Date published: 2025-9-6
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CaMKKβ Antibody (C-11) - Western Blotting - Image 386141
CaMKKβ 抗体 (C-11) HRP: sc-271674 HRP. SK-N-SH (A), T98G (B), IMR-32 (C) 和 THP-1 (D) 全细胞裂解液中 CaMKKβ 表达的直接 western 印迹分析.
CaMKKβ 抗体 (C-11) HRP: sc-271674 HRP. SK-N-SH (A), T98G (B), IMR-32 (C) 和 THP-1 (D) 全细胞裂解液中 CaMKKβ 表达的直接 western 印迹分析.

CaMKKβ 抗体 (C-11): sc-271674

4.1(8)
寫評論提問

说明书
  • CaMKK beta 抗体 C-11 是小鼠单克隆 IgG1 κ，CaMKKβ抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-95位于human物种的CaMKKβ的N-terminus
  • CaMKK beta 抗体 (C-11) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 CaMKKβ
  • 抗CaMKK beta抗体（C-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CaMKKβ (C-11): sc-271674的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CaMKKβ Antibody (C-11) for WB applications. 的首选辅助检测试剂，这些试剂现与CaMKKβ Antibody (C-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外CaMK抗体，包括CaMKI, CaMKI δ, CaMKI γ, CaMKII, CaMKII α, CaMKII β, CaMKII δ, CaMKII γ, CaMKIV, CaMKK α, CaMKK β和CaMKV

CaMKKβ抗体（C-11）是一种IgG1 κ小鼠单克隆CaMKK beta抗体（也称为CaMKKbeta抗体、钙/钙调蛋白依赖性（CaM）激酶激酶beta抗体、钙/钙调蛋白依赖性蛋白激酶激酶2抗体、丝氨酸/苏氨酸特异性蛋白激酶家族成员CAMKK2抗体或Ca++/钙调蛋白依赖性蛋白激酶亚家族激酶2 beta抗体），可通过WB、IP、IF和ELISA检测人源的CaMKK beta蛋白质。CaMKKβ抗体（C-11）既可以是非偶联的抗CaMKK beta抗体形式，也可以是多种偶联形式的抗CaMKK beta抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ca2+/钙调蛋白依赖性蛋白激酶（CaM激酶）是结构上相关的丝氨酸/苏氨酸激酶亚家族，包括CaMKI、CaMKII和CaMKIV。CaMKI和CaMKIV受Ca2+和CaM刺激，但也需要通过CaMK进行磷酸化以实现完全激活。CaMKKα和CAMKKβ通过特异性磷酸化位于177位点的调节性苏氨酸残基来激活CaMKI。CAMKKβ还能够在200位点的苏氨酸残基上磷酸化CAMKIV。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CaMKKβ 抗体 (C-11) 参考文献:

  1. CaMKKβ-AMPK 信号复合体的特征。  |  Green, MF., et al. 2011. Cell Signal. 23: 2005-12. PMID: 21807092
  2. Circ_0000518 通过 FUS/CaMKKβ/AMPK 途径促进巨噬细胞/小胶质细胞 M1 极化，从而加重多发性硬化症。  |  Jiang, F., et al. 2022. Neuroscience. 490: 131-143. PMID: 34920022
  3. 槲皮素通过CaMKKβ/AMPK/mTOR信号通路诱导HL-60细胞自噬相关死亡。  |  Xiao, J., et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54: 1244-1256. PMID: 36148953
  4. Bufalin通过Ca2+/CaMKKβ/AMPK/Beclin1信号通路诱导骨肉瘤细胞凋亡和自噬。  |  Zheng, YD., et al. 2023. Cell Biol Int. 47: 1344-1353. PMID: 36987947
  5. 对精子中与 CaMKIV 共定位的 CaMKKβ/2 剪接变体进行转录物、生物化学和免疫组织化学分析。  |  Ohtsuka, S., et al. 2024. Cell Calcium. 117: 102820. PMID: 37979343
  6. 人类钙钙调蛋白依赖性蛋白激酶 I：cDNA 克隆, 结构域结构和钙钙调蛋白依赖性蛋白激酶 I 激酶在苏氨酸-177 处磷酸化的活化。  |  Haribabu, B., et al. 1995. EMBO J. 14: 3679-86. PMID: 7641687
  7. 钙离子/钙调蛋白依赖性蛋白激酶级联的特征。钙/钙调蛋白依赖性蛋白激酶激酶的分子克隆和表达。  |  Tokumitsu, H., et al. 1995. J Biol Chem. 270: 19320-4. PMID: 7642608
  8. 钙/钙调蛋白依赖性蛋白激酶II能下调人类T细胞中钙调素和蛋白激酶C介导的细胞因子基因转录途径。  |  Hama, N., et al. 1995. J Exp Med. 181: 1217-22. PMID: 7869038
  9. 人钙调素依赖性蛋白激酶 IV 的 cDNA 克隆和表达。  |  Kitani, T., et al. 1994. J Biochem. 115: 637-40. PMID: 8089075
  10. KN-93 是 CaMK-II（多功能 Ca2+/CaM 激酶）的一种阻抑剂，可诱导 NIH 3T3 细胞的 G1 细胞周期停滞和细胞凋亡。  |  Tombes, RM., et al. 1995. Cell Growth Differ. 6: 1063-70. PMID: 8519682
  11. Jurkat T 淋巴细胞中 CaM 激酶对 Ca2+/calmodulin 依赖性蛋白激酶（CaM-激酶）IV 的激活。  |  Park, IK. and Soderling, TR. 1995. J Biol Chem. 270: 30464-9. PMID: 8530476
  12. 钙调蛋白依赖性蛋白激酶级联的组成部分。钙调素依赖性蛋白激酶激酶 beta 的分子克隆, 功能表征和细胞定位。  |  Anderson, KA., et al. 1998. J Biol Chem. 273: 31880-9. PMID: 9822657

