Date published: 2025-9-8
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catalase Antibody (H-9) - Western Blotting - Image 375641
过氧化氢酶抗体 (H-9): sc-271803. 3T3-L1 (A) 和 PC-12 (B) 全细胞裂解液以及人骨髓 (C) 和人肾 (D) 组织提取物中过氧化氢酶表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
过氧化氢酶抗体 (H-9): sc-271803. 3T3-L1 (A) 和 PC-12 (B) 全细胞裂解液以及人骨髓 (C) 和人肾 (D) 组织提取物中过氧化氢酶表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

catalase 抗体 (H-9): sc-271803

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说明书
  • catalase 抗体 H-9 是小鼠单克隆 IgG2a κ，catalase抗体, 在122篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的catalase的C-terminus附近的氨基酸471-503之间
  • catalase 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 catalase
  • 抗catalase抗体（H-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取catalase (H-9): sc-271803的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是catalase Antibody (H-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与catalase Antibody (H-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

过氧化氢酶抗体(H-9)是一种IgG2a κ小鼠单克隆过氧化氢酶抗体(也称为CAT抗体)，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源过氧化氢酶蛋白。过氧化氢酶抗体(H-9)既可以是非偶联抗过氧化氢酶抗体形式，也可以是多种偶联形式的抗过氧化氢酶抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。过氧化氢酶是过氧化物酶体特有的标志蛋白，属于过氧化氢酶家族。编码过氧化氢酶蛋白的基因缺陷可引起无过氧化氢酶症，这是一种以红细胞中缺乏过氧化氢酶活性为特征的疾病，并与溃疡性口腔病变相关。过氧化氢酶也是氧化应激和炎症的重要调节因子，并可能促进类风湿性关节炎的发展。过氧化氢酶可以形成同源四聚体，几乎存在于所有有氧呼吸的生物体中，具有保护细胞免受过氧化氢毒性影响的功能。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

catalase 抗体 (H-9) 参考文献:

  1. Pro12Ala PPARgamma2 基因错义突变与瑞典中年男性肥胖和胰岛素抵抗有关。  |  Rosmond, R., et al. 2003. Diabetes Metab Res Rev. 19: 159-63. PMID: 12673785
  2. 过氧化物酶体增殖激活受体-γ2-Pro12Ala 和内皮一氧化氮合酶-4a/b 基因多态性与本质性高血压有关。  |  Rodríguez-Esparragón, FJ., et al. 2003. J Hypertens. 21: 1649-55. PMID: 12923396
  3. 韩国人的过氧化氢酶和 PPARgamma2 基因型与患系统性红斑狼疮的风险。  |  Eny, KM., et al. 2005. Lupus. 14: 351-5. PMID: 15934434
  4. 15d-前列腺素 J2 可激活过氧化物酶体-活化受体-γ，促进过氧化氢酶的表达，并减轻大鼠脑内出血后的炎症, 行为功能障碍和神经元损失。  |  Zhao, X., et al. 2006. J Cereb Blood Flow Metab. 26: 811-20. PMID: 16208315
  5. 过氧物酶体与衰老  |  Terlecky, SR., et al. 2006. Biochim Biophys Acta. 1763: 1749-54. PMID: 17027095
  6. 过氧化氢酶在肺纤维化中的作用。  |  Odajima, N., et al. 2010. Respir Res. 11: 183. PMID: 21190578
  7. 过氧化氢酶和NO CATALASE ACTIVITY1促进拟南芥细胞凋亡。  |  Hackenberg, T., et al. 2013. Plant Cell. 25: 4616-26. PMID: 24285797
  8. 氧化应激和过氧化氢酶基因。  |  Ershova, OA., et al. 2016. Bull Exp Biol Med. 161: 400-3. PMID: 27496033
  9. 用于固定过氧化氢酶的氧化功能化冷冻凝胶盘  |  Inanan, T., et al. 2018. Int J Biol Macromol. 114: 812-820. PMID: 29626600
  10. 有效提高过氧化氢酶性能的环保方法。  |  Leonida, MD., et al. 2024. Int J Biol Macromol. 280: 135597. PMID: 39278428
  11. 假性婴儿雷弗森氏病：过氧化物酶缺陷型过氧化物酶体颗粒部分缺乏质原合成和脂肪酸氧化。  |  Aubourg, P., et al. 1993. Pediatr Res. 34: 270-6. PMID: 7510868
  12. 韩国人的过氧化氢酶和 PPARgamma2 基因型与类风湿性关节炎的风险。  |  El-Sohemy, A., et al. 2006. Rheumatol Int. 26: 388-92. PMID: 15988600

