Date published: 2025-9-6
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casein kinase IIα Antibody (1AD9) - Western Blotting - Image 2191
酪蛋白激酶 IIα (1AD9): sc-12738. 小鼠脑组织提取物 (A) 和 PC-12 全细胞裂解液 (B) 中酪蛋白激酶 IIα 表达的 Western 印迹分析.
酪蛋白激酶 IIα (1AD9): sc-12738. 小鼠脑组织提取物 (A) 和 PC-12 全细胞裂解液 (B) 中酪蛋白激酶 IIα 表达的 Western 印迹分析.

casein kinase IIα 抗体 (1AD9): sc-12738

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说明书
  • casein kinase IIα抗体(1AD9)是小鼠单克隆IgG1 κ, 在48篇文献中引用，规格为200 µg/ml
  • 针对纯化的casein kinase IIα human 起源
  • 抗-casein kinase II alpha 抗体 (1AD9) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 casein kinase IIα; 也和以下物种反应，包括： bovine
  • 抗casein kinase II alpha抗体（1AD9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取casein kinase IIα (1AD9): sc-12738的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 casein kinase IIα 抗体 (1AD9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与casein kinase IIα 抗体 (1AD9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Casein Kinase 抗体 包括 casein kinase I α, casein kinase I δ, casein kinase I ε, casein kinase I γ 1, casein kinase I γ 2, casein kinase II α, casein kinase II α prime和casein kinase II β

酪蛋白激酶IIα抗体（1AD9）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测酪蛋白激酶IIα蛋白，也称为CK2α或CSNK2A1。酪蛋白激酶IIα单克隆抗体（1AD9）针对纯化的人酪蛋白激酶IIα产生，可用于多种应用，包括多物种（如小鼠、大鼠、人和牛）的蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。酪蛋白激酶IIα是一种重要的丝氨酸/苏氨酸蛋白激酶，参与调节细胞周期的进展、DNA复制和修复、细胞凋亡和转录调控等多种细胞过程。酪蛋白激酶IIα对于维持细胞内环境平衡至关重要，在癌症中经常出现失调，因此CK2α成为肿瘤发生和其他增生性疾病研究的重要靶标。CK2α亚基通常与调节性β亚基形成四聚体复合物，通过自磷酸化增强催化功能。CK2α通过磷酸化100多种底物（包括核癌蛋白和转录因子）在细胞信号传导通路和基因表达中发挥关键作用。酪蛋白激酶IIα（1AD9）抗体有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®变体，可满足各种实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

casein kinase IIα 抗体 (1AD9) 参考文献:

  1. 酪蛋白激酶 I 和 II--多潜在丝氨酸蛋白激酶：结构, 功能和调节。  |  Tuazon, PT. and Traugh, JA. 1991. Adv Second Messenger Phosphoprotein Res. 23: 123-64. PMID: 1997039
  2. 编码酪蛋白激酶 II α 和 α' 亚基的人类 cDNA 克隆的分离和特征。  |  Lozeman, FJ., et al. 1990. Biochemistry. 29: 8436-47. PMID: 2174700
  3. 酪蛋白激酶 I γ 亚家族。三种哺乳动物同工酶的分子克隆, 表达和特征以及对酿酒酵母 YCK 基因缺陷的互补。  |  Zhai, L., et al. 1995. J Biol Chem. 270: 12717-24. PMID: 7759525
  4. 人类酪蛋白激酶 I epsilon（CKI）--CKI 基因家族的新成员--的分离和特征描述。  |  Fish, KJ., et al. 1995. J Biol Chem. 270: 14875-83. PMID: 7797465
  5. 蛋白激酶4.蛋白激酶 CK2：一种具有多种底物和令人费解的调节的酶。  |  Allende, JE. and Allende, CC. 1995. FASEB J. 9: 313-23. PMID: 7896000
  6. 酪蛋白激酶 II 在信号转导和细胞周期调节中的作用  |  Litchfield, DW. and Lüscher, B. 1993. Mol Cell Biochem. 127-128: 187-99. PMID: 7935350
  7. 大鼠睾丸中 49 千道尔顿酪蛋白激酶 I 同工酶的分子克隆, 表达和表征。  |  Graves, PR., et al. 1993. J Biol Chem. 268: 6394-401. PMID: 8454611

