Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
caspase-9 p10 Antibody (F-7) - Western Blotting - Image 352784
caspase-9 (F-7): sc-17784. 对 Jurkat (A), K-562 (B), HCT-116 (C), Saos-2 (D), COLO 205 (E) 和 HEK293 (F) 全细胞裂解液中 procaspase-9 的表达进行 Western 印迹分析.
caspase-9 (F-7): sc-17784. 对 Jurkat (A), K-562 (B), HCT-116 (C), Saos-2 (D), COLO 205 (E) 和 HEK293 (F) 全细胞裂解液中 procaspase-9 的表达进行 Western 印迹分析.

caspase-9 p10 抗体 (F-7): sc-17784

4.9(7)
寫評論提問

说明书
  • caspase-9 p10抗体(F-7)是小鼠单克隆IgG2a κ, 在104篇文献中引用，规格为200 µg/ml
  • 针对human的caspase-9 p10的C-terminus转录激活域内的315-397氨基酸的
  • caspase-9 p10 抗体 (F-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 precursor and p10 subunit of caspase-9
  • 抗caspase-9 p10抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-9 p10 (F-7): sc-17784的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 caspase-9 p10 抗体 (F-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-9 p10 抗体 (F-7) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Caspase 抗体 包括 caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5和caspase-6

caspase-9 p10抗体（F-7）是一种鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫 酶联免疫吸附试验（ELISA）。抗半胱天冬酶-9 p10 抗体（F-7）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物。Caspase-9是细胞凋亡通路的关键组成部分，作为启动酶，它响应促凋亡信号，激活下游效应酶caspase-3和caspase-7。这种激活对于执行程序性细胞死亡至关重要，而程序性细胞死亡对于维持细胞稳态和消除受损或有害细胞必不可少。对半胱天冬酶-9活性的适当调节至关重要，因为失调会导致包括癌症在内的多种疾病，而癌细胞逃避凋亡会导致细胞无序增殖。半胱天冬酶-9与包括Apaf-1在内的多种蛋白相互作用，Apaf-1形成凋亡复合物，促进半胱天冬酶-9激活，以响应线粒体释放的细胞色素c。了解这些相互作用以及涉及半胱天冬酶-9的调节机制对于制定旨在调节疾病中凋亡的治疗策略非常重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-9 p10 抗体 (F-7) 参考文献:

  1. caspase-3 和 XIAP 对 Apaf-1/caspase-9 细胞凋亡小体的调控  |  Zou, H., et al. 2003. J Biol Chem. 278: 8091-8. PMID: 12506111
  2. 半胱氨酸蛋白酶-9：结构、机制和临床应用。  |  Li, P., et al. 2017. Oncotarget. 8: 23996-24008. PMID: 28177918
  3. Caspase-9：一种在人类疾病中具有多种细胞表达的多模式治疗靶标。  |  Avrutsky, MI. and Troy, CM. 2021. Front Pharmacol. 12: 701301. PMID: 34305609
  4. Caspase-9抑制剂触发Hsp90介导的化疗肿瘤固有先天感应并增强抗肿瘤免疫。  |  Li, J., et al. 2023. J Immunother Cancer. 11: PMID: 38056894
  5. DNA 链断裂诱导的聚（ADP-核糖）聚合酶翻译后修饰。  |  Lindahl, T., et al. 1995. Trends Biochem Sci. 20: 405-11. PMID: 8533153
  6. ICE-LAP3是草履虫细胞死亡蛋白Ced-3的哺乳动物同源物，在Fas和肿瘤坏死因子诱导的细胞凋亡过程中被激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
  7. Apopain/CPP32 可裂解细胞修复所必需的蛋白质：细胞凋亡的基本原理。  |  Casciola-Rosen, L., et al. 1996. J Exp Med. 183: 1957-64. PMID: 8642305
  8. ICE-LAP6 是 ICE/Ced-3 基因家族的一个新成员，它被细胞毒性 T 细胞蛋白酶颗粒酶 B 激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
  9. Mch2 alpha 而非 CPP32 对层粘蛋白 A 的裂解：多种白细胞介素 1 β-转化酶相关蛋白酶在细胞凋亡过程中具有不同的底物识别特性。  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
  10. Mch4体外激活CPP32和Mch3，Mch4是一种新型人类凋亡半胱氨酸蛋白酶，含有两个类似FADD的结构域。  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  11. 多聚（ADP-核糖）聚合酶在与分化相关的 DNA 复制过程中的表达表明，它是多蛋白 DNA 复制复合体的一个组成部分。  |  Simbulan-Rosenthal, CM., et al. 1996. Biochemistry. 35: 11622-33. PMID: 8794742
  12. Caspases：细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-9 p10 抗体 (F-7)

