Date published: 2025-9-8
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caspase-3 Antibody (E-8) - Western Blotting - Image 395848
caspase-3 抗体 (E-8) Alexa Fluor® 790: sc-7272 AF790. 对 MOLT-4 (A), SUP-T1 (B) 和 BJAB (C) 全细胞裂解液中的 caspase-3 表达进行直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
caspase-3 抗体 (E-8) Alexa Fluor® 790: sc-7272 AF790. 对 MOLT-4 (A), SUP-T1 (B) 和 BJAB (C) 全细胞裂解液中的 caspase-3 表达进行直接近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.

caspase-3 抗体 (E-8): sc-7272

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说明书
  • caspase-3 抗体 E-8 是小鼠单克隆 IgG2a κ，caspase-3抗体, 在792篇文献中引用，规格为200 µg/ml
  • 与代表human起源的caspase-3全长前体形式的氨基酸1-277杂交
  • caspase-3 抗体 (E-8) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测human 来源的 caspase-3 and full length procaspase-3
  • 抗caspase-3抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取caspase-3 (E-8): sc-7272的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 caspase-3 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与caspase-3 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Caspase抗体，包括caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5和caspase-6

Caspase-3抗体（E-8）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测人类caspase-3蛋白，也称为apopain、SCA-1、Yama和CPP32。半胱天冬酶-3单克隆抗体（E-8）针对半胱天冬酶-3的全长前体形式，包括1-277个氨基酸，推荐用于蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（包括石蜡包埋样本）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。Caspase-3是细胞凋亡通路中关键的执行蛋白酶，负责切割PARP等关键底物，从而在程序性细胞死亡过程中有序地分解细胞成分。激活非活性前体蛋白酶-3（procaspase-3）中的蛋白酶-3需要蛋白水解，从而形成由p17和p11亚基组成的功能性异二聚体，这对凋亡信号的传播至关重要。蛋白酶-3不仅通过消除受损或多余细胞来维持细胞内环境，还能激活其他蛋白酶，包括蛋白酶-6、-7和-9，从而放大凋亡级联反应。人caspase-3基因在肺、脾、心、肝、肾和免疫细胞等关键器官中广泛表达，凸显其在各种生理过程中的重要性。抗半胱天冬酶-3单克隆抗体（E-8）有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物，为研究人员提供了多种选择。半胱天冬酶-3（E-8）抗体推荐用于研究细胞凋亡和相关细胞机制，可用于癌症生物学、免疫学和神经退行性疾病等领域的研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

caspase-3 抗体 (E-8) 参考文献:

  1. 凋亡诱导的成熟固醇调节元件结合蛋白的释放可激活固醇反应基因。  |  Higgins, ME. and Ioannou, YA. 2001. J Lipid Res. 42: 1939-46. PMID: 11734566
  2. 弥漫浸润性星形细胞瘤的凋亡和增殖标志物：17 种分子的图谱分析。  |  Liu, X., et al. 2006. J Neuropathol Exp Neurol. 65: 905-13. PMID: 16957584
  3. 依赖于 Caspase 的细胞凋亡途径的进化过程中出现了灵长类特有的加速现象。  |  Vallender, EJ. and Lahn, BT. 2006. Hum Mol Genet. 15: 3034-40. PMID: 16980329
  4. 3-(3,4,5-三甲氧基苯基)-1-氧代-2-丙烯：一种显示强效多药耐药性逆转和选择性细胞毒性的新型药源。  |  Das, U., et al. 2007. Bioorg Med Chem. 15: 3373-80. PMID: 17383883
  5. 用抗肿瘤药物依托泊苷处理人类白血病细胞株 HL60 后，凋亡相关基因 BCL2, BAX, FAS, caspase-3 和新成员 BCL2L12 的 mRNA 表达发生变化。  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
  6. 脓毒症和脓毒性休克患者的 Caspase-3 活化, Bcl-2 含量和可溶性 FAS-配体与炎症标志物谱无关。  |  Kriebel, F., et al. 2006. Ann N Y Acad Sci. 1090: 168-76. PMID: 17384259
  7. P2X7离子通道刺激ATP在创伤相关细胞因子释放和细胞凋亡中的作用  |  Schneider, EM., et al. 2006. Ann N Y Acad Sci. 1090: 245-52. PMID: 17384268
  8. 鉴定和抑制哺乳动物凋亡所需的 ICE/CED-3 蛋白酶  |  Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
  9. Caspases：细胞凋亡的刽子手。  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 抗体 (E-8)

