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CASPR2 抗体 (H-10): sc-398454

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说明书
  • CASPR2 抗体 H-10 是小鼠单克隆 IgG2b κ,CASPR2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 针对CASPR2的human起源的N-terminal细胞外域内的氨基酸461-560
  • CASPR2 抗体 (H-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CASPR2
  • 抗CASPR2抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CASPR2 (H-10): sc-398454免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是CASPR2 Antibody (H-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CASPR2 Antibody (H-10) 打包提供(请参阅下面的订购信息)。

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関連項目

CASPR2抗体(H-10)是一种IgG2b κ小鼠单克隆CASPR2抗体(也被称为CASPR2抗体),能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测来自小鼠、大鼠和人类的CASPR2蛋白。CASPR2抗体(H-10)既有非偶联形式的抗CASPR2抗体,也有多种偶联形式的抗CASPR2抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。CASPR(接触素相关蛋白,也被称为Paranodin)是神经连接蛋白超家族的一种跨膜糖蛋白,在髓鞘轴突区域高度富集。在成年神经系统中,髓鞘神经的轴突具有特殊的亚细胞结构,这些结构对于有效和快速的动作电位传播至关重要。CASPR和密切相关的分子CASPR2(果蝇Neurexin IV (Nrx-IV)的哺乳动物同源物)在髓鞘轴突中标定出不同的亚域。CASPR存在于旁结节点,而CASPR2则精确地与共定位于近旁结区域的Shaker样K+通道共存。CASPR2特异性地与Kv1.1、Kv1.2及其Kvβ2亚基相关联。CASPR家族成员可能在轴突局部分化为不同的功能亚域中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CASPR2 抗体 (H-10) 参考文献:

  1. Caspr2是神经毒素超家族的一个新成员,定位于有髓鞘轴突的并轴,并与K+通道相关联。  |  Poliak, S., et al. 1999. Neuron. 24: 1037-47. PMID: 10624965
  2. 髓鞘施旺细胞决定了 Kv1.1, Kv1.2, Kvbeta2 和 Caspr 的节间定位。  |  Arroyo, EJ., et al. 1999. J Neurocytol. 28: 333-47. PMID: 10739575
  3. 糖基磷脂酰肌醇锚定粘附分子 F3/contactin 是副胰岛素/contactin 相关蛋白(caspr)表面转运所必需的。  |  Faivre-Sarrailh, C., et al. 2000. J Cell Biol. 149: 491-502. PMID: 10769038
  4. 轴突与胶质细胞的相互作用:构建智能神经纤维  |  Waxman, SG. 1997. Curr Biol. 7: R406-10. PMID: 9210363
  5. 轴突膜蛋白Caspr是神经肽IV的同源物,是髓鞘化过程中组装的隔板状副结节的一个组成部分。  |  Einheber, S., et al. 1997. J Cell Biol. 139: 1495-506. PMID: 9396755
  6. 神经毒素:三个基因和 1001 种产品。  |  Missler, M. and Südhof, TC. 1998. Trends Genet. 14: 20-6. PMID: 9448462
  7. 神经肽 IV, caspr 和 paranodin--神经肽家族的新成员:轴突和神经胶质的相遇。  |  Bellen, HJ., et al. 1998. Trends Neurosci. 21: 444-9. PMID: 9786343

订购信息

产品名称产品编号规格价格数量收藏夹

CASPR2 抗体 (H-10)

sc-398454
200 µg/ml
RMB2377.00

CASPR2 (H-10): m-IgG Fc BP-HRP 套装

sc-530804
200 µg Ab; 10 µg BP
RMB2662.00

CASPR2 (H-10): m-IgGκ BP-HRP 套装

sc-524348
200 µg Ab, 40 µg BP
RMB2662.00

CASPR2 (H-10): m-IgG2b BP-HRP 套装

sc-550230
200 µg Ab; 10 µg BP
RMB2662.00

CASPR2 抗体 (H-10) AC

sc-398454 AC
500 µg/ml, 25% agarose
RMB3129.00

CASPR2 抗体 (H-10) HRP

sc-398454 HRP
200 µg/ml
RMB2377.00

CASPR2 抗体 (H-10) FITC

sc-398454 FITC
200 µg/ml
RMB2482.00

CASPR2 抗体 (H-10) PE

sc-398454 PE
200 µg/ml
RMB2580.00

CASPR2 抗体 (H-10) Alexa Fluor® 488

sc-398454 AF488
200 µg/ml
RMB2685.00

CASPR2 抗体 (H-10) Alexa Fluor® 546

sc-398454 AF546
200 µg/ml
RMB2685.00

CASPR2 抗体 (H-10) Alexa Fluor® 594

sc-398454 AF594
200 µg/ml
RMB2685.00

CASPR2 抗体 (H-10) Alexa Fluor® 647

sc-398454 AF647
200 µg/ml
RMB2685.00

CASPR2 抗体 (H-10) Alexa Fluor® 680

sc-398454 AF680
200 µg/ml
RMB2685.00

CASPR2 抗体 (H-10) Alexa Fluor® 790

sc-398454 AF790
200 µg/ml
RMB2685.00

Can we use this antibody for IHC (DAB) in fixed rat brain? Thank you

Asked by: Cleusa
We have not yet tested this antibody in formalin-fixed paraffin-embedded tissue, and so IHC(P) is not a recommended application. This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-04-13

What is the recommended starting dilution for Western blot for CASPR2 (H-10): sc-398454 monoclonal antibody?

Asked by: Cweed
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-398454.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great Western Blot data of CASPR2 expressionGreat Western Blot data of CASPR2 expression in human lateral ventricle and human hypothalamus tissue extracts. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Great Western Blot data of CASPR2 expressionGreat Western Blot data of CASPR2 expression in human hippocampus tissue extract. -SCBT QC
Date published: 2014-03-13
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CASPR2 抗体 (H-10) is rated 5.0 out of 5 by 2.
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