Date published: 2025-9-6

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CARMIL 抗体 (E-10): sc-365314

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说明书
  • CARMIL 抗体 E-10 是小鼠单克隆 IgG1 κ,CARMIL抗体, 在1篇文献中引用,规格为200 µg/ml
  • 针对human起源的CARMIL内部区域内的氨基酸662-961进行
  • CARMIL 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CARMIL
  • 抗CARMIL抗体(E-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CARMIL (E-10): sc-365314免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 CARMIL 抗体 (E-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CARMIL 抗体 (E-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

CARMIL抗体(E-10)是一种IgG1 κ小鼠单克隆CARMIL抗体(也称为CARMIL抗体),通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源CARMIL蛋白。CARMIL抗体(E-10)既有非偶联的抗CARMIL抗体形式,也有多种偶联的抗CARMIL抗体形式,包括琼脂糖,HRP, PE, FITC和多个Alexa Fluor®偶联物。CARMIL又称富含亮氨酸重复序列16 (LRRC16),是近年来发现的一个富含亮氨酸重复序列蛋白家族的成员,该家族在全身具有多种功能。CARMIL与Arp2/3复合体、肌动蛋白加帽蛋白CP和肌球蛋白I相互作用,帮助组装对正常细胞发育至关重要的多蛋白结构。通过与这三种蛋白的相互作用,CARMIL可以调节倒刺端肌动蛋白微丝的帽化、Arp2/3复合体介导的肌动蛋白成核以及Myosin I介导的肌动蛋白微丝的组装。这一复合体共同产生各种细胞运动的力量,如胞质分裂、吞噬细胞作用和肌肉收缩。编码CARMIL的基因缺陷被认为具有多种有害影响,包括趋化性聚集减少、胞饮率降低和肌球蛋白- Arp2 /3-CP复合体组装效率低下。如果没有适当的CARMIL功能,细胞发育会因不恰当的肌动蛋白丝组装而受阻。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CARMIL 抗体 (E-10) 参考文献:

  1. 竹荪 CARMIL 蛋白通过其 SH3 结构域将盖帽蛋白和 Arp2/3 复合物与 I 型肌球蛋白连接起来。  |  Jung, G., et al. 2001. J Cell Biol. 153: 1479-97. PMID: 11425877
  2. CARMIL 是一种真正的盖层蛋白相互作用剂。  |  Remmert, K., et al. 2004. J Biol Chem. 279: 3068-77. PMID: 14594951
  3. 哺乳动物 CARMIL 通过盖层蛋白抑制肌动蛋白丝盖层。  |  Yang, C., et al. 2005. Dev Cell. 9: 209-21. PMID: 16054028
  4. 嗜中性粒细胞提取物中存在一种新型封顶蛋白抑制剂。  |  Huang, M., et al. 2005. Cell Motil Cytoskeleton. 62: 232-43. PMID: 16276529
  5. CARMIL 是一种强效的盖层蛋白拮抗剂:确定了一个保守的 CARMIL 结构域,该结构域可抑制盖层蛋白的活性并解除盖层肌动蛋白丝。  |  Uruno, T., et al. 2006. J Biol Chem. 281: 10635-50. PMID: 16434392
  6. 鉴定 CD2 相关蛋白中的新型抑制性肌动蛋白结合基团  |  Bruck, S., et al. 2006. J Biol Chem. 281: 19196-203. PMID: 16707503
  7. CKIP-1 在细胞形态中的作用取决于它与肌动蛋白的相互作用。  |  Canton, DA., et al. 2006. J Biol Chem. 281: 36347-59. PMID: 16987810
  8. 细胞外富含亮氨酸的重复超家族;进化关系和表达模式的比较调查和分析。  |  Dolan, J., et al. 2007. BMC Genomics. 8: 320. PMID: 17868438

订购信息

产品名称产品编号规格价格数量收藏夹

CARMIL 抗体 (E-10)

sc-365314
200 µg/ml
RMB2377.00

CARMIL (E-10): m-IgG Fc BP-HRP 套装

sc-527353
200 µg Ab; 10 µg BP
RMB2662.00

CARMIL (E-10): m-IgG1 BP-HRP 套装

sc-532726
200 µg Ab; 20 µg BP
RMB2662.00

CARMIL 抗体 (E-10) AC

sc-365314 AC
500 µg/ml, 25% agarose
RMB3129.00

CARMIL 抗体 (E-10) HRP

sc-365314 HRP
200 µg/ml
RMB2377.00

CARMIL 抗体 (E-10) FITC

sc-365314 FITC
200 µg/ml
RMB2482.00

CARMIL 抗体 (E-10) PE

sc-365314 PE
200 µg/ml
RMB2580.00

CARMIL 抗体 (E-10) Alexa Fluor® 488

sc-365314 AF488
200 µg/ml
RMB2685.00

CARMIL 抗体 (E-10) Alexa Fluor® 546

sc-365314 AF546
200 µg/ml
RMB2685.00

CARMIL 抗体 (E-10) Alexa Fluor® 594

sc-365314 AF594
200 µg/ml
RMB2685.00

CARMIL 抗体 (E-10) Alexa Fluor® 647

sc-365314 AF647
200 µg/ml
RMB2685.00

CARMIL 抗体 (E-10) Alexa Fluor® 680

sc-365314 AF680
200 µg/ml
RMB2685.00

CARMIL 抗体 (E-10) Alexa Fluor® 790

sc-365314 AF790
200 µg/ml
RMB2685.00

There are three CARMIL variants (1,2,3) and the molecular weights are reported to be 151k, 154k and 150kDa. What are the three bands on the western blot in the SCBT catalog that range from around 110kDa to 75kDa, based on your markers?

Asked by: Eastern Blot
Thank you for your question. The data on our website is of mouse transfected lysate, so it is not representing endogenous protein expression. According to Uniprot, there are possible expected isoforms for this protein at 152, 147, 55 and 20kDa. https://www.uniprot.org/uniprot/Q5VZK9
Answered by: Tech Service
Date published: 2020-01-28

Can CARMIL (E-10): sc-365314 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, CARMIL (E-10): sc-365314 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclearProduced beautiful immunoperoxidase nuclear envelope and nuclear staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Nice Western Blot data of CARMIL expression in nonNice Western Blot data of CARMIL expression in non-transfected and mouse CARMIL transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-06-20
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CARMIL 抗体 (E-10) is rated 5.0 out of 5 by 2.
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