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Cbl-b Antibody (G-1) - Western Blotting - Image 397521 — Cbl-b 抗体 (G-1): sc-8006. BJAB (A), NAMALWA (B), Jurkat (C), CTLL-2 (D) 和 TK-1 (E) 全细胞裂解液中 Cbl-b 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Cbl-b 抗体 (G-1): sc-8006. BJAB (A), NAMALWA (B), Jurkat (C), CTLL-2 (D) 和 TK-1 (E) 全细胞裂解液中 Cbl-b 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

Cbl-b 抗体 (G-1): sc-8006

Name: Cbl-b抗体 (G-1)
Brand: SCBIO
SKU: sc-8006
Price: 2377.0 CNY
Availability: InStock

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说明书

Cbl-b 抗体 G-1 是小鼠单克隆 IgG₁ κ，Cbl-b抗体, 在86篇文献中引用，规格为200 µg/ml
免疫human来源Cbl-b位于N-terminus 的29-483氨基酸
Cbl-b 抗体 (G-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cbl-b
抗Cbl-b抗体（G-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Cbl-b (G-1): sc-8006的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 Cbl-b 抗体 (G-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cbl-b 抗体 (G-1) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(85)

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Cbl-b 抗体 (G-1) 参考文献:

人类和小鼠c-cbl原癌基因的序列显示，v-cbl是由一个包含富脯氨酸结构域和一个类似亮氨酸拉链的基序的大截短产生的。 | Blake, TJ., et al. 1991. Oncogene. 6: 653-7. PMID: 2030914
c-cbl 原癌基因主要在胸腺和睾丸组织中表达，编码一种核蛋白。 | Langdon, WY., et al. 1989. J Virol. 63: 5420-4. PMID: 2585608
鉴定两个与 v-cbl 肿瘤基因同源的鼠类基因座。 | Regnier, DC., et al. 1989. J Virol. 63: 3678-82. PMID: 2760978
v-cbl，一种来自双重组小鼠逆转录病毒的致癌基因，可诱导早期 B 线淋巴瘤。 | Langdon, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 1168-72. PMID: 2784003
RNF125 和 Cbl-b 对 NLRP3 的顺序泛素化限制了炎性体的激活和内毒素血症。 | Tang, J., et al. 2020. J Exp Med. 217: PMID: 31999304
泛素连接酶 Cbl-b 和抑制性 Cblin 肽。 | Nikawa, T. and Ishidoh, K. 2020. Biochim Biophys Acta Proteins Proteom. 1868: 140495. PMID: 32663526
缺失Cbl-b可抑制CD8+ T细胞衰竭并促进CAR T细胞功能。 | Kumar, J., et al. 2021. J Immunother Cancer. 9: PMID: 33462140
靶向Cbl-b进行癌症免疫治疗。 | Augustin, RC., et al. 2023. J Immunother Cancer. 11: PMID: 36750253
Cbl和Cbl-b通过不同的受体相互作用模式独立调节表皮生长因子受体。 | Pinilla-Macua, I. and Sorkin, A. 2023. Mol Biol Cell. 34: ar134. PMID: 37903221
CBL-B--即将到来的免疫肿瘤学靶标。 | Fusco, R., et al. 2025. Expert Opin Ther Pat. 35: 47-64. PMID: 39582379
克隆和鉴定 cbl-b：与 c-cbl 原癌基因具有同源性的 SH3 结合蛋白。 | Keane, MM., et al. 1995. Oncogene. 10: 2367-77. PMID: 7784085
通过与 p56lck 无关的机制在淋巴细胞膜上形成 c-Cbl.磷脂酰肌醇 3- 激酶复合物。 | Hartley, D. and Corvera, S. 1996. J Biol Chem. 271: 21939-43. PMID: 8702998

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Cbl-b 抗体 (G-1)	sc-8006	200 µg/ml	RMB2377.00
Cbl-b (G-1): m-IgG Fc BP-HRP 套装	sc-528197	200 µg Ab; 10 µg BP	RMB2662.00
Cbl-b (G-1): m-IgGκ BP-HRP 套装	sc-520519	200 µg Ab, 40 µg BP	RMB2662.00
Cbl-b (G-1): m-IgG₁ BP-HRP 套装	sc-542810	200 µg Ab; 20 µg BP	RMB2662.00
Cbl-b 抗体 (G-1) AC	sc-8006 AC	500 µg/ml, 25% agarose	RMB3129.00
Cbl-b 抗体 (G-1) HRP	sc-8006 HRP	200 µg/ml	RMB2377.00
Cbl-b 抗体 (G-1) FITC	sc-8006 FITC	200 µg/ml	RMB2482.00
Cbl-b 抗体 (G-1) PE	sc-8006 PE	200 µg/ml	RMB2580.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 488	sc-8006 AF488	200 µg/ml	RMB2685.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 546	sc-8006 AF546	200 µg/ml	RMB2685.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 594	sc-8006 AF594	200 µg/ml	RMB2685.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 647	sc-8006 AF647	200 µg/ml	RMB2685.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 680	sc-8006 AF680	200 µg/ml	RMB2685.00
Cbl-b 抗体 (G-1) Alexa Fluor^® 790	sc-8006 AF790	200 µg/ml	RMB2685.00

