Date published: 2025-9-10
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Cas9 Antibody (7A9-3A3) - Western Blotting - Image 384064
Cas9 抗体 (7A9-3A3): sc-517386. 在非转染: sc-117752 (A) 和化脓性链球菌 Cas9 转染 (B, C 和 D) 的 293T 全细胞裂解液中进行 Cas9 表达的 Western 印迹分析. 使用的 CRISPR/Cas9 质粒: sc-400099 (B), sc-400193 (C) 和 sc-413603 (D).
Cas9 Antibody (7A9-3A3) - Western Blotting - Image 384064
Cas9 抗体 (7A9-3A3): sc-517386. 在非转染: sc-117752 (A) 和化脓性链球菌 Cas9 转染 (B, C 和 D) 的 293T 全细胞裂解液中进行 Cas9 表达的 Western 印迹分析. 使用的 CRISPR/Cas9 质粒: sc-400099 (B), sc-400193 (C) 和 sc-413603 (D).
Cas9 Antibody (7A9-3A3) - Western Blotting - Image 395718
Cas9 抗体 (7A9-3A3) HRP: sc-517386 HRP. 在非转染: sc-117752 (A) 和化脓性链球菌 Cas9 转染 (B 和 C) 的 293T 全卖裂解液中对 Cas9 表达的直接 Western 印迹分析. 使用的 CRISPR/Cas9 质粒: sc-400193 (B) 和 sc-413603 (C).
Cas9 抗体 (7A9-3A3): sc-517386. 在非转染: sc-117752 (A) 和化脓性链球菌 Cas9 转染 (B, C 和 D) 的 293T 全细胞裂解液中进行 Cas9 表达的 Western 印迹分析. 使用的 CRISPR/Cas9 质粒: sc-400099 (B), sc-400193 (C) 和 sc-413603 (D).

Cas9 抗体 (7A9-3A3): sc-517386

4.8(6)
寫評論提問

说明书
  • Cas9 抗体 7A9-3A3 是小鼠单克隆 IgG1 κ，Cas9抗体, 在31篇文献中引用，规格为200 µg/ml
  • 针对与N-terminal区域对应的重组蛋白Cas9的氨基酸Streptococcus pyogenes的氨基酸
  • Cas9 抗体 (7A9-3A3) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测Streptococcus pyogenes 来源的 transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9)
  • 抗Cas9抗体（7A9-3A3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cas9 (7A9-3A3): sc-517386的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Cas9 抗体 (7A9-3A3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cas9 抗体 (7A9-3A3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
说明书与实验方案
研究信息

関連項目

Cas9抗体（7A9-3A3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测链球菌源的Cas9蛋白。抗Cas9抗体（7A9-3A3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Cas9是一种由1368个氨基酸组成的RNA引导的内切酶，在CRISPR-Cas9基因编辑系统中起着至关重要的作用，能够对DNA序列进行精确的修改。Cas9能够在特定的基因组位置产生双链断裂，这对于靶向基因破坏至关重要，使研究人员能够研究基因功能并开发潜在的治疗策略。Cas9的结构包括识别臂和核酸酶臂，它们对于与指导RNA和目标DNA的相互作用至关重要，从而确保基因编辑应用中的高特异性和高效率。抗Cas9抗体（7A9-3A3）是分子生物学、生物技术和治疗开发中的有力工具，为遗传研究和遗传疾病治疗的发展铺平了道路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cas9 参考文献:

  1. CRISPR/Cas 系统及其在噬菌体与细菌相互作用中的作用。  |  Deveau, H., et al. 2010. Annu Rev Microbiol. 64: 475-93. PMID: 20528693
  2. CRISPR RNA 通过反式编码的小 RNA 和宿主因子 RNase III 成熟。  |  Deltcheva, E., et al. 2011. Nature. 471: 602-7. PMID: 21455174
  3. 适应性细菌免疫中的可编程双 RNA 导向 DNA 内切酶  |  Jinek, M., et al. 2012. Science. 337: 816-21. PMID: 22745249
  4. Cas9-crRNA核糖核蛋白复合物介导特异性DNA切割，促进细菌的适应性免疫。  |  Gasiunas, G., et al. 2012. Proc Natl Acad Sci U S A. 109: E2579-86. PMID: 22949671
  5. CRISPR RNA 引导的内切酶 Cas9 的 DNA 检测。  |  Sternberg, SH., et al. 2014. Nature. 507: 62-7. PMID: 24476820
  6. Cas9 与引导 RNA 和目标 DNA 复合物的晶体结构。  |  Nishimasu, H., et al. 2014. Cell. 156: 935-49. PMID: 24529477
  7. 用于基因组工程的 CRISPR-Cas9 的开发和应用。  |  Hsu, PD., et al. 2014. Cell. 157: 1262-1278. PMID: 24906146
  8. 揭示 CRISPR-Cas 系统的结构和机理基础。  |  van der Oost, J., et al. 2014. Nat Rev Microbiol. 12: 479-92. PMID: 24909109
  9. [与麻醉过程有关的淋巴细胞亚群变化]。  |  Käbisch, S., et al. 1986. Anasth Intensivther Notfallmed. 21: 327-32. PMID: 2949671

