Date published: 2025-9-7
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caspase-3 p17 Antibody (B-4) - Western Blotting - Image 377405
Caspase-3 B-4): sc-271028. Jurkat (A), MOLT-4 (B), BJAB (C) 和 SUP-T1 (D) 全细胞裂解液中 procaspase-3 表达的 Western 印迹分析.
Caspase-3 B-4): sc-271028. Jurkat (A), MOLT-4 (B), BJAB (C) 和 SUP-T1 (D) 全细胞裂解液中 procaspase-3 表达的 Western 印迹分析.

caspase-3 p17 抗体 (B-4): sc-271028

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说明书
  • caspase-3 p17抗体(B-4)是小鼠单克隆IgG2a κ, 在72篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列56-104位于human物种的caspase-3 p17的N-terminus附近
  • caspase-3 p17 抗体 (B-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 p17 subunit and full length precursor of caspase-3
  • 抗caspase-3 p17抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-3 p17 (B-4): sc-271028的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 caspase-3 p17 抗体 (B-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-3 p17 抗体 (B-4) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Caspase抗体，包括caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5, caspase-6, caspase-7, caspase-8和caspase-9

caspase-3 p17抗体（B-4）是一种IgG2a κ小鼠单克隆caspase-3 p17抗体，可通过WB、IP、IF、IHC(P)和ELISA检测到人源caspase-3的p17亚基和全长前体。caspase-3 p17抗体（B-4）既可以是非偶联抗caspase-3 p17抗体的形式，也可以是多种偶联形式的抗caspase-3 p17抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Caspase-3，也称为apopain、SCA-1、Yama和CPP32，是一种天冬氨酸特异性的半胱氨酸蛋白酶，属于caspases的ICE亚家族。Caspase-3在细胞中作为一种无活性的前体表达，在凋亡过程中，成熟的caspase-3的p17和p11亚基从前体中蛋白水解产生。Caspase-3前体首先在Asp 175-Ser 176处被裂解，产生p11亚基和p20肽。随后，p20肽在Asp 28-Ser 29处被裂解，生成成熟的p17亚基。活性caspase-3酶是由两个p17和两个p11亚基组成的异二聚体。在凋亡开始时，caspase-3在Asp 216-Gly 217键处蛋白水解切割PARP。在执行凋亡级联反应过程中，活化的caspase-3以不同于其正常固醇依赖性激活的蛋白水解反应从ER膜上释放SREBP。Caspase-3在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间切割并激活SREBPs。此外，Caspase-3还切割并激活caspase-6、-7和-9。人类caspase-3基因编码一种细胞质蛋白，在肺、脾、心、肝、肾和免疫系统中高度表达。该抗体是研究细胞凋亡、疾病发生机制以及药物筛选等领域的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-3 p17 抗体 (B-4) 参考文献:

  1. 凋亡诱导的成熟固醇调节元件结合蛋白的释放可激活固醇反应基因。  |  Higgins, ME. and Ioannou, YA. 2001. J Lipid Res. 42: 1939-46. PMID: 11734566
  2. 弥漫浸润性星形细胞瘤的凋亡和增殖标志物：17 种分子的图谱分析。  |  Liu, X., et al. 2006. J Neuropathol Exp Neurol. 65: 905-13. PMID: 16957584
  3. 依赖于 Caspase 的细胞凋亡途径的进化过程中出现了灵长类特有的加速现象。  |  Vallender, EJ. and Lahn, BT. 2006. Hum Mol Genet. 15: 3034-40. PMID: 16980329
  4. 3-(3,4,5-三甲氧基苯基)-1-氧代-2-丙烯：一种显示强效多药耐药性逆转和选择性细胞毒性的新型药源。  |  Das, U., et al. 2007. Bioorg Med Chem. 15: 3373-80. PMID: 17383883
  5. 用抗肿瘤药物依托泊苷处理人类白血病细胞株 HL60 后，凋亡相关基因 BCL2, BAX, FAS, caspase-3 和新成员 BCL2L12 的 mRNA 表达发生变化。  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
  6. 脓毒症和脓毒性休克患者的 Caspase-3 活化, Bcl-2 含量和可溶性 FAS-配体与炎症标志物谱无关。  |  Kriebel, F., et al. 2006. Ann N Y Acad Sci. 1090: 168-76. PMID: 17384259
  7. P2X7离子通道刺激ATP在创伤相关细胞因子释放和细胞凋亡中的作用  |  Schneider, EM., et al. 2006. Ann N Y Acad Sci. 1090: 245-52. PMID: 17384268
  8. 鉴定和抑制哺乳动物凋亡所需的 ICE/CED-3 蛋白酶  |  Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
  9. Caspases：细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 p17 抗体 (B-4)