Since it's marked for use for IP, I tried doing the IP using this G1 antibody followed by Western Blot for pTyrosine (PY20) and it didn't work whereas it has worked in reverse IP using PY20 followed by western blot for Cbl-b. Any advice? 

Asked by: LeilaniC

Thank you for your question. Cbl-b Antibody (G-1): sc-8006 is recommended for immunoprecipitation of Cbl-b. It would depend on your samples whether or not you could detect phosphorylated tyrosine by Western blot, following immunoprecipitation of Cbl.b. We would suggest doing a Western blot for Cbl-b in your samples before, and after IP with the (G-1) clone to determine whether or not the antibody is working for enrichment of Cbl-b. If you still have questions, please contact our Technical Service Department and we would be happy to help you troubleshoot these antibodies. Please call us toll-free at (800) 457-3801 option 2, or e-mail [email protected].

Answered by: Technical Support

Date published: 2017-04-06

Hello, i have been working with C-20 antibody against Cbl-b in Jurkat cells for IP in the last years, and it worked very good (an excellent ab). But now, i have tested G-1 antibody and it didn't work, even with Cblb-overexpressing Jurkat. Any advice?

Asked by: Javier Robles

Thank you for your question. We are sorry to hear that this antibody did not perform for you as expected. We want you to be successful in your research. Santa Cruz Biotechnology does warrant our antibodies for one year from the date of purchase. Please contact our Technical Service Department ([email protected]). We are happy to help you to troubleshoot the experiment.

Answered by: Technical Support Europe

Date published: 2017-01-31

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt

Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.

Answered by: Tech Service 9

Date published: 2017-01-04

Rated 5 out of 5 by monti from Nice productWorks well in primary mouse T cells and breast cancer cell lines in Western Blot and Immunoprecipitation. Consider using a low dilution to improve results (1:100)

Date published: 2021-06-21

Rated 4 out of 5 by Virginie from worked well in western blotThis antibody worked well in western blot on mouse osteoclasts extract.

Date published: 2017-10-25

Rated 5 out of 5 by hoomy from Good bandGood band seen in WB using human recombinant Cbl-b.

Date published: 2017-05-07

Rated 5 out of 5 by jenniferc from Great WB antibodyProduced specific results. Validated specificity with siRNA knockout. Highly recommended!

Date published: 2017-01-10

Rated 5 out of 5 by Bill28 from Great antibodyVery clear strong western data; low background;nice si rna control

Date published: 2016-12-31

Rated 1 out of 5 by Medchem from No detectable expression in ARPE-19 cellsNo detectable expression in ARPE-19 cells after reaching confluence. Used 1:500 dilution, observed just non-specific bands.

Date published: 2016-09-26

Rated 5 out of 5 by LM from Published in IF for paraffinPublished in IF for paraffin-embedded human T cells. -SCBT Publication Review

Date published: 2015-05-11

Rated 4 out of 5 by HF from Great data published on Western blot on mouse 3T3Great data published on Western blot on mouse 3T3-L1 adipocytes. -SCBT Publication Review

Date published: 2015-04-25

Cbl-b 抗体 (G-1) Reviews - page 2

Cbl-b 抗体 (G-1) is rated 4.4 out of 5 by 17.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	CBLB	Entrez Gene ID: 868	3q13.11	XM_011513257, XM_011513259, XM_017007395, XM_017007396, XM_017007397, XM_017007398, XM_017007399, XM_017007400, NM_001321786, NM_001321788, NM_001321789, NM_001321790, NM_001321791, NM_001321793, NM_001321794, NM_001321795, NM_001321796, NM_001321797, NM_001321798, NM_001321799, NM_001321806, NM_001321807, NM_001321808, NM_001321811, NM_001321813, NM_001321816, NM_001321820, NM_001321822, NM_170662	UniProt ID: Q13191	604491
Mouse	Cblb	Entrez Gene ID: 208650	16 B5	XM_006521947, XM_006521948, XM_006521949, XM_006521950, XM_006521951, XM_006521952, NM_001033238	UniProt ID: Q3TTA7	N/A

Cbl-b 抗体 (G-1): sc-8006

Cbl-b 抗体 (G-1) 参考文献:

Cbl-b 抗体 (G-1) 规格

替代产品名称: CBLB

产品克隆名称: G-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 29-483

抗原表位靶标: Cbl-b

抗原表位种属: human

产品反应性/检测: Cbl-b

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 86篇各种科学出版物的引文

Cbl-b 抗体 (G-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Cbl-b 抗體是 Cbl-b 抗體 (B-5): sc-376409.

