Date published: 2025-9-7

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caspase-3 p11 抗体 (C-6): sc-271759

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说明书
  • caspase-3 p11抗体(C-6)是小鼠单克隆IgG1 λ, 在121篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的caspase-3 p11的N-terminus的氨基酸185-211之间
  • 抗-caspase-3 p11 抗体 (C-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 caspase-3 p11 subunit and full length procaspase-3
  • 抗caspase-3 p11抗体(C-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取caspase-3 p11 (C-6): sc-271759免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP)是 caspase-3 p11 抗体 (C-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与caspase-3 p11 抗体 (C-6) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGλ BP 偶联产品,请参阅我们的 小鼠 IgG 结合蛋白完整列表。

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関連項目

caspase-3 p11抗体(C-6)是一种IgG1 λ小鼠单克隆caspase-3 p11抗体,可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的caspase-3 p11亚基和全长procaspase-3。caspase-3 p11抗体(C-6)可以是非偶联抗caspase-3 p11抗体的形式,也可以是多种偶联形式的抗caspase-3 p11抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Caspase-3,也称为apopain、SCA-1、Yama和CPP32,是一种天冬氨酸特异性的半胱氨酸蛋白酶,属于caspases的ICE亚家族。Caspase-3在细胞中作为一种无活性的前体表达,在凋亡过程中,成熟的caspase-3的p17和p11亚基从前体中蛋白水解产生。caspase-3前体首先在Asp 175-Ser 176处被裂解,产生p11亚基和p20肽。随后,p20肽在Asp 28-Ser 29处被裂解,生成成熟的p17亚基。活性caspase-3酶是由两个p17和两个p11亚基组成的异二聚体。在凋亡开始时,caspase-3在Asp 216-Gly 217键处蛋白水解切割PARP。在执行凋亡级联反应过程中,活化的caspase-3以不同于其正常固醇依赖性激活的蛋白水解反应从ER膜上释放SREBP。Caspase-3在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间切割并激活SREBPs。Caspase-3还切割并激活caspase-6、-7和-9。人类caspase-3基因编码一种细胞质蛋白,在肺、脾、心、肝、肾和免疫系统中高度表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-3 p11 抗体 (C-6) 参考文献:

  1. 凋亡诱导的成熟固醇调节元件结合蛋白的释放可激活固醇反应基因。  |  Higgins, ME. and Ioannou, YA. 2001. J Lipid Res. 42: 1939-46. PMID: 11734566
  2. 弥漫浸润性星形细胞瘤的凋亡和增殖标志物:17 种分子的图谱分析。  |  Liu, X., et al. 2006. J Neuropathol Exp Neurol. 65: 905-13. PMID: 16957584
  3. 依赖于 Caspase 的细胞凋亡途径的进化过程中出现了灵长类特有的加速现象。  |  Vallender, EJ. and Lahn, BT. 2006. Hum Mol Genet. 15: 3034-40. PMID: 16980329
  4. 3-(3,4,5-三甲氧基苯基)-1-氧代-2-丙烯:一种显示强效多药耐药性逆转和选择性细胞毒性的新型药源。  |  Das, U., et al. 2007. Bioorg Med Chem. 15: 3373-80. PMID: 17383883
  5. 用抗肿瘤药物依托泊苷处理人类白血病细胞株 HL60 后,凋亡相关基因 BCL2, BAX, FAS, caspase-3 和新成员 BCL2L12 的 mRNA 表达发生变化。  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
  6. 脓毒症和脓毒性休克患者的 Caspase-3 活化, Bcl-2 含量和可溶性 FAS-配体与炎症标志物谱无关。  |  Kriebel, F., et al. 2006. Ann N Y Acad Sci. 1090: 168-76. PMID: 17384259
  7. P2X7离子通道刺激ATP在创伤相关细胞因子释放和细胞凋亡中的作用  |  Schneider, EM., et al. 2006. Ann N Y Acad Sci. 1090: 245-52. PMID: 17384268
  8. 鉴定和抑制哺乳动物凋亡所需的 ICE/CED-3 蛋白酶  |  Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
  9. Caspases:细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 p11 抗体 (C-6)