sc-271028
200 µg/ml
RMB2377.00

caspase-3 p17 (B-4): m-IgG Fc BP-HRP 套装

sc-529100
200 µg Ab; 10 µg BP
RMB2662.00

caspase-3 p17 (B-4): m-IgGκ BP-HRP 套装

sc-521819
200 µg Ab, 40 µg BP
RMB2662.00

caspase-3 p17 (B-4): m-IgG2a BP-HRP 套装

sc-547261
200 µg Ab; 10 µg BP
RMB2662.00

caspase-3 p17 抗体 (B-4) AC

sc-271028 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-3 p17 抗体 (B-4) HRP

sc-271028 HRP
200 µg/ml
RMB2377.00

caspase-3 p17 抗体 (B-4) FITC

sc-271028 FITC
200 µg/ml
RMB2482.00

caspase-3 p17 抗体 (B-4) PE

sc-271028 PE
200 µg/ml
RMB2580.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 488

sc-271028 AF488
200 µg/ml
RMB2685.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 546

sc-271028 AF546
200 µg/ml
RMB2685.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 594

sc-271028 AF594
200 µg/ml
RMB2685.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 647

sc-271028 AF647
200 µg/ml
RMB2685.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 680

sc-271028 AF680
200 µg/ml
RMB2685.00

caspase-3 p17 抗体 (B-4) Alexa Fluor® 790

sc-271028 AF790
200 µg/ml
RMB2685.00

Dear Sir/Mam, Does SC-9664 support immunohistochemistry for rat samples? I could not determine this from publications and/or datsheet. Thank you! Mona

Asked by: Mona
Thank you for your question. caspase-3 p17 Antibody (B-4) is only recommended for detection of p17 subunit and full length precursor of caspase-3 of human origin by WB, IP, IF, IHC(P) and ELISA. Based on our tests we do not recommend it for use with rat samples.
Answered by: Tech Support Europe
Date published: 2024-11-28

Is there antibody aganist p17 only without the procaspase-3 for IHC(P)?

Asked by: ManarS
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-22

Can this antibody recognize Caspase 3 after being cleaved? The size is about 17kDa.

Asked by: lidi
Thank you for your question. This monoclonal antibody is recommended for detection of the cleaved p17 subunit and full length precursor of caspase-3 of human origin. There is Western blot data on our website showing detection of the cleaved caspase-3 p17 in Staurosporine treated HeLa whole cell lysates.
Answered by: Technical Support
Date published: 2019-01-25

Is there a recommended positive control for this Ab?

Asked by: Rachie
Thank you for your question. We recommend using the following positive controls with this caspase-3 p17 Antibody (B-4): sc-271028: Untreated (sc-2200) and Staurosporine (sc-3510) treated HeLa (sc-24698) or Jurkat (sc-24719) whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. Untreated (sc-2239) and Kendomycin (sc-202196) treated U-937 whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. CCRF-CEM (sc-2225), Jurkat (sc-2204), K-562 (sc-2203), and Hep G2 (sc-2227) whole cell lysates for detection of procaspase-3 expression in Western blot.
Answered by: Technical Support
Date published: 2017-05-16

Can this antibody also recognize the p12 subunit of caspase 3?

Asked by: AbPolly
Thank you for your question. Our caspase-3 p17 sc-271028 is specific to the precursor form of caspase-3 and the p17 subunit, and will not detect the p12 subunit.
Answered by: Technical Support 2
Date published: 2017-01-24
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Rated 5 out of 5 by from Very good in Western blottingPro and cleaved forms of caspase-3 were detectable in CCCP-treated K562 cells by Western blotting.
Date published: 2019-12-24
Rated 5 out of 5 by from Works Perfectly on WBThis antibody works perfectly on WB analysis, incubation Overnight at 1/2000 in non dry fat milk
Date published: 2018-06-02
Rated 5 out of 5 by from Perfect clean detection 33 kDa (pro-caspase) bandThis Ab lit up in the correct Mw range (33 kDa) very quickly and cleanly. Unfortunately, we were unable to see the p17 fragment. We're currently optimising our cell culture incubations to detect this. Otherwise, very clean WB from this Ab. Would recommend. Conditions: 40 µg protein, 1º Ab 1/200, overnight at RT, 2º Ab, 1/10,000 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) #sc-516102, 2 hrs RT. Imaging: ECL.
Date published: 2017-05-09
Rated 5 out of 5 by from works wellWestern blotting detection of human cancer cells, get the right band.
Date published: 2017-04-05
Rated 5 out of 5 by from Great for ImmunoperoxidaseThis is antibody using Immunoperoxidase staining of human duodenum tissue, and it's very specific and clear.
Date published: 2017-03-28
Rated 5 out of 5 by from WB: cleanWorked great on both our human samples for WB, 1:1000 dilution, very well established antibody.
Date published: 2017-02-12
Rated 5 out of 5 by from Works for detection of p17 subunitThe (B-4) clone works for detection of the p17 subunit by WB in Staurosporine treated samples.
Date published: 2017-01-05
Rated 5 out of 5 by from WonderfulThe memberane was blocked in 5% skim milk and probed with the caspase-3 antibody,the result is as:
Date published: 2016-11-24
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caspase-3 p17 抗体 (B-4) is rated 4.9 out of 5 by 10.
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