Date published: 2025-9-6

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caspase-8 抗体 (1.1.40): sc-81656

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说明书
  • caspase-8抗体(1.1.40)是小鼠单克隆IgG1 κ, 在37篇文献中引用,规格为200 µg/ml
  • 与全长重组蛋白caspase-8(human起源)杂交
  • 抗-caspase-8 抗体 (1.1.40) 推荐用于 WB 和 IP,检测mouse, rat 和human 来源的 caspase-8
  • 抗caspase-8抗体(1.1.40)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-8 (1.1.40): sc-81656免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是caspase-8 Antibody (1.1.40) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与caspase-8 Antibody (1.1.40) 打包提供(请参阅下面的订购信息)。

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関連項目

caspase-8抗体(1.1.40)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)检测小鼠、大鼠和人类样本中的caspase-8。抗-caspase-8抗体(1.1.40)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。半胱天冬酶-8在细胞凋亡途径中起着至关重要的作用,它作为启动半胱天冬酶,通过切割其非活性前体来激活下游效应半胱天冬酶。这种激活对于程序性细胞死亡过程至关重要,特别是响应肿瘤坏死因子受体超家族成员(如TNFRSF1A和TNFRSF6/FAS)的信号时。Caspase-8主要位于细胞质中,与适配子分子FADD形成复合物,从而形成死亡诱导信号复合物(DISC)。该复合物对于激活caspase-8至关重要,随后会分解并激活其他凋亡蛋白酶,从而传播凋亡信号。Caspase-8广泛表达,在外周血白细胞、胸腺、肝脏和脾脏中的含量最高。CASP8基因编码Caspase-8,其缺陷可导致Caspase-8缺乏症(CASP8D),其症状为脾肿大和外周血白细胞凋亡受损,由于T淋巴细胞、自然杀伤细胞和B淋巴细胞的激活受损,可能导致免疫缺陷。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-8 抗体 (1.1.40)

sc-81656
200 µg/ml
RMB2377.00

caspase-8 (1.1.40): m-IgG Fc BP-HRP 套装

sc-528710
200 µg Ab; 10 µg BP
RMB2662.00

caspase-8 (1.1.40): m-IgGκ BP-HRP 套装

sc-521178
200 µg Ab, 40 µg BP
RMB2662.00

caspase-8 (1.1.40): m-IgG1 BP-HRP 套装

sc-543081
200 µg Ab; 20 µg BP
RMB2662.00

caspase-8 抗体 (1.1.40) AC

sc-81656 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-8 抗体 (1.1.40) HRP

sc-81656 HRP
200 µg/ml
RMB2377.00

caspase-8 抗体 (1.1.40) FITC

sc-81656 FITC
200 µg/ml
RMB2482.00

caspase-8 抗体 (1.1.40) PE

sc-81656 PE
200 µg/ml
RMB2580.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 488

sc-81656 AF488
200 µg/ml
RMB2685.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 546

sc-81656 AF546
200 µg/ml
RMB2685.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 594

sc-81656 AF594
200 µg/ml
RMB2685.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 647

sc-81656 AF647
200 µg/ml
RMB2685.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 680

sc-81656 AF680
200 µg/ml
RMB2685.00

caspase-8 抗体 (1.1.40) Alexa Fluor® 790

sc-81656 AF790
200 µg/ml
RMB2685.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-20
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Rated 4 out of 5 by from 抗体很不错!我在一个月前购买了此产品,用于细胞蛋白WB实验,能很好的跑出目的调到,且可以获得相应的剪切体条带,很满意!
Date published: 2022-09-09
Rated 3 out of 5 by from good antibodyThis antibody was used in WB analysis in mouse tumor tissues, diluted 250 times in TBST 1x and milk 2%. I got bands in every sample, but faint.
Date published: 2018-05-11
Rated 2 out of 5 by from western blotting-okay for precursor band!This antibody was used for western blotting (1:200 dilution) of AC16 cell lysates. The precursor band was visible at the expected molecular weight but the cleaved caspase-8 band, though visible, was not at the predicted molecular weight.
Date published: 2018-04-27
Rated 5 out of 5 by from Great westernHighly specific western; strong signal with low background
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of caspase-8 precursor expression in Jurkat whole cell lysate.
Date published: 2017-01-12
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal strength with untreated and treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Clean blot with single strong band in JurkatClean blot with single strong band in Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-06
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caspase-8抗体 (1.1.40) is rated 4.0 out of 5 by 7.
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