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CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) - Western Blotting - Image 352105
CB1 抗体 (2F9): sc-293419. 大鼠睾丸组织提取物中 CB1 表达的 Western 印迹分析.
CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) - Western Blotting - Image 352105
CB1 抗体 (2F9): sc-293419. 大鼠睾丸组织提取物中 CB1 表达的 Western 印迹分析.
CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) - Western Blotting - Image 348457
CB1 抗体 (2F9): sc-293419. 人重组 CB1 融合蛋白的 Western 印迹分析.
CB1 抗体 (2F9): sc-293419. 大鼠睾丸组织提取物中 CB1 表达的 Western 印迹分析.

CB1/Cannabinoid Receptor 1/CNR1 抗体 (2F9): sc-293419

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说明书
  • CB1/Cannabinoid Receptor 1/CNR1 抗体 2F9 是小鼠单克隆 IgG2b κ，CB1/Cannabinoid Receptor 1/CNR1抗体, 在13篇文献中引用，规格为100 µg/ml
  • 针对氨基酸1-110，代表重组部分长度CB1，源自human
  • 推荐用于 mouse, rat 和 human 来源的CB1 WB, IP 和 ELISA检测
  • 关于如何获取CB1/Cannabinoid Receptor 1/CNR1 (2F9): sc-293419的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与CB1/Cannabinoid Receptor 1/CNR1 Antibody (2F9) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

CB1抗体（2F9）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的CB1蛋白。抗CB1抗体（2F9）以非结合形式提供。CB1，也称为大麻素受体1，是一种G蛋白偶联受体，主要表达于大脑，在调节神经递质释放和影响各种生理过程方面发挥着关键作用。CB1在中枢神经系统的定位尤为重要，因为它参与调节疼痛、情绪、食欲和记忆，因此CB1成为治疗慢性疼痛、焦虑和神经退行性疾病的重要靶点。CB1会经历翻译后修饰，包括磷酸化，这会影响CB1的活性及其与其他蛋白的相互作用。丝氨酸316的磷酸化会破坏CB1介导的钙通道抑制，从而影响神经元的兴奋性和信号通路。这种复杂的调节机制凸显了CB1在维持神经系统内稳态方面的重要性，并凸显了CB1作为药物开发靶标的潜力，旨在调节大麻素效应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CB1/Cannabinoid Receptor 1/CNR1 抗体 (2F9) 参考文献:

  1. 2-arachidonoylglycerol 而非 N-棕榈酰乙醇胺或 anandamide 是大麻素 CB2 受体生理配体的证据。比较各种大麻素受体配体在 HL-60 细胞中的激动活性。  |  Sugiura, T., et al. 2000. J Biol Chem. 275: 605-12. PMID: 10617657
  2. 内源性大麻素系统与大脑发育。  |  Fernández-Ruiz, J., et al. 2000. Trends Neurosci. 23: 14-20. PMID: 10631784
  3. 缺乏 CB1 受体的小鼠应激诱导的镇痛作用减弱，但外源性阿片类药物的作用并未减弱。  |  Valverde, O., et al. 2000. Eur J Neurosci. 12: 533-9. PMID: 10712632
  4. 分子克隆出一种也在睾丸中表达的人类大麻素受体。  |  Gérard, CM., et al. 1991. Biochem J. 279 (Pt 1): 129-34. PMID: 1718258
  5. 用于人体大脑中1型大麻素受体（CB1）体内成像的放射性配体的开发。  |  Horti, AG. and Van Laere, K. 2008. Curr Pharm Des. 14: 3363-83. PMID: 19075713
  6. CB1大麻素受体的信号传导。  |  Turu, G. and Hunyady, L. 2010. J Mol Endocrinol. 44: 75-85. PMID: 19620237
  7. 大麻素受体的结构和克隆 cDNA 的功能表达。  |  Matsuda, LA., et al. 1990. Nature. 346: 561-4. PMID: 2165569
  8. 儿茶酚胺能神经元中大麻素CB1受体的缺失可预防饮食诱导的肥胖。  |  Srivastava, RK., et al. 2022. Int J Mol Sci. 23: PMID: 36293486
  9. 大麻素外周受体的分子特征。  |  Munro, S., et al. 1993. Nature. 365: 61-5. PMID: 7689702
  10. 小鼠 CB2 外周大麻素受体的分子克隆, 表达和功能。  |  Shire, D., et al. 1996. Biochim Biophys Acta. 1307: 132-6. PMID: 8679694
  11. 蛋白激酶 C 通过磷酸化 CB1 大麻受体破坏大麻素作用。  |  Garcia, DE., et al. 1998. J Neurosci. 18: 2834-41. PMID: 9526000
  12. CB1 受体基因敲除小鼠对大麻素反应迟钝，鸦片制剂的成瘾效应降低。  |  Ledent, C., et al. 1999. Science. 283: 401-4. PMID: 9888857

