-L抗体、Gp39抗体、HIGM1抗体、T-BAM抗体、IMD3抗体或IGM抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的CD154蛋白。CD154抗体（F-1）既可作为非偶联抗CD154抗体形式存在，也可作为多种偶联形式的抗CD154抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。静息B细胞可以在细胞接触和由初始辅助T（Th）细胞提供的可溶性信号的组合作用下被激活并克隆扩增为抗体产生细胞。虽然细胞因子IL-4和IL-13单独不足以激活B细胞，但与Th细胞的接触似乎足以传递增殖信号。CD40和CD154（也称为CD40L或TRAP）构成了传输此信号的中心受体配体对。CD40表达于B细胞表面，CD154表达于活化T细胞表面。在此类刺激的存在下，IL-4和IL-13能够触发免疫球蛋白类别转换和IgE的分泌。CD154是一种由261个氨基酸组成的蛋白质，它既可以作为可溶性细胞因子表达，也可以作为同型三聚体类型II跨膜蛋白表达。其表达受到严格调控，异常水平的CD154与动脉粥样斑块不稳定性和血栓形成事件的发病机制有关。编码CD154的基因突变与hyper-IgM免疫缺陷综合征1型有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD154 抗体 (F-1) 参考文献:

  1. CD154 和 1 型细胞因子反应：从高 IgM 综合征到人类免疫缺陷病毒感染。  |  Subauste, CS. 2002. J Infect Dis. 185 Suppl 1: S83-9. PMID: 11865444
  2. NFATc1 核占位增强会导致 T 细胞活化，而与 CD28 成本刺激无关。  |  Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
  3. 自发性糖尿病 NOD 小鼠对混合嵌合体诱导的抵抗力取决于 CD40/CD154 途径和供体 MHC 差异。  |  Luo, B., et al. 2007. Ann N Y Acad Sci. 1103: 94-102. PMID: 17376827
  4. CD154 (CD40L)：一种记录对阿莫西林或阿莫西林克拉维酸非即刻过敏性的新型辅助工具。  |  Van Gasse, AL., et al. 2020. Clin Exp Allergy. 50: 640-642. PMID: 32056306
  5. CD154 在缺氧条件下诱导肾小管上皮细胞分泌白细胞介素-6：氯喹的抑制作用  |  Dewitte, A., et al. 2020. Mediators Inflamm. 2020: 6357046. PMID: 32089648
  6. 一种新型 RING 手指蛋白与 CD40 的细胞质结构域相互作用。  |  Hu, HM., et al. 1994. J Biol Chem. 269: 30069-72. PMID: 7527023
  7. CRAF1是TRAF的亲戚，它参与了CD40信号传导。  |  Cheng, G., et al. 1995. Science. 267: 1494-8. PMID: 7533327
  8. 辅助 T 细胞膜激活 B 细胞。  |  Kehry, MR. and Hodgkin, PD. 1994. Crit Rev Immunol. 14: 221-38. PMID: 7538767
  9. CD40 及其配体：B 淋巴细胞生存, 生长和分化的核心角色。  |  Gordon, J. 1995. Blood Rev. 9: 53-6. PMID: 7540904
  10. T 淋巴细胞中 CD40 配体的表达和 IgE 同型转换的诱导。  |  Fuleihan, R., et al. 1995. Int Arch Allergy Immunol. 107: 43-4. PMID: 7542098
  11. TNF 受体 2 和 CD40 介导的 TRAF2 激活 NF-kappa B。  |  Rothe, M., et al. 1995. Science. 269: 1424-7. PMID: 7544915
  12. 与 75 kDa 肿瘤坏死因子受体胞质结构域缔合作用的新型假定信号转导子家族。  |  Rothe, M., et al. 1994. Cell. 78: 681-92. PMID: 8069916

订购信息

产品名称产品编号规格价格数量收藏夹

CD154 抗体 (F-1)

sc-374635
200 µg/ml
RMB2377.00

CD154 (F-1): m-IgG Fc BP-HRP 套装

sc-525983
200 µg Ab; 10 µg BP
RMB2662.00

CD154 (F-1): m-IgG2a BP-HRP 套装

sc-546476
200 µg Ab; 10 µg BP
RMB2662.00

CD154 抗体 (F-1) AC

sc-374635 AC
500 µg/ml, 25% agarose
RMB3129.00

CD154 抗体 (F-1) HRP

sc-374635 HRP
200 µg/ml
RMB2377.00

CD154 抗体 (F-1) FITC

sc-374635 FITC
200 µg/ml
RMB2482.00

CD154 抗体 (F-1) PE

sc-374635 PE
200 µg/ml
RMB2580.00

CD154 抗体 (F-1) Alexa Fluor® 488

sc-374635 AF488
200 µg/ml
RMB2685.00

CD154 抗体 (F-1) Alexa Fluor® 546

sc-374635 AF546
200 µg/ml
RMB2685.00

CD154 抗体 (F-1) Alexa Fluor® 594

sc-374635 AF594
200 µg/ml
RMB2685.00

CD154 抗体 (F-1) Alexa Fluor® 647

sc-374635 AF647
200 µg/ml
RMB2685.00

CD154 抗体 (F-1) Alexa Fluor® 680

sc-374635 AF680
200 µg/ml
RMB2685.00

CD154 抗体 (F-1) Alexa Fluor® 790

sc-374635 AF790
200 µg/ml
RMB2685.00

CD154 (F-1) 中和勝肽

sc-374635 P
100 µg/0.5 ml
RMB511.00

I'm going to do ELISA, I have the antibody now, what else do I need to prepare? Do you have the protocol?

Asked by: XIAONI
Thank you for your question. Here is our ELISA protocol :https://www.scbt.com/resources/protocols/elisa-assays
Answered by: BlakeJ
Date published: 2025-07-23

I'm going to do ELISA, I have the antibody(sc-374635), what else do I need to prepare? Do you have protocol?

Asked by: XIAONI
Thank you for your question. Here is our ELISA protocol :https://www.scbt.com/resources/protocols/elisa-assays
Answered by: BlakeJ
Date published: 2025-07-23

Does CD154 Antibody (F-1) works with Rhesus CD40L WB?

Asked by: Narayana
Thank you for your question. This antibody has not been tested for Rhesus samples, however, based on epitope sequence homology, it should be fine for use with monkey samples.
Answered by: Tech Service
Date published: 2019-05-22

Do you have a sample staining image for rat CD154?

Asked by: Alex Hartlage
This primary antibody is recommended and warrantied for mouse, rat and human samples, for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-26

What application is the blocking peptide sc-374635 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Works for WBOrdered the conjugated Ab but was sent the unconjugated version. I had to use a secondary but the antibody worked nicely and showed little background.
Date published: 2018-09-26
Rated 2 out of 5 by from did work but not very specificstaining not very specific in human PFA-fixed paraffin-embedded retina sections, and pattern varies A LOT......
Date published: 2018-02-15
Rated 5 out of 5 by from Produced positive Western Blot data of humanProduced positive Western Blot data of human recombinant CD154. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Nice Western Blot data of CD154 expression in CCRFNice Western Blot data of CD154 expression in CCRF-CEM, HuT 78 and SUP-T1 whole cell lysates. -SCBT QC
Date published: 2014-01-17
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CD154 抗体 (F-1) is rated 4.0 out of 5 by 4.
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