订购信息

产品名称产品编号规格价格数量收藏夹

CaMKKβ 抗体 (C-11)

sc-271674
200 µg/ml
RMB2377.00

CaMKKβ (C-11): m-IgG Fc BP-HRP 套装

sc-527275
200 µg Ab; 10 µg BP
RMB2662.00

CaMKKβ (C-11): m-IgG1 BP-HRP 套装

sc-532648
200 µg Ab; 20 µg BP
RMB2662.00

CaMKKβ 抗体 (C-11) AC

sc-271674 AC
500 µg/ml, 25% agarose
RMB3129.00

CaMKKβ 抗体 (C-11) HRP

sc-271674 HRP
200 µg/ml
RMB2377.00

CaMKKβ 抗体 (C-11) FITC

sc-271674 FITC
200 µg/ml
RMB2482.00

CaMKKβ 抗体 (C-11) PE

sc-271674 PE
200 µg/ml
RMB2580.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 488

sc-271674 AF488
200 µg/ml
RMB2685.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 546

sc-271674 AF546
200 µg/ml
RMB2685.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 594

sc-271674 AF594
200 µg/ml
RMB2685.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 647

sc-271674 AF647
200 µg/ml
RMB2685.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 680

sc-271674 AF680
200 µg/ml
RMB2685.00

CaMKKβ 抗体 (C-11) Alexa Fluor® 790

sc-271674 AF790
200 µg/ml
RMB2685.00

What blocking solution should I use with CaMKKβ (C-11): sc-271674 monoclonal antibody for Western blot?

Asked by: jenniferc
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 1 out of 5 by from This is not working in H9c2, rat-cardiomyoblast.I used it for WB, 1:500, in H9c2 cell, rat-cardiomyoblast several times; however, I got only very very tiny bands. I will get another CAMKK2 antibody.
Date published: 2022-06-15
Rated 3 out of 5 by from Works in WB. Human MSCsWeak band can be detected by Western blot in human MSCs.
Date published: 2017-09-18
Rated 5 out of 5 by from good signal resultThe antibody been tested in Human breast cell lines, there was specific signal at 66kda,after,incubate in RT for 1 hour, the band is very strong even diluted 1:1000,after long time exposure 1hour,there was a little background,so I recommend exposed short time.
Date published: 2017-07-23
Rated 5 out of 5 by from strong signal for WBgood quality antibody and clear signal, no background, the band is very clean for WB.
Date published: 2017-06-03
Rated 5 out of 5 by from Good Western Blot ResultsWE used this antibody in a western blot for human samples and got a clear distinct band
Date published: 2016-12-22
Rated 5 out of 5 by from I got resultsI got results, it worked well, no issues, 5 stars
Date published: 2015-04-07
Rated 5 out of 5 by from Worked well for WB in H4Worked well for WB in H4, SK-N-SH, T98G, and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-02-18
Rated 4 out of 5 by from Robust band in WB in IMRRobust band in WB in IMR-32 whole cell lysate. -SCBT QC
Date published: 2014-01-04
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CaMKKβ 抗体 (C-11) is rated 4.1 out of 5 by 8.
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