sc-271759
200 µg/ml
RMB2377.00

caspase-3 p11 (C-6): m-IgGλ BP-HRP 套装

sc-522052
200 µg Ab, 40 µg BP
RMB2662.00

caspase-3 p11 抗体 (C-6) AC

sc-271759 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-3 p11 抗体 (C-6) HRP

sc-271759 HRP
200 µg/ml
RMB2377.00

caspase-3 p11 抗体 (C-6) FITC

sc-271759 FITC
200 µg/ml
RMB2482.00

caspase-3 p11 抗体 (C-6) PE

sc-271759 PE
200 µg/ml
RMB2580.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 488

sc-271759 AF488
200 µg/ml
RMB2685.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 546

sc-271759 AF546
200 µg/ml
RMB2685.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 594

sc-271759 AF594
200 µg/ml
RMB2685.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 647

sc-271759 AF647
200 µg/ml
RMB2685.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 680

sc-271759 AF680
200 µg/ml
RMB2685.00

caspase-3 p11 抗体 (C-6) Alexa Fluor® 790

sc-271759 AF790
200 µg/ml
RMB2685.00

caspase-3 p11 (C-6) 中和勝肽

sc-271759 P
100 µg/0.5 ml
RMB511.00

Hi, can you tell me if this mouse antibody works on mouse tissue for Immunohistochemistry. Thanks!

Asked by: IHC 1
Thank you for your question. Yes, this mouse monoclonal antibody, caspase-3 p11 (C-6): sc-271759, can be used for immunohistochemistry or IHC(P) on mouse tissue. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-08-18

Is caspase-3 p11 (C-6), sc-271759, available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Thank you for your question! Yes, sc-271759 is available conjugated to agarose (sc-271759 AC), HRP (sc-271759 HRP), phycoerythrin (sc-271759 PE), fluorescein (sc-271759 FITC), Alexa Fluor® 488 (sc-271759 AF488) and Alexa Fluor® 647 (sc-271759 AF647).
Answered by: Technical Support 13
Date published: 2017-01-13

What is the best secondary to use with this antibody in Western Blot?

Asked by: TinTin
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-10
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Rated 4 out of 5 by from Efficient stainingI try this antibody in a immunoflourescence for C6 cells with pretty good results.
Date published: 2020-01-13
Rated 5 out of 5 by from Western blotWhen tried 1:500 dilution,tried in human spleen tissue and the effect of this antibody was good, it always good band in 40kDa.
Date published: 2018-05-26
Rated 5 out of 5 by from Good productionThe product quality is very good, and the result of the band was very clear and strong signal.as we expected!
Date published: 2018-03-01
Rated 4 out of 5 by from good antibodyStrong fast signal.. BUT with many unspecific band
Date published: 2017-12-21
Rated 4 out of 5 by from Works fineThe antibody works fine, but shows some nonspecific bands in my sample.
Date published: 2017-11-17
Rated 4 out of 5 by from IHC Staining of Human Adenocarcinoma CellsI used this antibody to assess apoptosis in human adenocarcinoma cells using IHC. I was satisfied with it's performance and hope to use it in other applications in the future.
Date published: 2017-10-20
Rated 2 out of 5 by from weak bandsyou can see weak bands with this antibody. The sample is human cell line. Primary antibodies are diluted at 1:2000. The PH value of 1*TBS is 7.45.
Date published: 2017-09-30
Rated 5 out of 5 by from anticorps de grande qualitéJ'ai demandé des échantillons d'anticorps à tester. Les produits ont été livrés en moins de 24 heures. la communication est très claire et les informations sont obtenues immédiatement. des anticorps de grande qualité.
Date published: 2017-09-28
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caspase-3 p11 抗体 (C-6) is rated 4.3 out of 5 by 26.
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