订购信息

产品名称产品编号规格价格数量收藏夹

casein kinase IIα 抗体 (1AD9)

sc-12738
200 µg/ml
RMB2377.00

casein kinase IIα (1AD9): m-IgG Fc BP-HRP 套装

sc-528254
200 µg Ab; 10 µg BP
RMB2662.00

casein kinase IIα (1AD9): m-IgGκ BP-HRP 套装

sc-520585
200 µg Ab, 40 µg BP
RMB2662.00

casein kinase IIα (1AD9): m-IgG1 BP-HRP 套装

sc-542843
200 µg Ab; 20 µg BP
RMB2662.00

casein kinase IIα 抗体 (1AD9) AC

sc-12738 AC
500 µg/ml, 25% agarose
RMB3129.00

casein kinase IIα 抗体 (1AD9) HRP

sc-12738 HRP
200 µg/ml
RMB2377.00

casein kinase IIα 抗体 (1AD9) FITC

sc-12738 FITC
200 µg/ml
RMB2482.00

casein kinase IIα 抗体 (1AD9) PE

sc-12738 PE
200 µg/ml
RMB2580.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 488

sc-12738 AF488
200 µg/ml
RMB2685.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 546

sc-12738 AF546
200 µg/ml
RMB2685.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 594

sc-12738 AF594
200 µg/ml
RMB2685.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 647

sc-12738 AF647
200 µg/ml
RMB2685.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 680

sc-12738 AF680
200 µg/ml
RMB2685.00

casein kinase IIα 抗体 (1AD9) Alexa Fluor® 790

sc-12738 AF790
200 µg/ml
RMB2685.00

Hello, I would like to inquire about the primary antibodies for CK2A1, CK2A2, and CK2B, but it seems that your company only has CK2α. Could you please tell me whether this CK2α antibody is CK2A1 or CK2A2?

Asked by: Zhao
Thank you for your question. This antibody is only recommended for the detection of CK2A1. Thank you.
Answered by: Jiajun Z
Date published: 2024-12-24

which secondary antibody to be used for IF with this antibody. I am getting no result. i used sc-12738 antibody

Asked by: priyara
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-10-12

What is the recommended secondary antibody for WB, for use with this monoclonal antibody?

Asked by: Anna99
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-12

Can this mouse monoclonal antibody be used to stain mouse tissue?

Asked by: Anna99
While this antibody is recommended for detection in mouse, it is not available as a direct conjugate at this time. See casein kinase IIα (E-7): sc-373894 for casein kinase IIα antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Technical Support 7
Date published: 2017-01-12
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Rated 5 out of 5 by from Great for WBIt is a great antibody for Western blot using human cell nuclear extract.
Date published: 2018-09-15
Rated 5 out of 5 by from Quite clear band for wb of rat protein1:500 diluted for wb of rat protein which extracted from h9c2 cell,the band is special and strong.
Date published: 2017-08-28
Rated 5 out of 5 by from perfectI employed it for IHC in human adrenal gland tissue, the result shows strong staining
Date published: 2017-05-11
Rated 5 out of 5 by from Great IF images using this antibodyWe suggest 1:50 dilution in IF, It works very well
Date published: 2017-04-17
Rated 5 out of 5 by from Great productOutstanding data in IHC staining and western blot studies
Date published: 2017-01-19
Rated 5 out of 5 by from Good Ab for IHCThis antibody has good results for IHC staining in human tissue
Date published: 2017-01-11
Rated 5 out of 5 by from Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-05
Rated 5 out of 5 by from Strong band in WB in human transfected 293TStrong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-08
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casein kinase IIα 抗体 (1AD9) is rated 5.0 out of 5 by 10.
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