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cathepsin L Antibody (G-11) - Western Blotting - Image 355317 — cathepsin L 抗体 (G-11): sc-390367. PMJ2-PC (A), F9 (B) 和 RPE-J (C) 全细胞裂解液中 cathepsin L 表达的 Western 印迹分析.

cathepsin L 抗体 (G-11): sc-390367. PMJ2-PC (A), F9 (B) 和 RPE-J (C) 全细胞裂解液中 cathepsin L 表达的 Western 印迹分析.

cathepsin L 抗体 (G-11): sc-390367

Name: cathepsin L抗体 (G-11)
Brand: SCBIO
SKU: sc-390367
Price: 2377.0 CNY
Availability: InStock

3.5(2)

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说明书

cathepsin L抗体(G-11)是小鼠单克隆IgG_2a κ, 在7篇文献中引用，规格为200 µg/ml
特异性抗原位于mouse物种的cathepsin L的C-terminus的氨基酸263-291之间
抗-cathepsin L 抗体 (G-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse 和rat 来源的 cathepsin L
抗cathepsin L抗体（G-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取cathepsin L (G-11): sc-390367的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG_2a BP-HRP是cathepsin L Antibody (G-11) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与cathepsin L Antibody (G-11) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(7)

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関連項目

额外Cathepsin抗体，包括cathepsin 6, cathepsin A, cathepsin B, cathepsin C, cathepsin D, cathepsin E, cathepsin H, cathepsin K, cathepsin L, cathepsin O, cathepsin S, cathepsin V, cathepsin W, cathepsin Z和pan cathepsin

cathepsin L抗体（G-11）是一种IgG2a κ小鼠单克隆cathepsin L抗体（也称作cathepsin L抗体或CTSL抗体），该抗体能通过WB、IP、IF和ELISA等方式检测小鼠和大鼠来源的cathepsin L。cathepsin L抗体（G-11）既可作为非共轭抗cathepsin L抗体形式存在，也可作为多种共轭形式的抗cathepsin L抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。蛋白酶家族的蛋白酶包含几个不同的类别。半胱氨酸蛋白酶类包括cathepsins B、L、H、K、S和O。天冬氨酸蛋白酶类由cathepsins D和E组成。Cathepsin G属于丝氨酸蛋白酶类。大多数组织蛋白酶都是溶酶体的，每一种都参与细胞代谢，参与各种事件，如肽的生物合成和蛋白质降解。Cathepsin L（也称作主要排泄蛋白，MEP或CATL）是肽酶C1家族的成员，已被鉴定为与cathepsin H关系最密切的蛋白质。它是一种溶酶体半胱氨酸蛋白酶，可介导胶原蛋白、弹性蛋白和α-1蛋白酶抑制剂的细胞内蛋白质分解代谢。Cathepsin L是由二硫键连接的重链和轻链组成的二聚体，两者均由单一蛋白质前体产生。已发现该基因至少有两种转录变体编码相同的蛋白质。生长因子或肿瘤启动子刺激的转化小鼠成纤维细胞会分泌一种cathepsin L。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
cathepsin L 抗体 (G-11)	sc-390367	200 µg/ml	RMB2377.00
cathepsin L (G-11): m-IgG Fc BP-HRP 套装	sc-526150	200 µg Ab; 10 µg BP	RMB2662.00
cathepsin L (G-11): m-IgG_2a BP-HRP 套装	sc-546516	200 µg Ab; 10 µg BP	RMB2662.00
cathepsin L 抗体 (G-11) AC	sc-390367 AC	500 µg/ml, 25% agarose	RMB3129.00
cathepsin L 抗体 (G-11) HRP	sc-390367 HRP	200 µg/ml	RMB2377.00
cathepsin L 抗体 (G-11) FITC	sc-390367 FITC	200 µg/ml	RMB2482.00
cathepsin L 抗体 (G-11) PE	sc-390367 PE	200 µg/ml	RMB2580.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 488	sc-390367 AF488	200 µg/ml	RMB2685.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 546	sc-390367 AF546	200 µg/ml	RMB2685.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 594	sc-390367 AF594	200 µg/ml	RMB2685.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 647	sc-390367 AF647	200 µg/ml	RMB2685.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 680	sc-390367 AF680	200 µg/ml	RMB2685.00
cathepsin L 抗体 (G-11) Alexa Fluor^® 790	sc-390367 AF790	200 µg/ml	RMB2685.00
cathepsin L (G-11) 中和勝肽	sc-390367 P	100 µg/0.5 ml	RMB511.00

What is the dilution ration for sc-390367 on WB

Asked by: YUAN545

Our protocol recommends a starting dilution of 1:200 and adjusting your concentration from there to achieve desired results. The typical dilution range of our monoclonal antibodies is between 1:100 and 1:1000.

Answered by: Dallas Tech Service

Date published: 2021-04-07

What application is the blocking peptide sc-390367 P appropriate for?

Asked by: Randy McDonald

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 2 out of 5 by neurolover from Did not detect mature forms.Antibody: Cathepsin L (G-11), sc-390367 (Mouse monoclonal, Santa Cruz), 1:900 diluted in 1% Skimmed Milk-TBST. Overnight incubation @ 4°C Samples: SH-SY5Y cell lysates 20µg/lane, 12% Gel Transferred to PVDF membrane; blocked with 5% skimmed milk for 1hr @ RT. was supposed to detect both full length & cleaved Cathepsin L.. But doesn’t detect the low mol wt cleaved fragments. Detects protein at ~36kDa (pro cathepsin)

Date published: 2016-12-04

Rated 5 out of 5 by LF from Strong bands for both pro and mature cathepsinStrong bands for both pro and mature cathepsin L in WB in RAW 264.7, NIH/3T3 and KNRK whole cell lysates. -SCBT QC

Date published: 2014-08-30

cathepsin L 抗体 (G-11) is rated 3.5 out of 5 by 2.

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cathepsin L 抗体 (G-11): sc-390367

cathepsin L 抗体 (G-11) 规格

替代产品名称: CTSL

产品克隆名称: G-11

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2a kappa light chain

氨基酸: 263-291

抗原表位靶标: cathepsin L

抗原表位种属: mouse

产品反应性/检测: cathepsin L

种属反应性/检测: mouse, rat

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 7篇各种科学出版物的引文

優選的 cathepsin L 抗體是 cathepsin L 抗體 (33/2): sc-32320.