Date published: 2025-9-6
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caspase-8 Antibody (8CSP03) - Western Blotting - Image 388001
caspase-8 抗体 (8CSP03) HRP: sc-56070 HRP. 对 HeLa (A), Jurkat (B), CCRF-CEM (C), MOLT-4 (D), HL-60 (E) 和 SW480 (F) 全细胞裂解液中 procaspase-8 的表达进行直接 Western 印迹分析.
caspase-8 抗体 (8CSP03) HRP: sc-56070 HRP. 对 HeLa (A), Jurkat (B), CCRF-CEM (C), MOLT-4 (D), HL-60 (E) 和 SW480 (F) 全细胞裂解液中 procaspase-8 的表达进行直接 Western 印迹分析.

caspase-8 抗体 (8CSP03): sc-56070

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寫評論提問

说明书
  • caspase-8 抗体 8CSP03 是小鼠单克隆 IgG1 κ，caspase-8抗体, 在87篇文献中引用，规格为100 µg/ml
  • 重组全长 human caspase-8 蛋白
  • caspase-8 抗体 (8CSP03) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 caspase-8
  • 抗caspase-8抗体（8CSP03）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-8 (8CSP03): sc-56070的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 caspase-8 抗体 (8CSP03) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-8 抗体 (8CSP03) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Caspase 抗体 包括 caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5和caspase-6

caspase-8抗体（8CSP03）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ 酶联免疫吸附试验）。抗半胱天冬酶-8抗体（8CSP03）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。半胱天冬酶-8在细胞凋亡信号传导通路中起着至关重要的作用，它作为起始半胱天冬酶，通过切割下游效应半胱天冬酶的非活性前体来激活它们。这一过程对于调节程序性细胞死亡至关重要，特别是通过肿瘤坏死因子受体超家族成员1A（TNFRSF1A）和Fas（TNFRSF6）对死亡受体信号的响应。半胱氨酸蛋白酶-8主要位于细胞质中，与适配分子FADD结合后进入死亡诱导信号复合物（DISC），并在那里激活。半胱氨酸蛋白酶-8的活性形式是由p18和p10亚基组成的异源二聚体，然后从DISC中释放出来，以传播凋亡信号。Caspase-8广泛表达，在外周血白细胞、胸腺、肝脏和脾脏中的水平最高。CASP8基因的突变或缺陷可导致caspase-8缺乏症（CASP8D），该疾病与脾肿大和外周血白细胞凋亡受损有关，并可能因T淋巴细胞、自然杀伤细胞和B淋巴细胞的激活受损而导致免疫缺陷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-8 抗体 (8CSP03) 参考文献:

  1. 在没有 CD95 受体/配体相互作用的情况下，抗癌药物会诱导 caspase-8/FLICE 激活和细胞凋亡。  |  Wesselborg, S., et al. 1999. Blood. 93: 3053-63. PMID: 10216102
  2. FADD和caspase-8信号参与了脱落诱导的细胞凋亡。  |  Rytömaa, M., et al. 1999. Curr Biol. 9: 1043-6. PMID: 10508619
  3. 细胞凋亡的关键激活因子--人类 Caspase-8 的原子分辨率结构。  |  Watt, W., et al. 1999. Structure. 7: 1135-43. PMID: 10508785
  4. Pro-caspase-8 主要定域于线粒体，受到凋亡刺激后释放到细胞质中。  |  Qin, ZH., et al. 2001. J Biol Chem. 276: 8079-86. PMID: 11102441
  5. 独立于 Fas（CD95/APO-1）信号的 Caspase 8 激活可能是细胞毒性药物或伽马射线杀死 B 慢性淋巴细胞白血病细胞的介导因素。  |  Jones, DT., et al. 2001. Blood. 98: 2800-7. PMID: 11675354
  6. p38-MAPK通过磷酸化人中性粒细胞中的caspase-8和caspase-3发出生存信号。  |  Alvarado-Kristensson, M., et al. 2004. J Exp Med. 199: 449-58. PMID: 14970175
  7. caspase-8在卵巢癌肿瘤微环境中的作用。  |  Newton, K., et al. 2019. Nature. 575: 679-682. PMID: 31723262
  8. 法斯死亡因素  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  9. FasL/TNF 死亡信号的竞争者。  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
  10. Mch4体外激活CPP32和Mch3，Mch4是一种新型人类凋亡半胱氨酸蛋白酶，含有两个类似FADD的结构域。  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  11. FLICE 通过与 CD95 死亡诱导信号复合体（DISC）结合而激活。  |  Medema, JP., et al. 1997. EMBO J. 16: 2794-804. PMID: 9184224
  12. Bcl-xL 在 Caspase-8 下游发挥作用，抑制 Fas 和肿瘤坏死因子受体 1 诱导的 MCF7 乳腺癌细胞凋亡。  |  Srinivasan, A., et al. 1998. J Biol Chem. 273: 4523-9. PMID: 9468507

