Date published: 2025-9-7
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CD10 Antibody (F-4) - Western Blotting - Image 344727
CD10 抗体 (F-4): sc-46656. 拉莫斯 (A) 和拉吉 (B) 全细胞裂解液及人肾组织提取物 (C) 中 CD10 表达的 Western 印迹分析.
CD10 抗体 (F-4): sc-46656. 拉莫斯 (A) 和拉吉 (B) 全细胞裂解液及人肾组织提取物 (C) 中 CD10 表达的 Western 印迹分析.

CD10 抗体 (F-4): sc-46656

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说明书
  • CD10 抗体 F-4 是小鼠单克隆 IgG1 κ，CD10抗体, 在30篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD10的氨基酸230-550
  • CD10 抗体 (F-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 CD10
  • 抗CD10抗体（F-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD10 (F-4): sc-46656的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 CD10 抗体 (F-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD10 抗体 (F-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

CD10 and Neprilysin 抗体

CD10抗体（F-4）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测鼠、鼠和人源CD10蛋白。CD10 (F-4) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CD10，也称为常见急性淋巴细胞白血病抗原（CALLA）和中性内切肽酶（NEP），是一种II型整合膜糖蛋白，在调节细胞外环境中的肽水平方面起着至关重要的作用。作为一种锌金属蛋白酶，CD10可分解多种生物活性肽，包括血管紧张素I和II，它们对于血压调节和体液平衡至关重要。CD10在早期B和T淋巴细胞前体、B细胞、某些粒细胞、骨髓基质细胞和特定上皮细胞（包括一些肿瘤细胞系）上均有表达。CD10作为常见急性淋巴细胞白血病和某些淋巴瘤的标志物，在诊断和治疗方面具有重要意义。抗CD10抗体（F-4）是研究人员研究血液系统恶性肿瘤和肽调节在各种生理过程中的作用的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD10 抗体 (F-4) 参考文献:

  1. CD10+ 毛细胞白血病分析。  |  Jasionowski, TM., et al. 2003. Am J Clin Pathol. 120: 228-35. PMID: 12931553
  2. CD10 表达对浸润性乳腺癌临床预后的影响  |  Vo, TN., et al. 2015. Breast Cancer. 22: 117-28. PMID: 23575921
  3. 皮肤基底细胞癌和鳞状细胞癌中 CD10 的免疫组化表达。  |  Shafaei, S., et al. 2015. Caspian J Intern Med. 6: 103-7. PMID: 26221509
  4. 乳腺 Apocrine 病变中的 CD10 免疫组化表达  |  Kővári, B., et al. 2015. Pathobiology. 82: 259-63. PMID: 26562027
  5. 针对人类白细胞抗原的单克隆抗体。II.针对 CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), 转铁蛋白受体 (T9), 一种新型广谱表达的 18 kDa 抗原 (MEM-43) 和一种新型限制表达抗原 (MEM-74) 的抗体。  |  Horejsí, V., et al. 1988. Folia Biol (Praha). 34: 23-34. PMID: 2968928
  6. 评估作为结直肠癌诊断标志物的 CD10 表达。  |  Żurawski, J., et al. 2022. Gastroenterol Hepatol Bed Bench. 15: 24-31. PMID: 35611259
  7. CD10 阳性套细胞淋巴瘤的临床病理特征和治疗：病例报告和文献综述。  |  Hino, C., et al. 2022. Pathol Oncol Res. 28: 1610588. PMID: 36091937
  8. FAP、CD10和GPR77标记的CAFs通过诱导胃癌的EMT和CSC导致新辅助化疗耐药。  |  Zhao, Z. and Zhu, Y. 2023. BMC Cancer. 23: 507. PMID: 37277751
  9. 人和小鼠的 CD10（NEP 24.11，CALLA）在非淋巴器官中的分布相似，但在造血系统中却有所不同：小鼠的 T 和 B 淋巴祖细胞上没有 CD10。  |  Kalled, SL., et al. 1995. Eur J Immunol. 25: 677-87. PMID: 7705396
  10. 中性内肽酶对脓毒性休克的调节作用。  |  Lu, B., et al. 1995. J Exp Med. 181: 2271-5. PMID: 7760013
  11. 作为膜相关酶的造血分化抗原：切割是关键！  |  Shipp, MA. and Look, AT. 1993. Blood. 82: 1052-70. PMID: 8102558
  12. 急性白血病中的 CD10。GEIL（白血病免疫学研究小组）。  |  Béné, MC. and Faure, GC. 1997. Haematologica. 82: 205-10. PMID: 9175328