订购信息

产品名称产品编号规格价格数量收藏夹

Cas9 抗体 (7A9-3A3)

sc-517386
200 µg/ml
RMB2377.00

Cas9 (7A9-3A3): m-IgG Fc BP-HRP 套装

sc-531580
200 µg Ab; 10 µg BP
RMB2662.00

Cas9 (7A9-3A3): m-IgGκ BP-HRP 套装

sc-525309
200 µg Ab, 40 µg BP
RMB2662.00

Cas9 (7A9-3A3): m-IgG1 BP-HRP 套装

sc-544637
200 µg Ab; 20 µg BP
RMB2662.00

Cas9 抗体 (7A9-3A3) AC

sc-517386 AC
500 µg/ml, 25% agarose
RMB3129.00

Cas9 抗体 (7A9-3A3) HRP

sc-517386 HRP
200 µg/ml
RMB2377.00

Cas9 抗体 (7A9-3A3) FITC

sc-517386 FITC
200 µg/ml
RMB2482.00

Cas9 抗体 (7A9-3A3) PE

sc-517386 PE
200 µg/ml
RMB2580.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 488

sc-517386 AF488
200 µg/ml
RMB2685.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 546

sc-517386 AF546
200 µg/ml
RMB2685.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 594

sc-517386 AF594
200 µg/ml
RMB2685.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 647

sc-517386 AF647
200 µg/ml
RMB2685.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 680

sc-517386 AF680
200 µg/ml
RMB2685.00

Cas9 抗体 (7A9-3A3) Alexa Fluor® 790

sc-517386 AF790
200 µg/ml
RMB2685.00

Could I use this for flow?

Asked by: NilaM
Thank you for your question. Yes, the antibody is recommended for WB, IP, IF, IHC(P) and FCM.
Answered by: BlakeJ
Date published: 2023-08-02

How I could have a free sample for immunofluorescent staining?

Asked by: VcPb
Thank you for your question. To arrange for a free sample of this antibody, please contact our technical service team directly. Please note that only unconjugated antibodies can be supplied as samples.
Answered by: Tech Support Europe
Date published: 2022-12-01

Do you provide sample for us to try? Thank you!

Asked by: Longjun
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-12-20

As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.

Asked by: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03

Is there any validation of this antibody working for immunofluorescence applications?

Asked by: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Answered by: Technical Support
Date published: 2019-03-13

What part of the N-terminus was this antibody raised against?

Asked by: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Answered by: Technical Support
Date published: 2020-12-20
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Rated 5 out of 5 by from Works for Western blotThe antibody is effective for SDS immunoblotting. To dilute the primary antibody, we used a ratio of 1:500 in a mixture of 5% nonfat dry milk, 1X TBS, and 0.1% Tween® 20. The antibody was left overnight at 4°C.
Date published: 2023-11-07
Rated 5 out of 5 by from Good antibody for WBI used 1:1000. It works good with my cells transfected with dCAS9 plasmid.
Date published: 2022-01-25
Rated 5 out of 5 by from This antibody sample worked wellThis cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459. 1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date published: 2018-09-25
Rated 5 out of 5 by from Great antibodyI got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date published: 2018-02-22
Rated 4 out of 5 by from 还不错我一个月前申请可改抗体的试用装，很高兴可以提供试用反馈，这个抗体总体不错，有杂带，但是主带清晰，不受干扰。
Date published: 2017-09-27
Rated 5 out of 5 by from successful resultI have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃，if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
Date published: 2017-07-23
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Cas9 抗体 (7A9-3A3) is rated 4.8 out of 5 by 6.
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