订购信息

产品名称产品编号规格价格数量收藏夹

catalase 抗体 (H-9)

sc-271803
200 µg/ml
RMB2377.00

catalase (H-9): m-IgG Fc BP-HRP 套装

sc-529264
200 µg Ab; 10 µg BP
RMB2662.00

catalase (H-9): m-IgGκ BP-HRP 套装

sc-522066
200 µg Ab, 40 µg BP
RMB2662.00

catalase (H-9): m-IgG2a BP-HRP 套装

sc-547287
200 µg Ab; 10 µg BP
RMB2662.00

catalase 抗体 (H-9) AC

sc-271803 AC
500 µg/ml, 25% agarose
RMB3129.00

catalase 抗体 (H-9) HRP

sc-271803 HRP
200 µg/ml
RMB2377.00

catalase 抗体 (H-9) FITC

sc-271803 FITC
200 µg/ml
RMB2482.00

catalase 抗体 (H-9) PE

sc-271803 PE
200 µg/ml
RMB2580.00

catalase 抗体 (H-9) Alexa Fluor® 488

sc-271803 AF488
200 µg/ml
RMB2685.00

catalase 抗体 (H-9) Alexa Fluor® 546

sc-271803 AF546
200 µg/ml
RMB2685.00

catalase 抗体 (H-9) Alexa Fluor® 594

sc-271803 AF594
200 µg/ml
RMB2685.00

catalase 抗体 (H-9) Alexa Fluor® 647

sc-271803 AF647
200 µg/ml
RMB2685.00

catalase 抗体 (H-9) Alexa Fluor® 680

sc-271803 AF680
200 µg/ml
RMB2685.00

catalase 抗体 (H-9) Alexa Fluor® 790

sc-271803 AF790
200 µg/ml
RMB2685.00

catalase (H-9) 中和勝肽

sc-271803 P
100 µg/0.5 ml
RMB511.00

I am looking at this catalase antibody. Is it known whether it cross reacts with the corresponding Drosophila protein? I would need to use it for immunofluorescence.

Asked by: Michael
Thank you for your question. We have not validated the antibody for Drosophila. The antibody is recommended for mouse, rat and human samples.
Answered by: BlakeJ
Date published: 2024-11-22

What is the difference between sc-271803 and sc-271803 P?

Asked by: jenni
Thank you for your question. sc-271803 P is our blocking peptide, meant as a negative control for use with the catalase (H-9): sc-271803 monoclonal antibody.
Answered by: Technical Support 4
Date published: 2018-02-24

What secondary is best to use with catalase (H-9): sc-271803?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with catalase (H-9):sc-271803, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 4 out of 5 by from Anti-catalsae antibodygood antibody that we used for flow cytometry of mice keratinocytes
Date published: 2022-07-27
Rated 4 out of 5 by from It works well in WB!I used it with a 1:100 dilution in BSA. It works well
Date published: 2020-10-13
Rated 5 out of 5 by from Great antibodyIt works well at first try in Western blot using 1:1000 dilution and 1:10 000 secondary antibody. 20 ug mouse brain lysate is tested.
Date published: 2020-07-02
Rated 4 out of 5 by from ottimo funzionamentoHo richiesto questo campione in omaggio insieme ad un ordine. L'ho preparato 1:100 in pbst 1% di marvel. Ottima risoluzione!
Date published: 2019-07-26
Rated 4 out of 5 by from Very good antibodyThis antibody has worked very well in my hands. I use a dilution of 1:1000. I am able to reuse the antibody as many as three times.
Date published: 2019-05-07
Rated 4 out of 5 by from Buon funzionamentol'anticorpo funziona bene in western blot su cellule umane di carcinoma pancreatico
Date published: 2018-12-05
Rated 5 out of 5 by from Works in IFThis antibody works well in my immunostaining experiment with mouse samples.
Date published: 2018-06-05
Rated 5 out of 5 by from WBWe used Catalase (H-9) antibody for WB in Mouse lung primary fibroblast, and it worked fine. Just remember use 1/250 to get good signal. We used 1/500 and it was a bit weak. We used anti-mouse IgG.
Date published: 2018-03-28
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catalase 抗体 (H-9) is rated 4.6 out of 5 by 25.
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