Date published: 2025-9-7
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caspase-3 Antibody (31A1067) - Western Blotting - Image 17679
caspase-3 抗体 (31A1067): sc-56053. CCRF-CEM (A), HuT 78 (B), CCRF-HSB-2 (C), PC-3 (D), HeLa (E) 和 U-698-M (F) 全细胞裂解液中 caspase-3 表达的 Western 印迹分析.
caspase-3 抗体 (31A1067): sc-56053. CCRF-CEM (A), HuT 78 (B), CCRF-HSB-2 (C), PC-3 (D), HeLa (E) 和 U-698-M (F) 全细胞裂解液中 caspase-3 表达的 Western 印迹分析.

caspase-3 抗体 (31A1067): sc-56053

4.5(28)
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说明书
  • caspase-3 抗体 31A1067 是小鼠单克隆 IgG1, 在297篇文献中引用，提供 50 µg/0.5 ml
  • 针对全长caspase-3，human起源
  • 推荐用于 mouse, rat 和 human 来源的caspase-3 and full length procaspase-3 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 caspase-3 p11 (C-6): sc-271759，可查看有标记的 caspase-3 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取caspase-3 (31A1067): sc-56053的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 caspase-3 抗体 (31A1067) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与caspase-3 抗体 (31A1067) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Caspase抗体，包括caspase-1, caspase-10, caspase-11, caspase-12, caspase-14, caspase-2, caspase-3, caspase-4, caspase-5, caspase-6, caspase-7, caspase-8和caspase-9

caspase-3抗体（31A1067）是一种IgG1小鼠单克隆caspase-3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的caspase-3和全长procaspase-3。caspase-3抗体（31A1067）以非偶联抗caspase-3抗体的形式提供。Caspase-3，也称为apopain、SCA-1、Yama和CPP32，是一种天冬氨酸特异性的半胱氨酸蛋白酶，属于caspases的ICE亚家族。Caspase-3在细胞中作为一种无活性的前体表达，在凋亡过程中，成熟的caspase-3的p17和p11亚基从前体中蛋白水解产生。caspase-3前体首先在Asp175-Ser176处被裂解，产生p11亚基和p20肽。随后，p20肽在Asp28-Ser29处被裂解，生成成熟的p17亚基。活性caspase-3酶是由两个p17和两个p11亚基组成的异二聚体。在凋亡开始时，caspase-3在Asp216-Gly217键处蛋白水解切割PARP。在执行凋亡级联反应过程中，活化的caspase-3以不同于其正常固醇依赖性激活的蛋白水解反应从ER膜上释放SREBP。Caspase-3在碱性螺旋-环-螺旋亮氨酸拉链结构域和膜附着结构域之间切割并激活SREBPs。Caspase-3还切割并激活caspase-6、-7和-9。人类caspase-3基因编码一种细胞质蛋白，在肺、脾、心、肝、肾和免疫系统中高度表达。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

caspase-3 抗体 (31A1067)

sc-56053
50 µg/0.5 ml
RMB2377.00

caspase-3 (31A1067): m-IgG Fc BP-HRP 套装

sc-539027
50 µg Ab; 10 µg BP
RMB2662.00

caspase-3 (31A1067): m-IgG1 BP-HRP 套装

sc-541215
50 µg Ab; 20 µg BP
RMB2662.00

I could not detect specific band on following condition on my western blot. ･Nitrocellulose membrane 0.45 µm ･C2C12 myoblast and C2C12 myoblast + H2O2 (500mM) ･Caspase-3 antibody: 1:200 ･IgGk antibody: 1:1000 Could you please give me some advice?

Asked by: SJWD
Thank you for your question.Please contact our Technical Service Department of Japan for more information.
Answered by: Jiajun Z
Date published: 2022-10-27

Can you recommend a compatible diluent buffer and concentration for this antibody? My blot showed up with a strong background and I was wondering what need to be modified?

Asked by: Saina
Thank you for your question. This antibody was established using UltraCruz® Blocking Reagent: sc-516214, which is a highly efficient plant protein based blocking buffer. You may also want to increase incubation time of the primary antibody to overnight incubation at +4° C, which allows for lowering the overall primary antibody concentration, and in consequence unspecific signals. If the issue persists, you may also want to consider using one of our secondary binding proteins, which circumvent unspecific signals from secondary detection in comparison to conventional polyclonal secondaries.
Answered by: Tech Support Europe
Date published: 2022-06-20

caspase-3 Antibody (31A1067): sc-56053 is IgG1 kappa light chain?

Asked by: pili1234
Thank you for your question. Unfortunately we do not have isotype information for this antibody at this time. Most likely it is kappa, but we can not say for sure.
Answered by: Tech Service
Date published: 2020-02-07

Est-ce que je peux avoir un devis pour l'achat de l'anticorps caspase-3 (31A1067): sc-56053; Merci

Asked by: Hadj
Merci pour votre question. Veuillez contacter directement notre service clientèle et nous vous ferons parvenir immédiatement un devis pour ce produit. 'Please send me a quote for caspase-3 Antibody (31A1067): sc-56053.' Thank you for your question. Please contact our customer service directly and we will send you a quotation for this product right away.
Answered by: Technical Support Europe
Date published: 2019-04-12

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-20
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Rated 4 out of 5 by from Great AbI test this antibody conjugated with AF594, and it works very well.
Date published: 2025-03-31
Rated 5 out of 5 by from Great for WBIt is a good antibody for western blot. I'd recommend it.
Date published: 2024-03-20
Rated 4 out of 5 by from Good bandswe used it in mouse gut tissues, 1:500 dilution, mosttimes we can get pro- and mature-cas3 bands
Date published: 2024-01-10
Rated 5 out of 5 by from opinions about antibodiesVery good antibodies, work at delal dilutions, no non-specific binding
Date published: 2023-05-24
Rated 5 out of 5 by from 质量不错我两年前买了这管抗体，目前研究工作已经发表Bioactive Materials，标题为DNA aptamer S11e recognizes fibrosarcoma and acts as a tumor suppressor。值得推荐！
Date published: 2022-03-13
Rated 3 out of 5 by from Buena señalLa señal es buena, pero algo sucia. La banda no sale bien definida
Date published: 2021-11-11
Rated 3 out of 5 by from Discreto funzionamentoHo comprato questo articolo 5 settimane fa. Provando condizioni diverse di wb sono riuscita ad ottenere un discreto segnale di bande
Date published: 2021-02-12
Rated 4 out of 5 by from Questions about the free samples(31A1067)The free samples(31A1067) can only detect procaspase-3. We can't detect the cleaved caspase-3 as you showed in the products specification. Do you offer specific antibody for it?
Date published: 2020-06-10
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caspase-3 抗体 (31A1067) is rated 4.5 out of 5 by 28.
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