sc-17784
200 µg/ml
RMB2377.00

caspase-9 p10 (F-7): m-IgG Fc BP-HRP 套装

sc-528311
200 µg Ab; 10 µg BP
RMB2662.00

caspase-9 p10 (F-7): m-IgGκ BP-HRP 套装

sc-520655
200 µg Ab, 40 µg BP
RMB2662.00

caspase-9 p10 (F-7): m-IgG2a BP-HRP 套装

sc-547069
200 µg Ab; 10 µg BP
RMB2662.00

caspase-9 p10 抗体 (F-7) AC

sc-17784 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-9 p10 抗体 (F-7) HRP

sc-17784 HRP
200 µg/ml
RMB2377.00

caspase-9 p10 抗体 (F-7) FITC

sc-17784 FITC
200 µg/ml
RMB2482.00

caspase-9 p10 抗体 (F-7) PE

sc-17784 PE
200 µg/ml
RMB2580.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 488

sc-17784 AF488
200 µg/ml
RMB2685.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 546

sc-17784 AF546
200 µg/ml
RMB2685.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 594

sc-17784 AF594
200 µg/ml
RMB2685.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 647

sc-17784 AF647
200 µg/ml
RMB2685.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 680

sc-17784 AF680
200 µg/ml
RMB2685.00

caspase-9 p10 抗体 (F-7) Alexa Fluor® 790

sc-17784 AF790
200 µg/ml
RMB2685.00

Is this antibody human specific or also works on murine cell lines/tissue?

Asked by: FionaJ
Thank you for your question. sc-17784 is recommended for detection of precursor and p10 subunit of caspase-9 of human origin
Answered by: Tech Support Europe
Date published: 2021-08-10

Hello my name is Aidan. I am doing a research project at UT Southwester research center. I am looking into using a western blot and caspase-9 to detect the amount of P10 in the cell but do not know what lysis buffer to use. do you have any recommendation?

Asked by: Aidan
Thank you for your question. We do not yet have a specific protocol recommended for detection of the p10 subunit of caspase-9 with this antibody. We use our standard Western blotting protocol for testing this antibody and observe strong and specific signal for the precursor protein. We use our RIPA Lysis buffer. We would recommend checking some of the product citations this antibody for Western blotting, to see if there is any specific recommendation for inducing and detecting the p10 subunit by Western blot.
Answered by: Technical Support
Date published: 2020-04-03

Can you recommend a positive control to use with caspase-9 p10 (F-7): sc-17784 monoclonal antibody for Western blot?

Asked by: jenni
We recommend Jurkat (sc-2204), K-562 (sc-2203), HCT-116 (sc-364175), Saos-2 (sc-2235), COLO 205 (sc-364177) or HEK 293 (sc-45136) whole cell lysates to use as positive controls as we have great Western blot data showing specific detection of caspase-9 p10 in these cell lines.
Answered by: Technical Support 15
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with caspase-9 p10 (F-7): sc-17784?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_17784, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from GOODNice good antibody... Clear strong signal . .... .
Date published: 2017-12-21
Rated 5 out of 5 by from Good antibody for WBUsed for detecting basal Caspase-9 level in 293T cells, dilute 1:1000 in western blot, clear full size band.
Date published: 2017-12-05
Rated 5 out of 5 by from Wow !! Superb productJust a great antibody for multiple applications; hard to compare !!
Date published: 2017-01-31
Rated 5 out of 5 by from Great caspase-9 antibodyGreat antibody with specificity to caspase-9 shown in siRNA knockdown studies. Great antibody for caspase-9 precursor and p10 detection.
Date published: 2017-01-27
Rated 5 out of 5 by from Used in Western with SGCUsed in Western with SGC-7901, BGC-823 cells. - SCBT Publication Review
Date published: 2013-12-02
Rated 5 out of 5 by from Works with MDAWorks with MDA-Panc-3, Panc-1, U2OS and SOAS2 cells in Western. - SCBT Publication Review
Date published: 2013-09-05
Rated 5 out of 5 by from Good results with RPMIGood results with RPMI-8226, OPM-2 , U-266 cells and T-cell leukaemia KE-37 cells. - SCBT Publication Review
Date published: 2008-01-15
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_17784, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
caspase-9 p10 抗体 (F-7) is rated 4.9 out of 5 by 7.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_17784, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。