订购信息

产品名称产品编号规格价格数量收藏夹

CB1/Cannabinoid Receptor 1/CNR1 抗体 (2F9)

sc-293419
100 µg/ml
$316.00

CB1/Cannabinoid Receptor 1/CNR1 (2F9): m-IgG Fc BP-HRP 套装

sc-540290
100 µg Ab; 10 µg BP
$354.00

CB1/Cannabinoid Receptor 1/CNR1 (2F9): m-IgG2b BP-HRP 套装

sc-549784
100 µg Ab; 10 µg BP
$354.00

Hello, I need to order CB1 and CB2 antibodies. Do you also provide positive and negative samples? I need the antibodies doing immunohistochemistry (IHC). Would the antibodies work for IHC?

Asked by: Farideh
Thank you for your question. We do not offer positive and negative tissue samples for IHC. This antibody is not currently recommended for IHC. We do offer an alternative, CB1 Antibody (C-11): sc-518035. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. For CB2, we offer the following, CB2 Antibody (3C7): sc-293188. Please note that neither one of these CB1 or CB2 antibodies has been tested yet on formalin-fixed paraffin-embedded tissues sections, and so IHC(P) is not yet a recommended application. Please check back for updates!
Answered by: Technical Support
Date published: 2019-02-02

For Western Blot, is it recommended to use denatured or non-denatured conditions with CB1 (2F9): sc-293419 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2021-06-29
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Rated 4 out of 5 by from Very GoodWe have tested a free sample and we have obtained very good results by Western blotting, with homogenates of rat hippocampus, using 1:500 dilution.
Date published: 2022-11-03
Rated 4 out of 5 by from Tested for WBIt works Ok for WB. I had to adjust the concentrations, best results with dilution at 1:500.
Date published: 2022-03-15
Rated 4 out of 5 by from antibody works wellThis antibody works well, but the molecular weight of CB1 does not show up at 50 kDa as the figure has indicated. CB1 is highly glycosylated and it appears at around 65 kDa although the theoretical molecular weight of CB1 is around 50 kDa. See this paper: J Neurochem. 2017, 142(4):521-533
Date published: 2021-10-23
Rated 4 out of 5 by from IHC and immunofluorescence for huan brain tissuesIHC for human brain tissues, which were formalin-fixed, paraffin-embedded. Brown color is CB1R. Immunofluorescence for human brain tissues, which were formalin-fixed, paraffin-embedded. Red color is CB1R, green color is Iba1(microglia cell maker).
Date published: 2018-09-23
Rated 5 out of 5 by from much better than beforework well in lysate from mouse brain cortex with a single specific band at expected mw ( detected additional band at 42 kD, not tested for possible upper bands),although we got not so strong signal considering the receptor brain expression
Date published: 2018-04-13
Rated 1 out of 5 by from Not working for Brain tissue ImmunoHistologyTried the Sample test for Fluorescence ImmunoHistology -Brain perfused with 4% PFA, transcardially -non-embedded free-floting section (40um) -"Antigene retrieval" at 80° in 10mM NatriumCitrate -PBS buffer -Concentration 1:100---Not tried for WB
Date published: 2018-01-04
Rated 5 out of 5 by from High specificity with dilutions around 1:400This antibody works well in Western blot (1:400) for mouse brain in alcoholism model
Date published: 2017-12-25
Rated 5 out of 5 by from High specificity with dilutions around 1:200This antibody works well in Western blot (1:200) and immunohistochemistry (1:100) for placental explants
Date published: 2017-05-09
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CB1/Cannabinoid Receptor 1/CNR1 抗体 (2F9) is rated 4.0 out of 5 by 8.
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