Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

caspase-3 p17 抗体 (D-12): sc-373730

4.3(30)
寫評論提問

说明书
  • caspase-3 p17抗体(D-12)是小鼠单克隆IgG2b κ, 在45篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的caspase-3 p17的内部区域内的氨基酸50-86之间
  • 抗-caspase-3 p17 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 caspase-3 p17 and full length procaspase-3
  • 抗caspase-3 p17抗体(D-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-3 p17 (D-12): sc-373730免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 caspase-3 p17 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-3 p17 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

caspase-3 p17 抗体(D-12)是一种 IgG2b κ 小鼠单克隆 caspase-3 p17 抗体,可通过 WB、IP、IF、IHC(P) 和 ELISA 检测到小鼠、大鼠和人类来源的 caspase-3 p17 和全长前体 caspase-3。caspase-3 p17 抗体(D-12)既可以是非偶联抗 caspase-3 p17 抗体的形式,也可以是多种偶联形式的抗 caspase-3 p17 抗体,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Caspase-3,也称为 apopain、SCA-1、Yama 和 CPP32,是一种天冬氨酸特异性的半胱氨酸蛋白酶,属于 caspase 的 ICE 亚家族。Caspase-3 在细胞中作为一种无活性的前体表达,在凋亡过程中,成熟的 caspase-3 的 p17 和 p11 亚基从前体中蛋白水解产生。Caspase-3 前体首先在 Asp 175-Ser 176 处被裂解,产生 p11 亚基和 p20 肽。随后,p20 肽在 Asp 28-Ser 29 处被裂解,生成成熟的 p17 亚基。活性 caspase-3 酶是由两个 p17 和两个 p11 亚基组成的异二聚体。在凋亡开始时,caspase-3 在 Asp 216-Gly 217 键处蛋白水解切割 PARP。在执行凋亡级联反应过程中,活化的 caspase-3 以不同于其正常固醇依赖性激活的蛋白水解反应从 ER 膜上释放 SREBP。Caspase-3 在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间切割并激活 SREBPs。此外,Caspase-3 还切割并激活 caspase-6、-7 和 -9。人类 caspase-3 基因编码一种细胞质蛋白,在肺、脾、心、肝、肾和免疫系统中高度表达。该抗体是研究细胞凋亡、疾病发生机制以及药物筛选等领域的重要工具。通过对 caspase-3 p17 的检测,可以深入了解其在细胞凋亡等生物过程中的作用,为相关疾病的治疗和药物研发提供重要依据。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 p17 抗体 (D-12)

sc-373730
200 µg/ml
$316.00

caspase-3 p17 (D-12): m-IgGκ BP-HRP 套装

sc-522475
200 µg Ab, 40 µg BP
$354.00

caspase-3 p17 (D-12): m-IgG2b BP-HRP 套装

sc-549037
200 µg Ab; 10 µg BP
$354.00

caspase-3 p17 抗体 (D-12) AC

sc-373730 AC
500 µg/ml, 25% agarose
$416.00

caspase-3 p17 抗体 (D-12) HRP

sc-373730 HRP
200 µg/ml
$316.00

caspase-3 p17 抗体 (D-12) FITC

sc-373730 FITC
200 µg/ml
$330.00

caspase-3 p17 抗体 (D-12) PE

sc-373730 PE
200 µg/ml
$343.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 488

sc-373730 AF488
200 µg/ml
$357.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 546

sc-373730 AF546
200 µg/ml
$357.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 594

sc-373730 AF594
200 µg/ml
$357.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 647

sc-373730 AF647
200 µg/ml
$357.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 680

sc-373730 AF680
200 µg/ml
$357.00

caspase-3 p17 抗体 (D-12) Alexa Fluor® 790

sc-373730 AF790
200 µg/ml
$357.00

caspase-3 p17 (D-12) 中和勝肽

sc-373730 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373730 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_373730, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 122ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Quite good antibody1:200 diluted for wb of rat protein ,the band is at 15kd
Date published: 2019-06-17
Rated 3 out of 5 by from It was greatIt was great but doesn't work well after a new batch.
Date published: 2018-06-29
Rated 5 out of 5 by from caspase-3 p17Western blot of mouse primary cerebellar neuron conjugated with caspase-3 p17 (1:1000). Protein concentration 30 µg per lane. Strong expression with low background.
Date published: 2018-03-09
Rated 5 out of 5 by from Good productionwe used TG mice Hip as sample,and the result as we expected,
Date published: 2018-03-01
Rated 4 out of 5 by from Weak signal at 17kDa on Western Blot.Detects doublet around 36kDa (uncleaved form) a weakly detects 17kDa band. Needs further optimizing.
Date published: 2018-01-31
Rated 4 out of 5 by from good antibodyI have used the caspase-3 p17 antibody, the total band is so clear and the p17 band is less than the total band.
Date published: 2017-10-06
Rated 3 out of 5 by from weak bands can be seenyou can see weak bands with the antibody. The Ph value of 1*TBS buffer is 7.45. I haven't try a buffer with lower Ph value.
Date published: 2017-09-30
Rated 5 out of 5 by from Great for early apoptosis detectionGot this Ab as a sample, and tested on HEK cells, seems to work great.
Date published: 2017-09-25
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_30
  • loc_zh_CN, sid_373730, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
caspase-3 p17 抗体 (D-12) is rated 4.3 out of 5 by 30.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_30
  • loc_zh_CN, sid_373730, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 102ms
  • REVIEWS, PRODUCT