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-8 抗体 (8CSP03)

sc-56070
100 µg/ml
RMB2377.00

caspase-8 (8CSP03): m-IgG Fc BP-HRP 套装

sc-526873
100 µg Ab; 10 µg BP
RMB2662.00

caspase-8 (8CSP03): m-IgG1 BP-HRP 套装

sc-532246
100 µg Ab; 20 µg BP
RMB2662.00

caspase-8 抗体 (8CSP03) AC

sc-56070 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-8 抗体 (8CSP03) HRP

sc-56070 HRP
200 µg/ml
RMB2377.00

caspase-8 抗体 (8CSP03) FITC

sc-56070 FITC
200 µg/ml
RMB2482.00

caspase-8 抗体 (8CSP03) PE

sc-56070 PE
200 µg/ml
RMB2580.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 488

sc-56070 AF488
200 µg/ml
RMB2685.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 546

sc-56070 AF546
200 µg/ml
RMB2685.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 594

sc-56070 AF594
200 µg/ml
RMB2685.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 647

sc-56070 AF647
200 µg/ml
RMB2685.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 680

sc-56070 AF680
200 µg/ml
RMB2685.00

caspase-8 抗体 (8CSP03) Alexa Fluor® 790

sc-56070 AF790
200 µg/ml
RMB2685.00

Do you have any anti-caspase 8 antibody likely to cross-react with the drosophila homolog dredd Q8IRY7 in your antibody collection? thank you

Asked by: Veronique
Thank you for your question. Most of our antibodies are only tested in samples from mouse, rat, and human. We unfortunately do not have information on reactivity of our caspase 8 antibodies with the drosophila homolog.
Answered by: Technical Support Europe
Date published: 2020-10-08

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-12

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-56070 PE), fluorescein (sc-56070 FITC), Alexa Fluor® 488 (sc-56070 AF488) or Alexa Fluor® 647 (sc-56070 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-12
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Rated 4 out of 5 by from Acceptable for detection non-cleaved Caspase8 (WB)Antibody specifically recognizes non-cleaved Caspase 8 , however detection in lysates of HCT116 cells was rather week. I recommend to use fresh lysates and load more ug of protein on gels.
Date published: 2022-11-18
Rated 4 out of 5 by from Excellent.This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 5 out of 5 by from GOODThe antibody work perfect with NCI-H520 LUNG cell line with a dilution of 1:200
Date published: 2017-09-03
Rated 4 out of 5 by from GreatWestern blot (dilution 1:100), the results are mazing
Date published: 2017-08-19
Rated 5 out of 5 by from Works great at ratio 1:500Works great at ratio 1:500; Blocking: 5% milk in TBST; First Ab: 1:500 5% milk in TBST; Second Ab: 1:5000 5% milk in TBST;
Date published: 2017-08-01
Rated 1 out of 5 by from recommend this antibodyI had detected this antbody,I loaded 20ug sample, The antibody was diluted 1:200 for WB analyisis，but it did not work for turbot intestine tissue .
Date published: 2017-07-18
Rated 5 out of 5 by from Excellent results in WBI used this antibody for several times. It gives me excellent strong results every time. I will always use it.
Date published: 2017-07-12
Rated 5 out of 5 by from GreatSpecific bands for western blotting, I diluted with 1:500. It can be detected in mkn-45 cell.
Date published: 2017-06-14
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caspase-8 抗体 (8CSP03) is rated 4.4 out of 5 by 22.
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