sc-7272
200 µg/ml
RMB2377.00

caspase-3 (E-8): m-IgG Fc BP-HRP 套装

sc-525433
200 µg Ab; 10 µg BP
RMB2662.00

caspase-3 抗体 (E-8) AC

sc-7272 AC
500 µg/ml, 25% agarose
RMB3129.00

caspase-3 抗体 (E-8) HRP

sc-7272 HRP
200 µg/ml
RMB2377.00

caspase-3 抗体 (E-8) FITC

sc-7272 FITC
200 µg/ml
RMB2482.00

caspase-3 抗体 (E-8) PE

sc-7272 PE
200 µg/ml
RMB2580.00

caspase-3 抗体 (E-8) Alexa Fluor® 488

sc-7272 AF488
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) Alexa Fluor® 546

sc-7272 AF546
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) Alexa Fluor® 594

sc-7272 AF594
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) Alexa Fluor® 647

sc-7272 AF647
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) Alexa Fluor® 680

sc-7272 AF680
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) Alexa Fluor® 790

sc-7272 AF790
200 µg/ml
RMB2685.00

caspase-3 抗体 (E-8) TRITC

sc-7272 TRITC
200 µg/ml
RMB2407.00

caspase-3 抗体 (E-8) Alexa Fluor® 405

sc-7272 AF405
200 µg/ml
RMB2685.00

Which species has this antibody been applied to? Which species does it work for, and which not?

Asked by: DABB
Thanks for your question. The caspase-3 (E-8) antibody is recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA. Please contact Technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I used m-IgGκ BP-HRP: sc-516102 as the secondary antibody, but the bands didn`t existed. The reason is that m-IgGκ BP-HRP: sc-516102 didn`t binded to caspase-3  (E-8): sc-7272, isn`t it ?

Asked by: jmjflying918
Thank you for your question. There are several possibilities that could explain a negative result in Western blot. In this case, one possibility might be that this primary antibody, caspase-3 Antibody (E-8) is a mouse monoclonal with a lambda light chain. If that is the case, then m-IgGκ BP-HRP: sc-516102 would not work. Instead, we would recommend using m-IgGλ BP-HRP: sc-516132 which specifically binds to the lambda light chain of mouse immunoglobulins. We are testing sc-7272 now and will update our product information shortly to specify whether or not this antibody has a kappa or lambda light chain. In the meantime, please contact our Technical Support Department and we would be happy to continue troubleshooting the antibody. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-06

How should I go about double staining a tissue with this antibody if my other primary antibody is also anti-mouse?

Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, caspase-3 Antibody (E-8): sc-7272 is available directly conjugated to HRP (sc-7272 HRP), phycoerythrin (sc-7272 PE), fluorescein (sc-7272 FITC), Alexa Fluor® 488 (sc-7272 AF488), Alexa Fluor® 647 (sc-7272 AF647), TRITC (sc-7272 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-7272 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-28
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Rated 1 out of 5 by from This antibody did not workThis antibody did not work with human cancer cell lines.
Date published: 2025-08-25
Rated 5 out of 5 by from Ótimo produtoPedi uma amostra de casp-3 e funcionou perfeitamente.
Date published: 2021-01-08
Rated 4 out of 5 by from buon anticorpoQuesto anticorpo dà un bel segnale, netto e specifico.
Date published: 2020-11-04
Rated 1 out of 5 by from No detectable in adipose tissue of miceCaspase3 protein was no detectable in subcutaneous white adipose tissue at the position of proper size.
Date published: 2020-01-27
Rated 4 out of 5 by from Excellent.This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 1 out of 5 by from Unspecific bandsIn WB, we got multiple bands, non of which was at the expected size.
Date published: 2018-03-15
Rated 3 out of 5 by from Band intensity in weekAntibody worked but the band intensity is week. maybe I should loaded more protein (usually I load 40-50uG).
Date published: 2018-02-01
Rated 5 out of 5 by from Works great for FFPE IHCWe got this as a sample. This is an easy, nice working antibody to use. HIER sodium citrate pH 6.0, 1:100 on human heart, and appendix. Looks great!
Date published: 2018-01-04
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caspase-3 抗体 (E-8) is rated 4.3 out of 5 by 49.
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