Date published: 2025-9-8

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caspase-3 抗体 (4.1.18): sc-65497

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说明书
  • caspase-3抗体(4.1.18)是小鼠单克隆IgG2a κ, 在49篇文献中引用,规格为200 µg/ml
  • 针对全长caspase-3,human起源
  • 推荐用于 mouse, rat 和 human 来源的caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 caspase-3 p11 (C-6): sc-271759,可查看有标记的 caspase-3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 caspase-3 抗体 (4.1.18) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-3 抗体 (4.1.18) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

caspase-3 Antibody (4.1.18) 是一种小鼠单克隆 IgG2a kappa 轻链抗体,设计用于检测小鼠、大鼠和人体内的活性 caspase-3 及其非活性前体 procaspase-3。Caspase-3 又称 apopain、SCA-1、Yama 和 CPP32,是一种重要的天冬氨酸特异性半胱氨酸蛋白酶,属于 Caspase 的 ICE 亚家族,具有明确的功能所必需的结构域。该蛋白结构由一个大亚基(p17)和一个小亚基(p11)组成,在非活性前体被蛋白水解裂解后形成活性异构四聚体。这种结构构型对 caspase-3 在细胞凋亡中的作用至关重要,它可以高效地裂解各种底物,从而在细胞程序性死亡过程中有序地分解细胞成分。活性位点的精确排列确保了蛋白水解活性的特异性和调节性,使 Caspase-3 成为细胞凋亡途径中的关键执行者。了解 caspase-3 结构的复杂性对于癌症、神经变性和免疫反应等领域的研究至关重要,因为在这些领域中细胞凋亡发挥着重要作用。 18)单克隆抗体可通过免疫印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附试验(ELISA)等应用进行可靠的检测和定量。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 抗体 (4.1.18)

sc-65497
200 µg/ml
RMB2377.00

caspase-3 (4.1.18): m-IgG Fc BP-HRP 套装

sc-539178
200 µg Ab; 10 µg BP
RMB2662.00

caspase-3 (4.1.18): m-IgG2a BP-HRP 套装

sc-546680
200 µg Ab; 10 µg BP
RMB2662.00

Does it function to detect cleaved caspase 3?

Asked by: Dr Inês
Thank you for your question. We have used this mouse monoclonal antibody, caspase-3 (4.1.18): sc-65497, to successfully detect procaspase-3. We do not yet have data with this clone detecting cleaved caspase-3, but the optimal conditions for detection of cleaved caspase-3 may not yet be determined. This antibody has been cited in the literature at least 14 times. We would recommend consulting some of the product citations to see if anyone has used this clone for detection of the cleaved caspase-3.
Answered by: Technical Support
Date published: 2017-06-01

I am using caspase-3 (4.1.18): sc-65497 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from PerfectI used this AB in WB and work perfectly, clear and nice bands.
Date published: 2019-05-13
Rated 5 out of 5 by from Well-rounded antibody for caspase-3Works great for both WB and IHC on paraffin embedded tissue sections.
Date published: 2017-01-18
Rated 5 out of 5 by from Good for Western with human cell lysatesGood for Western with human cell lysates -SCBT QC
Date published: 2014-12-04
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caspase-3 抗体 (4.1.18) is rated 5.0 out of 5 by 3.
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