Cbl-b 抗体 (G-1)

Cbl-b (G-1): m-IgG Fc BP-HRP 套装

Cbl-b (G-1): m-IgGκ BP-HRP 套装

Cbl-b (G-1): m-IgG₁ BP-HRP 套装

Cbl-b 抗体 (G-1) AC

Cbl-b 抗体 (G-1) HRP

Cbl-b 抗体 (G-1) FITC

Cbl-b 抗体 (G-1) PE

Cbl-b 抗体 (G-1) Alexa Fluor^® 488

Cbl-b 抗体 (G-1) Alexa Fluor^® 546

Cbl-b 抗体 (G-1) Alexa Fluor^® 594

Cbl-b 抗体 (G-1) Alexa Fluor^® 647

Cbl-b 抗体 (G-1) Alexa Fluor^® 680

Cbl-b 抗体 (G-1) Alexa Fluor^® 790

Since it's marked for use for IP, I tried doing the IP using this G1 antibody followed by Western Blot for pTyrosine (PY20) and it didn't work whereas it has worked in reverse IP using PY20 followed by western blot for Cbl-b. Any advice?

Hello, i have been working with C-20 antibody against Cbl-b in Jurkat cells for IP in the last years, and it worked very good (an excellent ab). But now, i have tested G-1 antibody and it didn't work, even with Cblb-overexpressing Jurkat. Any advice?

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Cbl-b 抗体 (G-1): sc-8006

Cbl-b 抗体 (G-1) 参考文献:

Cbl-b 抗体 (G-1) 规格

替代产品名称: CBLB

产品克隆名称: G-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 29-483

抗原表位靶标: Cbl-b

抗原表位种属: human

产品反应性/检测: Cbl-b

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 86篇各种科学出版物的引文

Cbl-b 抗体 (G-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Cbl-b 抗體是 Cbl-b 抗體 (B-5): sc-376409.

Cbl-b 抗体 (G-1)

Cbl-b (G-1): m-IgG Fc BP-HRP 套装

Cbl-b (G-1): m-IgGκ BP-HRP 套装

Cbl-b (G-1): m-IgG1 BP-HRP 套装

Cbl-b 抗体 (G-1) AC

Cbl-b 抗体 (G-1) HRP

Cbl-b 抗体 (G-1) FITC

Cbl-b 抗体 (G-1) PE

Cbl-b 抗体 (G-1) Alexa Fluor® 488

Cbl-b 抗体 (G-1) Alexa Fluor® 546

Cbl-b 抗体 (G-1) Alexa Fluor® 594

Cbl-b 抗体 (G-1) Alexa Fluor® 647

Cbl-b 抗体 (G-1) Alexa Fluor® 680

Cbl-b 抗体 (G-1) Alexa Fluor® 790

Since it's marked for use for IP, I tried doing the IP using this G1 antibody followed by Western Blot for pTyrosine (PY20) and it didn't work whereas it has worked in reverse IP using PY20 followed by western blot for Cbl-b. Any advice?&nbsp;

Hello, i have been working with C-20 antibody against Cbl-b in Jurkat cells for IP in the last years, and it worked very good (an excellent ab). But now, i have tested G-1 antibody and it didn't work, even with Cblb-overexpressing Jurkat. Any advice?

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Cbl-b 抗体 (G-1)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

Cbl-b (G-1): m-IgG₁ BP-HRP 套装

Cbl-b 抗体 (G-1) Alexa Fluor^® 488

Cbl-b 抗体 (G-1) Alexa Fluor^® 546

Cbl-b 抗体 (G-1) Alexa Fluor^® 594

Cbl-b 抗体 (G-1) Alexa Fluor^® 647

Cbl-b 抗体 (G-1) Alexa Fluor^® 680

Cbl-b 抗体 (G-1) Alexa Fluor^® 790

Since it's marked for use for IP, I tried doing the IP using this G1 antibody followed by Western Blot for pTyrosine (PY20) and it didn't work whereas it has worked in reverse IP using PY20 followed by western blot for Cbl-b. Any advice?