Cas9抗体 (7A9-3A3) 推荐用于 Streptococcus pyogenes 来源的transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9) WB, IP, IF, IHC(P) 和 FCM检测

Cas9 抗体 (7A9-3A3): sc-517386

Cas9抗体 (7A9-3A3) is rated 4.8 out of 5 by 5.
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Datasheets

    • 抗-Cas9 抗体 7A9-3A3 是小鼠单克隆 IgG1 (kappa轻链),Cas9抗体,规格为200 µg/ml
    • 培养自重组蛋白,对应于Streptococcus pyogenes来源的Cas9N-terminal
    • Cas9 抗体 (7A9-3A3) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测Streptococcus pyogenes 来源的 transfected levels of total Cas9 protein; recognizes Cas9 variants including dCas9 (nuclease deficient Cas9)
    • 抗-Cas9 抗体 (7A9-3A3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Cas9 (7A9-3A3): sc-517386免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Do you provide sample for us to try? Thank you!

Asked by: Longjun
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-11-07

As the question above noted, what is the broad region of possible recognition by this antibody? (i.e. something like "AA1-200"). I plan to use this on a split-Cas9 and need to know whether it will be specific for the N-terminal piece.

Asked by: Nate C
Thank you for your question. This antibody was raised against a recombinant protein corresponding to the N-terminal region of Cas9 of Streptococcus pyogenes origin. The exact binding epitope has not been mapped. It recognizes Cas9 variants including dCas9 (nuclease deficient Cas9). If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-04-03

Is there any validation of this antibody working for immunofluorescence applications?

Asked by: diededh
Thank you for your question. Yes, this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has been validated to work for immunofluorescent staining applications. Unfortunately, we do not yet have any published IF data for this clone on our website. However, this clone, (7A9-3A3) has been widely published in the literature for use in a variety of applications, including IF. We do plan to update the data on our website once we have completed testing of the antibody.
Answered by: Technical Support
Date published: 2019-03-13

What part of the N-terminus was this antibody raised against?

Asked by: Phil K
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Cas9 (7A9-3A3): sc-517386, has not yet been determined.
Answered by: Technical Support
Date published: 2017-07-26
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Rated 5 out of 5 by from This antibody sample worked well This cas9 antibody worked well in HEK293T and Hela cells transfected with PX458 or PX459. 1:1000 2h at room temperature with 40ug cell lysate. I think the dilution ration can be much more than 1:1000.
Date published: 2018-09-25
Rated 5 out of 5 by from Great antibody I got a sample and am so happy that work very well for western blotting. I use the ration 1:1500.
Date published: 2018-02-22
Rated 4 out of 5 by from 还不错 我一个月前申请可改抗体的试用装,很高兴可以提供试用反馈,这个抗体总体不错,有杂带,但是主带清晰,不受干扰。
Date published: 2017-09-27
Rated 5 out of 5 by from Works good. It shows a band at around 160 kDa, and some other bands, probably not specific, because I used high concentration of 1:300
Date published: 2017-08-11
Rated 5 out of 5 by from successful result I have used this antibody against cas9 ,got successful result. The antibody needs to be store at 4℃,if store at -20℃ it did not work.Very reliable,for dilution 1:400,good band seen.
Date published: 2017-07-23
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