Date published: 2025-9-8

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CAR 抗体 (E-1): sc-373791

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说明书
  • CAR 抗体 E-1 是小鼠单克隆 IgG2b κ,CAR抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫human物种的CAR的氨基酸1-300
  • CAR 抗体 (E-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CAR
  • 抗CAR抗体(E-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CAR (E-1): sc-373791免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是CAR Antibody (E-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CAR Antibody (E-1) 打包提供(请参阅下面的订购信息)。

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関連項目

CAR抗体(E-1)是一种IgG2bκ小鼠单克隆CAR抗体(也称为CXADR抗体、柯萨奇病毒腺病毒受体抗体、类似Ig的细胞粘附分子抗体、I型膜B组柯萨奇病毒和C亚组腺病毒受体抗体、CAR抗体、HCAR抗体或CAR4/6抗体),它通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CAR蛋白。CAR抗体(E-1)既可作为非偶联的抗CAR抗体形式,也可作为多种偶联形式的抗CAR抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。柯萨奇病毒和腺病毒受体(CAR)通过特异性蛋白相互作用结合各种腺病毒来介导病毒感染。病毒旋钮结构域与CAR的细胞外氨基末端结构域(称为D1)之间具有高亲和力。单独的D1结构域足以在转染细胞中结合旋钮。确定CAR和腺病毒之间的特异性相互作用对于进一步开发使用病毒载体的基因治疗是必不可少的。CAR在许多人类和小鼠细胞类型中表达。然而,低水平表达CAR的细胞不能被腺病毒感染。在某些细胞类型中避免这一问题的可能方法是补充CAR或修饰Ad旋钮以结合其他受体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CAR 抗体 (E-1) 参考文献:

  1. 腺病毒载体介导的基因转移和在人成纤维细胞中表达的 CAR 依赖性和 CAR 非依赖性途径。  |  Hidaka, C., et al. 1999. J Clin Invest. 103: 579-87. PMID: 10021467
  2. 在识别 CAR 的腺病毒科纤维蛋白上发现一个保守的受体结合位点。  |  Roelvink, PW., et al. 1999. Science. 286: 1568-71. PMID: 10567265
  3. 腺病毒与其人体细胞受体 CAR 结合机制的结构分析。  |  Bewley, MC., et al. 1999. Science. 286: 1579-83. PMID: 10567268
  4. 识别腺病毒 5 型纤维蛋白的接触残基并定义 CAR 结合位点。  |  Kirby, I., et al. 2000. J Virol. 74: 2804-13. PMID: 10684297
  5. 人类心力衰竭中柯萨奇病毒和腺病毒受体 mRNA 的表达。  |  Sasse, A., et al. 2003. J Gene Med. 5: 876-882. PMID: 14533196
  6. 泌尿生殖系统癌细胞中柯萨奇病毒和腺病毒受体(CAR)基因启动子的表观遗传调控。  |  Pong, RC., et al. 2003. Cancer Res. 63: 8680-6. PMID: 14695181
  7. 柯萨奇病毒和腺病毒受体结合消融可降低腺病毒的肝趋向性和毒性。  |  Yun, CO., et al. 2005. Hum Gene Ther. 16: 248-61. PMID: 15761264
  8. 柯萨奇病毒和腺病毒受体对结肠癌腺瘤-癌序列的影响。  |  Stecker, K., et al. 2011. Br J Cancer. 104: 1426-33. PMID: 21468049
  9. 人类柯萨奇病毒和腺病毒受体跨膜和细胞质结构域的天然变体对病毒内化没有影响。  |  Herrmann, L., et al. 2020. Biochem Biophys Res Commun. 527: 401-405. PMID: 32334832
  10. 细胞外囊泡-仿生囊泡改善了低柯萨奇病毒和腺病毒受体肿瘤中的溶瘤病毒-免疫疗法。  |  Zhang, Y., et al. 2020. Front Bioeng Biotechnol. 8: 574007. PMID: 33042975
  11. 一种具有转基因纤维的腺病毒载体通过利用柯萨奇病毒和腺病毒受体无关的细胞进入机制,显示出更强的滋养能力。  |  Dmitriev, I., et al. 1998. J Virol. 72: 9706-13. PMID: 9811704
  12. 柯萨奇病毒和腺病毒受体氨基末端免疫球蛋白 V 相关结构域可结合腺病毒 2 型和腺病毒 12 型的纤维结。  |  Freimuth, P., et al. 1999. J Virol. 73: 1392-8. PMID: 9882344

订购信息

产品名称产品编号规格价格数量收藏夹

CAR 抗体 (E-1)

sc-373791
200 µg/ml
$316.00

CAR (E-1): m-IgGκ BP-HRP 套装

sc-522501
200 µg Ab, 40 µg BP
$354.00

CAR (E-1): m-IgG2b BP-HRP 套装

sc-549039
200 µg Ab; 10 µg BP
$354.00

CAR 抗体 (E-1) AC

sc-373791 AC
500 µg/ml, 25% agarose
$416.00

CAR 抗体 (E-1) HRP

sc-373791 HRP
200 µg/ml
$316.00

CAR 抗体 (E-1) FITC

sc-373791 FITC
200 µg/ml
$330.00

CAR 抗体 (E-1) PE

sc-373791 PE
200 µg/ml
$343.00

CAR 抗体 (E-1) Alexa Fluor® 488

sc-373791 AF488
200 µg/ml
$357.00

CAR 抗体 (E-1) Alexa Fluor® 546

sc-373791 AF546
200 µg/ml
$357.00

CAR 抗体 (E-1) Alexa Fluor® 594

sc-373791 AF594
200 µg/ml
$357.00

CAR 抗体 (E-1) Alexa Fluor® 647

sc-373791 AF647
200 µg/ml
$357.00

CAR 抗体 (E-1) Alexa Fluor® 680

sc-373791 AF680
200 µg/ml
$357.00

CAR 抗体 (E-1) Alexa Fluor® 790

sc-373791 AF790
200 µg/ml
$357.00

Hi, Would it be possible to receive a trial vial of this antibody? Kind regards, Tessa

Asked by: TRKul
Thank you for your question. Please contact our technical service team to arrange for a sample vial of this antibody.
Answered by: Tech Support Europe
Date published: 2025-07-02

How can CAR (E-1): sc-373791 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Professor Griffin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-373791 HRP), phycoerythrin (sc-373791 PE), fluoroscein (sc-373791 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good anti-CAR antibodyWe used this antibody for western blot using mouse heart whole cell lysate, and for IF using frozen sectioned mouse heart. Both gave us publishable results.
Date published: 2020-06-27
Rated 5 out of 5 by from Good in IHCUsed in both human and mouse tissues, good staining.
Date published: 2017-10-19
Rated 5 out of 5 by from strong bandI obtained strong band use this antibody, the titer is very high , I tried 1:1000 ,clear band for us.Recommend use 4℃ overnight for blocking.
Date published: 2017-08-12
Rated 5 out of 5 by from Good Western blot data of CAR expression in nonGood Western blot data of CAR expression in non-transfected and human CAR transfected whole cell lysates. -SCBT QC
Date published: 2013-07-14
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CAR 抗体 (E-1) is rated 4.8 out of 5 by 4.
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