订购信息

产品名称产品编号规格价格数量收藏夹

CD10 抗体 (F-4)

sc-46656
200 µg/ml
RMB2377.00

CD10 (F-4): m-IgG Fc BP-HRP 套装

sc-526715
200 µg Ab; 10 µg BP
RMB2662.00

CD10 (F-4): m-IgG1 BP-HRP 套装

sc-532088
200 µg Ab; 20 µg BP
RMB2662.00

CD10 抗体 (F-4) AC

sc-46656 AC
500 µg/ml, 25% agarose
RMB3129.00

CD10 抗体 (F-4) HRP

sc-46656 HRP
200 µg/ml
RMB2377.00

CD10 抗体 (F-4) FITC

sc-46656 FITC
200 µg/ml
RMB2482.00

CD10 抗体 (F-4) PE

sc-46656 PE
200 µg/ml
RMB2580.00

CD10 抗体 (F-4) Alexa Fluor® 488

sc-46656 AF488
200 µg/ml
RMB2685.00

CD10 抗体 (F-4) Alexa Fluor® 546

sc-46656 AF546
200 µg/ml
RMB2685.00

CD10 抗体 (F-4) Alexa Fluor® 594

sc-46656 AF594
200 µg/ml
RMB2685.00

CD10 抗体 (F-4) Alexa Fluor® 647

sc-46656 AF647
200 µg/ml
RMB2685.00

CD10 抗体 (F-4) Alexa Fluor® 680

sc-46656 AF680
200 µg/ml
RMB2685.00

CD10 抗体 (F-4) Alexa Fluor® 790

sc-46656 AF790
200 µg/ml
RMB2685.00

Hello, I am interested in CD10 Antibody (F-4): sc-46656. But I need to test if it works on our exosomal preps. Can I get any sample size of that antibody? Please let me know that. Thanks.

Asked by: yoaan
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-11-18

What is your suggested secondary for this mouse monoclonal CD10 Antibody (F-4): sc-46656?

Asked by: Anna99
We recommend using one our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-17
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Rated 5 out of 5 by from Great featuresI will recommend these antibodies. Clear band at 1:500 dilution, whole cell lysate of colon cancer cells.
Date published: 2018-07-13
Rated 5 out of 5 by from Very clear and theWe found a clear result when it's 1:800 at WB and the work was beautiful and satisfying.
Date published: 2017-10-27
Rated 5 out of 5 by from the result satisfiedCD10 Antibody works well in whole cell lysates and the result is abvious. High specificity, sensitivity, convenience and quickness both the advantages of the antibody.
Date published: 2017-10-22
Rated 4 out of 5 by from Good antibody!The antibody works well on our mouse brain lysates. The bands are clear (although not so strong and need longer exposure) and at right position.
Date published: 2017-09-26
Rated 5 out of 5 by from recommend use by WBGood surface marker for mouse kidney tissue detecion by WB,recommend use blocking buffer (5% non-fat milk for dilution), 1:500 dilution.Just very fine band and specific at 100kda.
Date published: 2017-08-26
Rated 5 out of 5 by from CD10 (F-4)Good clear band at 100 kDa in Ramos whole cell lysates.
Date published: 2016-12-20
Rated 5 out of 5 by from Very nice antibody.I tried this antibody on mouse liver samples with immunofluorescence staining which can work well.
Date published: 2016-12-13
Rated 5 out of 5 by from value for moneyI bought it last week and it is really good value for money. It helps quite a lot in my work. recommend～
Date published: 2016-11-30
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CD10 抗体 (F-4) is rated 4.9 out of 5 by 12.
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